Qishen Yiqi dripping pills for chronic ischaemic heart failure:results of the CACT-IHF randomized clinical trial

10.1002/ehf2.12980ESC Heart Failure763881-389

Epigenetic Modification of TLRs in Leukocytes Is Associated with Increased Susceptibility to Salmonella enteritidis in Chickens

Toll-like receptors (TLRs) signaling pathways are the first lines in defense against Salmonella enteritidis (S. enteritidis) infection but the molecular mechanism underlying susceptibility to S. enteritidis infection in chicken remains unclear. SPF chickens injected with S. enteritidis were partitioned into two groups, one consisted of those from Salmonella-susceptible chickens (died within 5 d after injection, n = 6), the other consisted of six Salmonella-resistant chickens that survived for 15 d after injection. The present study shows that the bacterial load in susceptible chickens was significantly higher than that in resistant chickens and TLR4, TLR2-1 and TLR21 expression was strongly diminished in the leukocytes of susceptible chickens compared with those of resistant chickens. The induction of expression of pro-inflammatory cytokine genes, IL-6 and IFN-β, was greatly enhanced in the resistant but not in susceptible chickens. Contrasting with the reduced expression of TLR genes, those of the zinc finger protein 493 (ZNF493) gene and Toll-interacting protein (TOLLIP) gene were enhanced in the susceptible chickens. Finally, the expression of TLR4 in peripheral blood mononuclear cells (PBMCs) infected in vitro with S. enteritidis increased significantly as a result of treatment with 5-Aza-2-deoxycytidine (5-Aza-dc) while either 5-Aza-dc or trichostatin A was effective in up-regulating the expression of TLR21 and TLR2-1. DNA methylation, in the predicted promoter region of TLR4 and TLR21 genes, and an exonic CpG island of the TLR2-1 gene was significantly higher in the susceptible chickens than in resistant chickens. Taken together, the results demonstrate that ZNF493-related epigenetic modification in leukocytes probably accounts for increased susceptibility to S. enteritidis in chickens by diminishing the expression and response of TLR4, TLR21 and TLR2-1

Influence of the X-point location on edge plasma transport in the J-TEXT tokamak with a high-field-side single-null divertor

High-density experiments in the high-field-side mid-plane single-null divertor configuration have been performed for the first time on J-TEXT. The experiments show an increase in the highest central channel line-averaged density from $2.73\,\times \,{10}^{19}\,{{\rm{m}}}^{-3}$ to $6.49\,\times \,{10}^{19}\,{{\rm{m}}}^{-3}$, while the X-point moves away from the target by increasing the divertor coil current. The corresponding Greenwald fraction rises from 0.50 to 0.79. For the impurity transport, the density normalized radiation intensity (absolute extreme ultraviolet and soft x-ray) of the central channel density decreased significantly (>50%) with an increase in the plasma density. To better understand the underlying physics mechanisms, the 3D edge Monte Carlo code coupled with EIRENE (EMC3- EIRENE) has been implemented for the first time on J-TEXT. The simulation results show good agreement with the experimental findings. As the X-point moves away from the target, the divertor power decay length drops and the scrape-off layer impurity screening effect is enhanced