Flow cytometric detection of gamma interferon can effectively discriminate Mycobacterium bovis BCG-vaccinated cattle from M. bovis-infected cattle

Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that is increasing in incidence in United Kingdom cattle herds. In addition to increasing economic losses, the rise in bovine tuberculosis poses a human health risk. There is an urgent requirement for effective strategies for disease eradication; this will likely involve vaccination in conjunction with current test and slaughter policies. A policy involving vaccination would require an accurate diagnosis of M. bovis-infected animals and the potential to distinguish these animals from vaccinates. Currently used diagnostic tests, the skin test and gamma interferon (IFN-γ) blood test, have a sensitivity of up to 95%. A further complication is that M. bovis BCG-vaccinated animals are also scored positive by these tests. We tested the hypothesis that the quantification of IFN-γ-producing lymphocytes by flow cytometric analysis of intracellular IFN-γ expression would provide a more accurate discrimination of M. bovis-infected animals from BCG vaccinates. Significant numbers of IFN-γ-expressing CD4(+) T cells were detected following culture of heparinized blood from M. bovis-infected animals, but not from BCG vaccinates, with purified protein derived from M. bovis (PPD-B) or live mycobacteria. Only 1 of 17 BCG-vaccinated animals had a significant number of CD4(+) T lymphocytes expressing IFN-γ, compared with 21/22 M. bovis-infected animals. This assay could allow an accurate diagnosis of M. bovis and allow the discrimination of BCG-vaccinated cattle from infected cattle

Probing Cool and Warm Infrared Galaxies using Photometric and Structural Measures

We have analyzed a sample of nearby cool and warm infrared (IR) galaxies using photometric and structural parameters. The set of measures include far-infrared color ($C = \log_{10}[S_{60\mu m}/S_{100\mu m}]$), total IR luminosity ($L_{TIR}$), radio surface brightness as well as radio, near-infrared, and optical sizes. In a given luminosity range cool and warm galaxies are considered as those sources that are found approximately $1 \sigma$ below and above the mean color in the far-infrared $C - L_{TIR}$ diagram. We find that galaxy radio surface brightness is well correlated with color whereas size is less well correlated with color. Our analysis indicates that IR galaxies that are dominated by cool dust are large, massive spirals that are not strongly interacting or merging and presumably the ones with the least active star formation. Dust in these cool objects is less centrally concentrated than in the more typical luminous and ultra-luminous IR galaxies that are dominated by warm dust. Our study also shows that low luminosity early type unbarred and transitional spirals are responsible for the large scatter in the $C - L_{TIR}$ diagram. Among highly luminous galaxies, late type unbarred spirals are predominately warm, and early type unbarred and barred are systematically cooler. We highlight the significance of $C - L_{TIR}$ diagram in terms of local and high redshifts sub-millimeter galaxies.Comment: Accepted for publication in ApJ, 2006, 23 pages, 3 postscript figures, 1 table. The table can be obtained on request from the author

The optically-powerful quasar E1821+643 is associated with a 300-kpc scale FRI radio structure

We present a deep image of the optically-powerful quasar E1821+643 at 18cm made with the Very Large Array (VLA). This image reveals radio emission, over 280 kpc in extent, elongated way beyond the quasar's host galaxy. Its radio structure has decreasing surface brightness with increasing distance from the bright core, characteristic of FRI sources (Fanaroff & Riley 1974). Its radio luminosity at 5GHz falls in the classification for `radio-quiet' quasars (it is only 10^23.9 W/Hz/sr; see e.g. Kellermann et al 1994). Its radio luminosity at 151MHz (which is 10^25.3 W/Hz/sr) is at the transition luminosity observed to separate FRIs and FRIIs. Hitherto, no optically-powerful quasar had been found to have a conventional FRI radio structure. For searches at low-frequency this is unsurprising given current sensitivity and plausible radio spectral indices for radio-quiet quasars. We demonstrate the inevitability of the extent of any FRqI radio structures being seriously under-estimated by existing targetted follow-up observations of other optically-selected quasars, which are typically short exposures of z > 0.3 objects, and discuss the implications for the purported radio bimodality in quasars. The nature of the inner arcsec-scale jet in E1821+643, together with its large-scale radio structure, suggest that the jet-axis in this quasar is precessing (cf. Galactic jet sources such as SS433). A possible explanation for this is that its central engine is a binary whose black holes have yet to coalesce. The ubiquity of precession in `radio-quiet' quasars, perhaps as a means of reducing the observable radio luminosity expected in highly-accreting systems, remains to be established.Comment: Accepted by ApJ Letters; higher quality versions of figures available at http://www-astro.physics.ox.ac.uk/~km

