Biochemical Evaluation of Withania Somnifera Root Powder on Adjuvant-Induced Arthritis in Rats

The present investigation was carried out to evaluate the biochemical effect of Withania somnifera Linn. Solanaceae, commonly known as ashwagandha on adjuvant induced arthritic rats. Results were compared to Indomethacin, a non steroidal anti-inflammatory drug. Arthritis was induced by an intra dermal injection of Complete Freund’s Adjuvant (0.1 ml) into the right hind paw of Wistar albino rats. Withania somnifera root powder (1000 mg/kg/day) and Indomethacin (3 mg/kg/day) were orally administered for 8 days (from 11th to 18th day) after adjuvant injection. After the experimental period, all the animals were sacrificed and serum, liver and spleen samples were collected for further biochemical analysis. A significant increase in the activities of gluconeogenic enzymes, tissue marker enzymes, blood glucose level, WBC, platelet count, erythrocyte sedimentation rate, and acute phase proteins (hyaluronic acid, fibrinogen and ceruloplasmin) was observed in adjuvant-induced arthritic rats, whereas the activities of glycolytic enzymes, body weight, levels of hemoglobin, RBC count, and packed cell volume were found to be decreased. These biochemical alterations observed in arthritic animals were ameliorated significantly after the administration of Withania somnifera root powder (1000 mg/kg/b.wt) and Indomethacin (3 mg/kg/b.wt). Our results suggest that Withania somnifera root powder is capable of rectifying the above biochemical changes in adjuvant arthritis and it may prove to be useful in treating rheumatoid arthritis

A Multilayered Clustering Framework to build a Service Portfolio using Swarm-based algorithms

In this paper, a multilayered clustering framework is proposed to build a service portfolio to select web services of choice. It is important for every service provider to create a service portfolio in order to facilitate the service selection process for someone to obtain the desired service in the absence of public UDDI registries. To address this problem, a multilayered clustering approach applied on a variety of data pertaining to web services in order to filter and group the services of a similar kind which in turn will improve the leniency in the process of service selection is used. The advantages of the layer approach are reduced search space, combination of incremental learning and competitive learning strategies, reduced computational labour, scalability, robustness and fault tolerance. The results are subjected to cluster analysis to verify their degree of compactness and isolation and appropriate evaluation indices are used. The results were found passable with an improved degree of similarity

Assessment of soil and water quality status of rose growing areas of Rajasthan and Uttar Pradesh, India

Rose is a commercial flower crop widely grown across India. It is highly sensitive to salinity and alkalinity. In the process of identification of salt and alkalinity resistant rootstocks of rose cultivars, a survey was conducted in the rose growing areas of Uttar Pradesh (UP) and Rajasthan. Total of 28 representative surface soil samples were collected from rose fields of these regions, processed and analyzed for the soil quality parameters. Similarly water samples (20 samples) from the bore wells of these fields were collected and analyzed. The results revealed that most of the soils of rose growing fields in UP were alkaline (pH >8.0) with normal salt content (electrical conductivity, EC < 0.5 dS m-1). Many of these soils also had higher bicarbonates (> 3 meq 100 g-1). In case of Rajasthan, few samples had higher pH, EC, chloride (>2 meq 100 g-1) and bicarbonate contents. Exchangeable sodium percentage (ESP) of UP and Rajasthan samples ranged from 5.21-20.7% and 2.94-24.9%, respectively. In case of water parameters in these areas, pH was slightly in alkaline range, EC of some of the samples were high (>1 dSm-1). Sodium content was slightly higher than other cations. Soluble sodium percentage (SSP) of water samples was also slightly higher than normal range (0-50%). Few samples had slightly higher chloride above the threshold limit. From the results, it is concluded that soil and water quality of the rose growing areas of UP and Rajasthan is marginal and proper management/reclamation measures need to be carried out for sustaining the production system

Environmental Risks Associated with Heavy Metal Contamination in Soil, Water and Plants in Urban and Periurban Agriculture

