Female oxidative status in relation to calcium availability, metal pollution and offspring development in a wild passerine

Both Ca deficiency and metal exposure may affect physiological and nutritional condition of breeding females altering their ability to deposit essential resources (e.g. Ca, antioxidants) into the eggs. This effect of the maternal investment into egg quality is not strictly limited to the embryonic period, but may persist after hatching, since nutrient levels in yolks can compromise nestling antioxidant status, growth and fledging success. The goal of this study was to investigate how metal pollution and Ca availability during the breeding season affect oxidative stress biomarkers and plasma biochemistry in adult female pied flycatchers (Ficedula hypoleuca). In addition, we aim to evaluate how maternal antioxidant status and body condition relate to breeding parameters and offspring oxidative balance. Females breeding in a metal-polluted area in SW Finland showed higher metal concentrations compared to the control area, although current levels were below the toxic level able to affect female physiology. In addition, Ca availability was not constraining female oxidative status and general health in the study area. Interestingly, our results suggested that antioxidant response to metals was better when Ca concentrations were high enough to cover the physiological Ca requirements in breeding females. There seems to be a subtle balance between the concentrations of Ca in the organism and the tolerance to metal-related effects that requires further research. This study supports that offspring oxidative balance and nestling development are affected by maternal body condition and antioxidant status. (C) 2020 Elsevier Ltd. All rights reserved

Bird Feces as Indicators of Metal Pollution: Pitfalls and Solutions

Bird feces are commonly used as a proxy for measuring dietary metal exposure levels in wild populations. Our study aims to improve the reliability and repeatability of fecal metal measurements and gives some recommendations for sampling. First, we studied levels of variation in metallic element (arsenic, calcium, cadmium, cobalt, copper, nickel, lead) concentrations: temporal variation within an individual, among siblings in a brood and among-brood/spatial variation. Second, we explored the variation caused by dual composition (urate vs. feces) of bird droppings. Two sets of fresh fecal samples were collected from pied flycatcher (Ficedula hypoleuca) nestlings living in a metal polluted area in summers 2017 (dataset 1) and 2018 (dataset 2). We found a great deal of temporal intra-individual variation in metal levels, suggesting that dietary exposure varied markedly in a short time scale (within a day). A sample from only one nestling per brood did not well describe the brood mean value, and we recommend that at least four siblings should be sampled. Brood level samples give relatively good temporal repeatability for most metals. For all the metals, the levels in the fecal portion were more than double to those in the urate portion. Since the mass proportion of urate in the bird droppings varied a great deal among samples, standardizing sampling, e.g., by collecting only the fecal part, would markedly reduce the variation due to composition. Alternatively, urate portion could be used for biomonitoring of internally circulated bioavailable metal. View Full-TextKeywords: bird feces; calcium; heavy metals; measurement error; pied flycatcher; repeatability; uric acid</div

Toxic elements in blood of red-necked nightjars (Caprimulgus ruficollis) inhabiting differently polluted environments

Toxic metals have been widely reported in avian tissues due to their well-known accumulation capacity and adverse effects. However, rare earth elements (REE) and other minor elements (ME) are becoming a new threat due to their use in modern technology. Presently, exposure data are limited and no studies have been reported in wildlife. The order Caprimulgiformes is among the most understudied groups of birds lacking blood ecotoxicological data. One major constraint is the small blood sample volume that can be collected to ensure animal welfare, which limits analyses. In order to shed light on these collective issues, we sampled 48 red-necked nightjars (Caprimulgus ruficollis) inhabiting three different scenarios of contaminant exposure (agricultural-urban area, n = 15; mining area, n = 17; and control area, n = 16) in southeastern Spain, and report for the first time concentrations of 50 elements (i.e. trace elements, ATSDR’s list toxic elements, REE and ME) using a recently developed technique able to analyze them by ICP-MS in very small volumes of blood (130 μL). Concentrations of As, Cd, Pb and Mn were significantly higher in individuals captured at the mining area compared to the other sites. Lead levels in the mine site were of particular concern since it was in the range of blood concentrations related to subclinical/clinical effects in other species, and in our study were associated with decreased hematocrit values (up to 44% hematocrit depression at blood concentrations >1000 ng/ml w.w.). Moreover, additive effects related to metal cocktail exposure in the mining area could be expected. Age and gender-related differences in blood concentrations were found for some elements. Even though most REE and ME concentrations were close to the LOQ, some of these emerging contaminants may trigger sublethal effects that, together with the ATSDR’s list toxic elements, need to be carefully evaluated in a future study.</p