Radio Variability of Radio Quiet and Radio Loud Quasars

The majority of quasars are weak in their radio emission, with flux densities comparable to those in the optical, and energies far lower. A small fraction, about 10%, are hundreds to thousands of times stronger in the radio. Conventional wisdom holds that there are two classes of quasars, the radio quiets and radio louds, with a deficit of sources having intermediate power. Are there really two separate populations, and if so, is the physics of the radio emission fundamentally different between them? This paper addresses the second question, through a study of radio variability across the full range of radio power, from quiet to loud. The basic findings are that the root mean square amplitude of variability is independent of radio luminosity or radio-to-optical flux density ratio, and that fractionally large variations can occur on timescales of months or less in both radio quiet and radio loud quasars. Combining this with similarities in other indicators, such as radio spectral index and the presence of VLBI-scale components, leads to the suggestion that the physics of radio emission in the inner regions of all quasars is essentially the same, involving a compact, partially opaque core together with a beamed jet.Comment: 32 pages, 9 figures. Astrophysical Journal, in pres

The Sunyaev-Zel'dovich Effect by Cocoons of Radio Galaxies

We estimate the deformation of the cosmic microwave background radiation by the hot region (``cocoon'') around a radio galaxy. A simple model is adopted for cocoon evolution while the jet is on, and a model of evolution is constructed after the jet is off. It is found that at low redshift the phase after the jet is off is longer than the lifetime of the jets. The Compton y-parameter generated by cocoons is calculated with a Press-Schechter number density evolution. The resultant value of y is of the same order as the COBE constraint. The Sunyaev-Zeldovich effect due to cocoons could therefore be a significant foreground source of small angular scale anisotropies in the cosmic microwave background radiation.Comment: Published version, 23 pages with 5 figure

Interferometric Observations of the Nuclear Region of Arp220 at Submillimeter Wavelengths

We report the first submillimeter interferometric observations of an ultraluminous infrared galaxy. We observed Arp220 in the CO J=3-2 line and 342GHz continuum with the single baseline CSO-JCMT interferometer consisting of the Caltech Submillimeter Observatory (CSO) and the James Clerk Maxwell Telescope (JCMT). Models were fit to the measured visibilities to constrain the structure of the source. The morphologies of the CO J=3-2 line and 342GHz continuum emission are similar to those seen in published maps at 230 and 110GHz. We clearly detect a binary source separated by about 1 arcsec in the east-west direction in the 342GHz continuum. The CO J=3-2 visibility amplitudes, however, indicate a more complicated structure, with evidence for a compact binary at some velocities and rather more extended structure at others. Less than 30% of the total CO J=3-2 emission is detected by the interferometer, which implies the presence of significant quantities of extended gas. We also obtained single-dish CO J=2-1, CO J=3-2 and HCN J=4-3 spectra. The HCN J=4-3 spectrum, unlike the CO spectra, is dominated by a single redshifted peak. The HCN J=4-3/CO J=3-2, HCN J=4-3/HCN J=1-0 and CO J=3-2/2-1 line ratios are larger in the redshifted (eastern) source, which suggests that the two sources may have different physical conditions. This result might be explained by the presence of an intense starburst that has begun to deplete or disperse the densest gas in the western source, while the eastern source harbors undispersed high density gas.Comment: 17 pages, 9 figures, 4 Tables. accepted by Ap