The India's population living in cities and urban areas has doubled to 27.8% since Independence. Our cities face enormous challenges of environmental pollution and health related problems. City authorities have often been reluctant to accept urban and periurban agriculture because of perceived health risks. Nevertheless, in most cities the world over, periurban agriculture is practiced on a substantial scale, despite prohibitive laws and regulations. Non-degradable pollutants added to the system through anthropogenic activities like heavy metals in air, soil, water and crops bother us more than others as these tend to bio-accumulate. Throughout history, heavy metal contamination has long plagued mankind - undermining intelligence and causing debasing behaviour. Toxicity of some of the heavy metals even leads to deficiency of essential metals like Zn, Cu, etc. in both human and animals. Climate, nutritional status, genetic predisposition, type of work and exposure level influence the intensity of impact on health. Permissible levels prescribed by different organizations differ because of differences in tolerance levels of people of different origins and differences in threat perception of the people. With our current level of knowledge a permanent and foolproof method to stop entry of heavy metals into the food chain is impossible. However, methods are available to reduce intensity of the effects. Alternative land use with crops not directly consumed by human beings and animals offers a better remedy to contain heavy metal entry into food chain. India has a wide ranging set of environmental laws that lay down norms for air, water, soil, wastes, etc. Legislative frame work has been developed in the belief that a policing model is sufficient. It does not go beyond that. Regulatory mechanisms may not be effective in isolated cases but are essential drivers to augment other approaches, by putting a "cap" on the level of degradation that is socially acceptable, as well as creating space for other, cleaner and more acceptable alternatives to be "viable"

XDLX: A Memory-Efficient Solution for Backtracking Applications in Big Data Environment using XOR-based Dancing Links

Interactive and Backtracking applications often require undoing certain recent operations and updates made to the underlying data structures. The concept of dancing links has made such reverting operations easier and efficient by repeatedly performing unlinking and re-linking of pointers in complex data structures involving circular multiply linked lists. This paper extends the idea of dancing links to XOR linked lists, the memory efficient counterpart of doubly linked lists to develop XDLX, a more space efficient algorithm than DLX to solve exact cover problems without compromising the timing efficiency. Owing to the NP-Complete nature of the exact cover problem, any NP-complete problem can be reduced to it and solved using the proposed memory-efficient dancing links based algorithm, XDLX. The algorithm can be effectively used to solve any backtracking application and will prove to be a significant contribution towards the programming of memory-constrained environments such as embedded systems

Adaptive regulation of riboflavin transport in heart: effect of dietary riboflavin deficiency in cardiovascular pathogenesis

Deficiency or defective transport of riboflavin (RF) is known to cause neurological disorders, cataract, cardiovascular anomalies, and various cancers by altering the biochemical pathways. Mechanisms and regulation of RF uptake process is well characterized in the cells of intestine, liver, kidney, and brain origin, while very little is known in the heart. Hence, we aimed to understand the expression and regulation of RF transporters (rRFVT-1 and rRFVT-2) in cardiomyocytes during RF deficiency and also investigated the role of RF in ischemic cardiomyopathy and mitochondrial dysfunction in vivo. Riboflavin uptake assay revealed that RF transport in H9C2 is (1) significantly higher at pH 7.5, (2) independent of Na+ and (3) saturable with a Km of 3.746 µM. For in vivo studies, male Wistar rats (110–130 g) were provided riboflavin deficient food containing 0.3 ± 0.05 mg/kg riboflavin for 7 weeks, which resulted in over expression of both RFVTs in mRNA and protein level. RF deprivation resulted in the accumulation of cardiac biomarkers, histopathological abnormalities, and reduced mitochondrial membrane potential which evidenced the key role of RF in the development of cardiovascular pathogenesis. Besides, adaptive regulation of RF transporters upon RF deficiency signifies that RFVTs can be considered as an effective delivery system for drugs against cardiac diseases

3′-UTR SNP rs2229611 in G6PC1 affects mRNA stability, expression and Glycogen Storage Disease type-Ia risk

The frequency of rs2229611, previously reported in Chinese, Caucasians, Japanese and Hispanics, was investigated for the first time in Indian ethnicity. We analyzed its role in the progression of Glycogen Storage Disease type-Ia (GSD-Ia) and breast cancer. Genotype data on rs2229611 revealed that the risk of GSD-Ia was higher (P = 0.0195) with CC compared to TT/TC genotypes, whereas no such correlation was observed with breast cancer cases. We observed a strong linkage disequilibrium (LD) among rs2229611 and other disease causing G6PC1 variants (| D′| = 1, r2 = 1). Functional validation performed in HepG2 cells using luciferase constructs showed significant (P < 0.05) decrease in expression than wild-type 3′-UTR due to curtailed mRNA stability. Furthermore, AU-rich elements (AREs) mediated regulation of G6PC1 expression characterized using 3′-UTR deletion constructs showed a prominent decrease in mRNA stability. We then examined whether miRNAs are involved in controlling G6PC1 expression using pmirGLO-UTR constructs, with evidence of more distinct inhibition in the reporter function with rs2229611. These data suggests that rs2229611 is a crucial regulatory SNP which in homozygous state leads to a more aggressive disease phenotype in GSD-Ia patients. The implication of this result is significant in predicting disease onset, progression and response to disease modifying treatments in patients with GSD-Ia