Blood toxic elements and effects on plasma vitamins and carotenoids in two wild bird species: Turdus merula and Columba livia

This article belongs to the Special Issue Wildlife Toxicology: An Update on Contaminant Exposure and Effects.Birds have historically suffered adverse effects by toxic elements, such as As, Pb, Hg, and Cd. However, reports on exposure to a wide range of elements, including rare earth elements and other minor elements of emerging concern, and the potential consequences for wildlife are still scarce. This study evaluates blood concentrations of 50 elements and their related effects on lutein and vitamin levels in the Eurasian blackbird (Turdus merula) and wild rock pigeon (Columba livia), inhabiting different scenarios of contaminant exposure. Blood concentrations of As, Cd, and Pb (and Mn in T. merula) were increased in both species captured in the mining area, compared to the control site. T. merula also showed increased As, Cd, and Pb concentrations in blood in the agricultural–urban area, as compared to the control area, together with the highest Hg levels, which could be related to agricultural practices and industrial activities. Decreases of 33 and 38% in the plasma retinol levels in T. merula inhabiting the mining and the agricultural–urban areas, respectively, as compared to the control site, were associated with increased Pb, As, and Cd exposure. This could be duThis research was funded by the Fundación Séneca—Agencia de Ciencia y Tecnología de la Región de Murcia (Saavedra-Fajardo Project 20031/SF/16 to S.Espín). S.Espín was financially supported by Fundación Séneca (20031/SF/16) and by Ministerio de Ciencia, Innovación y Universidades (Juan de la Cierva-Incorporación contract, IJCI-2017-34653). P. Sánchez-Virosta was financially supported by Ministerio de Ciencia, Innovación y Universidades (Juan de la Cierva-Formación contract, FJC2019-042357-I). The analysis of the vitamins was funded by University of Castilla-La Mancha (CGT13-0183). P. R. Camarero benefited from a contract, with reference PTA2017-14583-I, financed by the Spanish Ministry of Economy and Innovation and CSIC. We thank the ERBFacility COST Action (CA16224) for providing an international network for researchers.Peer reviewe

Blood Toxic Elements and Effects on Plasma Vitamins and Carotenoids in Two Wild Bird Species: Turdus merula and Columba livia

Birds have historically suffered adverse effects by toxic elements, such as As, Pb, Hg, and Cd. However, reports on exposure to a wide range of elements, including rare earth elements and other minor elements of emerging concern, and the potential consequences for wildlife are still scarce. This study evaluates blood concentrations of 50 elements and their related effects on lutein and vitamin levels in the Eurasian blackbird (Turdus merula) and wild rock pigeon (Columba livia), inhabiting different scenarios of contaminant exposure. Blood concentrations of As, Cd, and Pb (and Mn in T. merula) were increased in both species captured in the mining area, compared to the control site. T. merula also showed increased As, Cd, and Pb concentrations in blood in the agricultural-urban area, as compared to the control area, together with the highest Hg levels, which could be related to agricultural practices and industrial activities. Decreases of 33 and 38% in the plasma retinol levels in T. merula inhabiting the mining and the agricultural-urban areas, respectively, as compared to the control site, were associated with increased Pb, As, and Cd exposure. This could be due to a metal-driven suppressive effect in retinol metabolism and/or its over-use for coping with metal-related oxidative stress

