94 research outputs found
22: Ex-vivo expansion (EvE) of previously cryopreserved cord blood (CB) into natural killer (NK) cells with enhanced AML and neuroblastoma cytotoxicity Potential role of CB NK cells in adoptive cellular immunotherapy (ACI)
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Prevention of invasive Mold infection Post Allogeneic Stem Cell Transplantation (AlloSCT) in Pediatric Recipients Using the Sequential Combination of Liposomal Amphotericin B (Ambisome®) Followed by Micafungin (Mycamine®)
Romedepsin (RM), A Hdaci, Significantly Increases The Expression Of NKG2D Ligands, MIC A/B, In Leukemia/Lymphoma Cells (LL), In Part Through The Glycogen Synthase Kinase-3 (GSK-3) Pathway, Resulting In Enhanced NK Cytotoxicity: Translational Approach For Adoptive NK Cell Immunotherapy
Differential Genomic Expression Of Nk Receptor (NKR) In Cord Blood (CB) CD56dim Versus Peripheral Blood (PB) CD56dim Nk Cells: Implication for Immaturity In Cord Blood Nk CD56dim Innate Immunity
Low Day 100 Transplant-Related Mortality (TRM) Following Clofarabine (CLO) in Combination with Cytarabine and Total Body Irradiation (TBI), Myeloablative Conditioning (MAC) and Allogeneic Stem Cell Transplantation (AlloSCT) in Children, Adolescents and Young Adults (CAYA) with Poor-Risk Acute Leukemia
341: Reduced-intensity allogeneic umbilical cord blood transplantation (RI-UCBT) in pediatric recipients with malignant and non-malignant diseases
164: Risk adapted allogeneic stem cell transplantation (AlloSCT) for acquired severe aplastic anemia (SAA) in pediatric recipients
229: Twice-Daily Intravenous (IV) Busulfan (Bu) x 4 Days in Children Undergoing a Reduced-Intensity Conditioning (RIC) Regimen with Allogeneic Hematopoietic Stem Cell Transplantation (AlloHSCT) is Safe and Well-Tolerated but Results in Significantly Increased Bu Clearance (CL) and Decreased Area Under the Curve (AUC) and Half-Life (t1/2) When Compared to IV Bu Twice-Daily Dosing Pharmacokinetics (PK) in Adults
Disease burden and conditioning regimens in ASCT1221, a randomized phase II trial in children with juvenile myelomonocytic leukemia: A Children's Oncology Group study
Background: Most patients with juvenile myelomonocytic leukemia (JMML) are curable only with allogeneic hematopoietic cell transplantation (HCT). However, the current standard conditioning regimen, busulfan-cyclophosphamide-melphalan (Bu-Cy-Mel), may be associated with higher risks of morbidity and mortality. ASCT1221 was designed to test whether the potentially less-toxic myeloablative conditioning regimen containing busulfan-fludarabine (Bu-Flu) would be associated with equivalent outcomes. Procedure: Twenty-seven patients were enrolled on ASCT1221 from 2013 to 2015. Pre- and post-HCT (starting Day +30) mutant allele burden was measured in all and pre-HCT therapy was administered according to physician discretion. Results: Fifteen patients were randomized (six to Bu-Cy-Mel and nine to Bu-Flu) after meeting diagnostic criteria for JMML. Pre-HCT low-dose chemotherapy did not appear to reduce pre-HCT disease burden. Two patients, however, received aggressive chemotherapy pre-HCT and achieved low disease-burden state; both are long-term survivors. All four patients with detectable mutant allele burden at Day +30 post-HCT eventually progressed compared to two of nine patients with unmeasurable allele burden (P = 0.04). The 18-month event-free survival of the entire cohort was 47% (95% CI, 21–69%), and was 83% (95% CI, 27–97%) and 22% (95% CI, 03–51%) for Bu-Cy-Mel and Bu-Flu, respectively (P = 0.04). ASCT1221 was terminated early due to concerns that the Bu-Flu arm had inferior outcomes. Conclusions: The regimen of Bu-Flu is inadequate to provide disease control in patients with JMML who present to HCT with large burdens of disease. Advances in molecular testing may allow better characterization of biologic risk, pre-HCT responses to chemotherapy, and post-HCT management
Phagocytic ability of neutrophils and monocytes in neonates
<p>Abstract</p> <p>Background</p> <p>Infections by a variety of pathogens are a significant cause of morbidity and mortality during perinatal period. The susceptibility of neonates to bacterial infections has been attributed to immaturity of innate immunity. It is considered that one of the impaired mechanisms is the phagocytic function of neutrophils and monocytes. The purpose of the present study was to investigate the phagocytic ability of neonates at birth.</p> <p>Methods</p> <p>The phagocytic ability of neutrophils and monocytes of 42 neonates was determined using the Phagotest flow cytometry method, that assesses the intake of <it>E. Coli </it>by phagocytes, in cord blood and in peripheral blood 3 days after birth. Fifteen healthy adults were included in the study as controls.</p> <p>Results</p> <p>The phagocytic ability of neutrophils in the cord blood of neonates was significantly reduced compared to adults. The 3<sup>rd </sup>postnatal day the reduction of phagocytic ability of neutrophils was no longer significant compared to adults. The phagocytic ability of monocytes did not show any difference from that of adults either at birth or the 3<sup>rd </sup>postnatal day.</p> <p>Conclusions</p> <p>Our findings indicate that the intake of <it>E. Coli </it>by phagocytes is impaired at birth in both preterm and full term neonates compared to adults. This defect is transient, with the phagocytic ability in neonates reaching that of the adults 3 days after birth.</p
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