Laparoscopic Distal Pancreatectomy with Splenic Conservation: An Operation without Increased Morbidity

Objectives. The advent of minimally invasive techniques was marked by a paradigm shift towards the use of laparoscopy for benign distal pancreatic masses. Herein we describe one center's experience with laparoscopic distal pancreatectomy. Methods. A retrospective chart review was performed for all distal pancreatectomies completed laparoscopically from 1999 to 2009. Outcomes from those cases completed with a concurrent splenectomy were compared to the spleen-preserving procedures. Results. Twenty-four patients underwent laparoscopic distal pancreatectomy. Seven had spleen-conserving operations. There was no difference in the mean estimated blood loss (316 versus 285 mL, P = .5) or operative time (179 versus 170 minutes, P = .9). The mean tumor size was not significantly different (3.1 versus 2.2 cm, P = .9). There was no difference in the average hospital stay (7.1 versus 7.0 days, P = .7). Complications in the spleen-preserving group included one iatrogenic colon injury, two pancreatic fistulas, and two cases of iatrogenic diabetes. In the splenectomy group, two developed respiratory failure, three acquired iatrogenic diabetes, and two suffered pancreatic fistulas (71% versus 41%, P = .4). Conclusions. The laparoscopic distal pancreatectomy is a safe operation with a low morbidity. Splenic conservation does not significantly increase the morbidity of the procedure

Distribution and natural history of Plutonium zwierleini (Chilopoda: Scolopendromorpha) in Sicily (Italy)

Plutonium zwierleini is a large plutoniumid centipede of great evolutionary interest, occurring with isolated populations along the western Mediterranean area, from Spain to Italy. Due to its rarity and the extreme paucity of available records, P. zwierleini is among the least known Mediterranean chilopods, and scarce information is currently available on its ecology and natural history. Based on an extensive sampling effort carried out in Sicily between 2022 and 2023, we here provide additional occurrence localities for the species across Sicily, and new insights into its ecology. Overall, 29 novel Sicilian records of P. zwierleini, scattered across 21 localities, were collected thus increasing its known Sicilian distribution area by 117%, and the number of localities by 110%. The species was found in a wide range of habitats such as open areas, woods, buildings, and caves, characterizing Plutonium zwierleini as a habitat generalist, whose fine ecological preferenda need to be further explored. Moreover, to explore the diet and behaviour of the species, some specimens were kept in captivity. The captive individuals fed mostly on dead or poorly mobile soft-bodied prey and inert food, without ever displaying predatory behaviour; this suggests that, contrarily to what is currently assumed, P. zwierleini might be a scavenger rather than a predator. The potential distribution of Plutonium zwierleini in Sicily was inferred based on georeferenced occurrence records and climatic variables. The implemented MaxEnt model forecasts the possible occurrence of P. zwierleini on the whole island, with the single exception of its south-easternmost part, possibly due to the local pattern of precipitation seasonality. We hope that the present work might pave the way for further surveys aimed at a better understanding of the ecology of Plutonium zwierleini and the collection of new data in the other regions inhabited by this secretive species

The Grizzly, February 5, 1982

Wismer Thefts Provoke Action • Lloyd\u27s Tenure Rejection Official • KDK Attains Highest GPA • Rod Luck Visits W\u27s Gymnastics • Comment: New Faculty Program a Contradiction of Goals? • On Energy Conservation • Administration\u27s Views on Pledging • Book Store Improves Under New Management • Bell Rate Hikes to Affect Students • Pattern Changes Planned for Next Semester • The Way to a Man\u27s Heart • Japanese Program Opens This Summer • Aquamen Swamp E-town • Racqueteers Rolling • Women Splash to Victory • Sterling Brown to Take Over Football Team • B-Ball Takes Two • Girls Dump Mighty Macshttps://digitalcommons.ursinus.edu/grizzlynews/1071/thumbnail.jp