Blood concentrations of 50 elements in Eagle owl (Bubo bubo) at different contamination scenarios and related effects on plasma vitamin levels

Some metals and metalloids (e.g. Pb, Hg, Cd and As) are well-known for their bioaccumulation capacity and their toxic effects on birds, but concerns on other minor elements and rare earth elements (ME and REE) are growing due to their intensive use in modern technology and potential toxicity. Vitamins and carotenoids play essential roles in nestling growth and proper development, and are known to be affected by the metals classically considered as toxic. However, we are unaware of any attempts to evaluate the exposure to 50 elements and related effects in plasma vitamins and carotenoids in raptor species. The main goals of this study are: (i) to assess the exposure to 50 elements (i.e. classic toxic elements, trace elements, REE and ME) in nestling Eagle owls (Bubo bubo) inhabiting three differently polluted environments (mining, industrial and control areas) in southeastern Spain, and (ii) to evaluate how element exposure affects plasma vitamin and carotenoid levels, hematocrit and body measurements (mass and wing length) of the individuals. Our results show that local contamination in the mining area contributes to increased blood concentrations of Pb, As and Tl in nestlings, while diet differences between control and mining/industrial areas may account for the different levels of Mn, Zn, and Sr in blood, and lutein in plasma. Plasma tocopherol levels were increased in the mining-impacted environment, which may be a mechanism of protection to prevent toxic element-related oxidative stress. Plasma α-tocopherol was enhanced by 20% at blood Pb concentrations ≥8 ng/ml, and nestlings exhibited up to 56% increase in α-tocopherol levels when blood Pb concentrations reached 170 ng/ml. Tocopherol seems to be a sensitive biomarker under an exposure to certain toxic elements (e.g. Pb, As, Tl).Funding was provided by Fundación Séneca - Agencia de Ciencia y Tecnología de la Región de Murcia (20031/SF/16 to S. Espín). S. Espín was supported by Fundación Séneca (20031/SF/16) and by Ministerio de Ciencia, Innovación y Universidades (IJCI-2017-34653). The analysis of vitamins was funded by University of Castilla-La Mancha (CGT13-0183). P. R. Camarero benefited from a contract with reference PTA2017-14583-I financed by the Spanish Ministry of Economy and Innovation and CSIC. We thank the ERBFacility COST Action (CA16224) for providing an international network for researchers.Peer reviewe

Effects of toxic elements on plasma vitamin levels in nestling Eagle owls (Bubo bubo)

Póster presentado al SETAC Europe 31st Annual Meeting, celebrado de forma virtual del 3 al 6 de mayo de 2021.Vitamins and carotenoids play essential roles in nestling growth and proper development, and are known to be affected by toxic metals. The main goal of this communication is to present how element exposure affects plasma vitamin and carotenoid levels in nestling Eagle owls (Bubo bubo) inhabiting three different scenarios of pollution (mining, industrial and control areas) in southeastern Spain. Our results show that local contamination in the mining area contributes to increased blood concentrations of Pb, As and Tl in nestlings, while diet differences between control and mining/industrial areas may account for the different levels of Mn, Zn, and Sr in blood, and lutein in plasma. Plasma tocopherol levels were increased in the mining-impacted environment, which may be a mechanism of protection to prevent toxic-element-related oxidative stress. Plasma α-tocopherol was enhanced by 20 and 56% at blood Pb concentrations ≥8 ng/mL and ≥170 ng/mL, respectively. Tocopherol seems to be a sensitive biomarker under an exposure to certain toxic elements (e.g. Pb, As, Tl).Funding was provided by Fundación Séneca(20031/SF/16) and University of Castilla-La Mancha (CGT13-0183). S. Espín was supported by Fundación Séneca(20031/SF/16) and by Ministerio de Ciencia, Innovación y Universidades (IJCI-2017-34653). P. R. Camarero benefited from a contract financed by the Spanish Ministry of Economy and Innovation and CSIC (PTA2017-14583-I).Peer reviewe