26 research outputs found
Preparation and characterization of biomimetic silk fibroin/chitosan composite nanofibers by electrospinning for osteoblasts culture
Bacterial cellulose-lactoferrin as an antimicrobial edible packaging
Bacterial cellulose (BC) films from two distinct sources (obtained by static culture with Gluconacetobacter xylinus ATCC 53582 (BC1) and from a commercial source (BC2)) were modified by bovine lactoferrin (bLF) adsorption. The functionalized films (BC+bLF) were assessed as edible antimicrobial packaging, for use in direct contact with highly perishable foods, specifically fresh sausage as a model of meat products. BC+bLF films and sausage casings were characterized regarding their water vapour permeability (WVP), mechanical properties, and bactericidal efficiency against two food pathogens, Escherichia coli and Staphylococcus aureus. Considering their edibility, an in vitro gastrointestinal tract model was used to study the changes occurring in the BC films during passage through the gastrointestinal tract. Moreover, the cytotoxicity of the BC films against 3T3 mouse embryo fibroblasts was evaluated.
BC1 and BC2 showed equivalent density, WVP and maximum tensile strength. The percentage of bactericidal efficiency of BC1 and BC2 with adsorbed bLF (BC1+bLF and BC2+bLF, respectively) in the standalone films and in inoculated fresh sausages, was similar against E. coli (mean reduction 69 % in the films per se versus 94 % in the sausages) and S. aureus (mean reduction 97 % in the films per se versus 36 % in the case sausages). Moreover, the BC1+bLF and BC2+bLF films significantly hindered the specific growth rate of both bacteria. Finally, no relevant cytotoxicity against 3T3 fibroblasts was found for the films before and after the simulated digestion. BC films with adsorbed bLF may constitute an approach in the development of bio-based edible antimicrobial packaging systems.The authors would like to acknowledge Portuguese Foundation
for Science and Technology (Fundação para a Ciência e Tecnologia)
for the research grants: Jorge Padrão SFRH/BD/64901/2009, Sara
Gonçalves SFRH/BD/63578/2009, João Pedro Silva SFRH/BPD/
64958/2009, Ana Cristina Pinheiro SFRH/BPD/101181/2014. V.
Sencadas thanks support from the COST Action MP1206: “Electrospun
nano-fibres for bio inspired composite materials and innovative
industrial applications” and MP1301: “New Generation
Biomimetic and Customized Implants for Bone Engineering”. The
authors would also like to thank the co-funded by the Programa
Operacional Regional do Norte (ON.2 e O Novo Norte), QREN,
FEDER Projects “BioHealth e Biotechnology and Bioengineering
approaches to improve health quality”, Ref. NORTE-07-0124-
FEDER-000027; “BioInd e Biotechnology and Bioengineering for
improved Industrial and Agro-Food processes”, REF. NORTE-07-
0124-FEDER-000028; Strategic Project PEST-C/FIS/UI607/2014;
Matepro eOptimizing Materials and Processes”, ref. NORTE-07-
0124-FEDER-000037; Strategic Project PEst-OE/EQB/LA0023/2013
and project ref. RECI/BBB-EBI/0179/2012 (project number FCOMP-
01-0124-FEDER-027462). Finally, the authors thank the Fundação
para a Ciência e Tecnologia for the strategic funding from the UID/
BIO/04469/2013 unit