A relativistic jet in the radio-quiet quasar PG1407+263

We present the results of a multi-epoch radio monitoring campaign measuring the milliarcsecond structure of the jet in the radio-quiet quasar PG1407+263. This is the highest-sensitivity, highest-resolution multi-year study of a distant active galaxy. The observations are naturally explained in terms of a beamed relativistic jet, some of whose fluctuations in flux density can be ascribed to interaction with the narrow-line region of the quasar. The optical properties of PG1407+263, in particular the low equivalent widths of the emission lines, may be related to the fact that we are viewing this quasar almost pole-on, giving us a direct view into its broad-line region.Comment: 4 pages, uses emulateapj5.sty; accepted by ApJ Letter

Sensitive observations at 1.4 and 250 GHz of z > 5 QSOs

We present 1.4 and 5 GHz observations taken with the Very Large Array (VLA), and observations at 250 GHz obtained with the Max-Planck millimeter bolometer (MAMBO) at the IRAM 30~m telescope, of ten optically selected Quasi-stellar Objects (QSOs) at 5.0 < z < 6.28. Four sources are detected at 1.4 GHz two of which are radio loud and are also detected at 5 GHz. These results are roughly consistent with there being no evolution of the radio-loud QSO fraction out to z~6. Three sources have been detected at 250 GHz or 350 GHz at much higher levels than their 1.4 GHz flux densities suggesting that the observed mm emission is likely thermal emission from warm dust, although more exotic possibilities cannot be precluded. The highest redshift source in our sample (J1030+0524 at z=6.28) is not detected at 1.4 or 250 GHz, but four fairly bright radio sources (flux density at 1.4GHz > 0.2 mJy) are detected in a 2' field centered on the QSO, including an edge-brightened ('FRII') double radio source with an extent of about 1'. A similar over-density of radio sources is seen in the field of the highest redshift QSO J1148+5251. We speculate that these over-densities of radio sources may indicate clusters along the lines-of-sight, in which case gravitational lensing by the cluster could magnify the QSO emission by a factor 2 or so without giving rise to arcsecond-scale distortions in the optical images of the QSOs.Comment: 25 pages, 12 figures. accepted by A

Rotating Nuclear Rings and Extreme Starbursts in Ultraluminous Galaxies

New high resolution interferometer data of 10 IR ultraluminous galaxies shows the molecular gas is in rotating nuclear rings or disks with radii 300 to 800 pc. Most of the CO flux comes from a moderate-density, warm, intercloud medium rather than self-gravitating clouds. Gas masses of ~ 5 x 10^9 Msun, 5 times lower than the standard method are derived from a model of the molecular disks. The ratio of molecular gas to dynamical mass, is M_gas/M_dyn ~ 1/6 with a maximum ratio of gas to total mass surface density of 1/3. For the galaxies VIIZw31, Arp193, and IRAS 10565+24, there is good evidence for rotating molecular rings with a central gap. In addition to the rotating rings a new class of star formation region is identified which we call an Extreme Starburst. They have a characteristic size of only 100 pc., about 10^9 Msun of gas and an IR luminosity of ~3 x 10^11 Lsun. Four extreme starbursts are identified in the 3 closest galaxies in the sample Arp220, Arp193 and Mrk273. They are the most prodigious star formation events in the local universe, each representing about 1000 times as many OB stars as 30 Doradus. In Arp220, the CO and 1.3 mm continuum maps show the two ``nuclei'' embedded in a central ring or disk and a fainter structure extending 3 kpc to the east, normal to the nuclear disk. There is no evidence that these sources really are the pre-merger nuclei. They are compact, extreme starburst regions containing 10^9 Msun of dense molecular gas and new stars, but no old stars. Most of the dust emission and HCN emission arises in the two extreme starbursts. The entire bolometric luminosity of Arp~220 comes from starbursts, not an AGN. In Mrk231, the disk geometry shows that the molecular disk cannot be heated by the AGN; the far IR luminosity of Mrk~231 is powered by a starburst, not the AGN. (Abridged)Comment: 97 pages Latex with aasms.sty, including 29 encapsulated Postscript figures. Figs 18 and 23 are GIFs. 31 figures total. Text and higher quality versions of figures available at http://sbastk.ess.sunysb.edu/www/RINGS_ESB_PREPRINT.html To be published in Ap. J., 10 Nov. 199