13 research outputs found
<span style="font-size:12.0pt;font-family:"Times New Roman";mso-bidi-font-weight: bold" lang="EN-US">Diversity and antimicrobial activity of endophytic fungi isolated from <span style="font-size:12.0pt;font-family:"Times New Roman";mso-bidi-font-weight: bold" lang="EN-US">the seagrass <i>Enhalus acoroides</i> </span></span>
785-797Endophytic fungi were
isolated from the seagrass, Enhalus acoroides, collected from Trang
province, Thailand.
Forty-seven endophytic isolates cultured were classified into 17
phylogenetically diverse genera based on their morphology and molecular
analysis of the ITS regions of the rDNA. Most common species were Penicillium
(6 isolates), Nigrospora
(5), and Fusarium (4)
and 2 with unknown taxonomic affinity. Crude extracts including culture media
and cells of all isolates were tested for their antimicrobial activities using
a colorimetric broth microdilution method against ten potential human
pathogens. Extracts from 38 isolates (80.85%) showed antimicrobial activity
with minimum inhibitory concentration (MIC) values ranging from 4 to 200 ÎĽg mL-1.
Nigrospora sp. PSU-ES5 produced the most active extracts against Microsporum
gypseum (MIC 4 to 8 ÎĽg mL-1). Endophytic fungi from seagrasses
such as E. acoroides could therefore be a good source for obtaining
antimicrobial natural products