The Grizzly, January 29, 1982

Two Alumni Join Administration • So Much, POD, in College Bowl Finals • Hermann Eilts on The Middle East • College Receives $500,000 • Teachers Audition For EC/BA Positions • Honor Societies Meet to Discuss Future Plans • Union Undergoes Attractive Renovations • Evening School Expands • Spiritwood Returns for Coffeehouse • German Students Sample American College Life • Mozart Concert to Conclude Winterfest! • Myrin Exhibits Storybook Etchings • Collegeville Claustrophobia? • Schaff Plagued With Repeated Break-ins • Forums, Forums, Forums • Mens Basketball Has Good Vacation • Gymnasts\u27 Work Pays Off • Badminton Opens Season With Temple • Grapplers Impressive in Latest Victories • Hoopsters Lose to Widener • Karas Resigns; New Coach Expected Soon • Widener Drownshttps://digitalcommons.ursinus.edu/grizzlynews/1070/thumbnail.jp

The Grizzly, October 22, 1982

Ferry Named Ursinus Queen • Unique Course Offered • U.C. Choir Presents Bach • Night School Enrollment Up • 1983 Spring Registration • Letters to the Editor • President\u27s Corner • Lewis on Wall Street • Half a Great Show • Watching the Boob Tube • Homecoming \u2782 • And to Prove My Point • New Bus Schedule • Washington Semester: Get Out of Here • Bear Pack Falls to Stiff Competition • Soccer Conquers Albright and Alumni • Grapplers Take to the Mats • Grizzlies Suffer Homecoming Setback • Delaware tops Lady Bearshttps://digitalcommons.ursinus.edu/grizzlynews/1085/thumbnail.jp

Large deletion at the CDC73 gene locus and search for predictive markers of the presence of a CDC73 genetic lesion

The Hyperparathyroidism with Jaw-Tumours syndrome is caused by mutations of the CDC73 gene: it has been suggested that early onset of the disease and high Ca2+ levels may predict the presence of a CDC73 mutation. We searched for large deletions at the CDC73 locus in patients with: HPT-JT (nr 2), atypical adenoma (nr 7) or sporadic parathyroid carcinoma (nr 11) with a specific MLPA and qRT-PCR assays applied on DNA extracted from whole blood. A Medline search in database for all the papers reporting a CDC73 gene mutation, clinical/histological diagnosis, age at onset, Ca2+, PTH levels for familial/sporadic cases was conducted with the aim to possibly identify biochemical/clinical markers predictive, in first diagnosis, of the presence of a CDC73 gene mutation. A novel genomic deletion of the first 10 exons of the CDC73 gene was found in a 3-generation HPT-JT family, confirmed by SNP array analysis. A classification tree built on the published data, showed the highest probability of having a CDC73 mutation in subjects with age at the onset < 41.5 years (44/47 subjects, 93.6%, had the mutation). Whereas the lowest probability was found in subjects with age at the onset ≥ 41.5 years and Ca2+ levels <13.96 mg/dL (7/20 subjects, 35.0%, had the mutation, odds ratio = 27.1, p < 0.001). We report a novel large genomic CDC73 gene deletion identified in an Italian HPT-JT family. Age at onset < 41.5 ys and Ca2+ > 13.96 mg/dL are predictive for the presence of a CDC73 genetic lesion

Diagnostic and prognostic value of B4GALT1 hypermethylation and its clinical significance as a novel circulating cell-free DNA biomarker in colorectal cancer

Epigenetic modifications of glyco-genes have been documented in different types of cancer and are tightly linked to proliferation, invasiveness, metastasis, and drug resistance. This study aims to investigate the diagnostic, prognostic, and therapy-response predictive value of the glyco-gene B4GALT1 in colorectal cancer (CRC) patients. A Kaplan-Meier analysis was conducted in 1418 CRC patients (GEO and TCGA datasets) to assess the prognostic and therapy-response predictive values of the aberrant expression and methylation status of B4GALT1. Quantitative methylation-specific PCR (QMSP) and droplet digital quantitative methylation-specific PCR (dd-QMSP) were respectively used to detect hypermethylated B4GALT1 in metastasis and plasma in four cohorts of metastatic CRC cases (mCRC). Both the downregulated expression and promoter hypermethylation of B4GALT1 have a negative prognostic impact on CRC. Interestingly a low expression level of B4GALT1 was significantly associated with poor cetuximab response (progression-free survival (PFS) p = 0.01) particularly in wild-type (WT)-KRAS patients (p = 0.03). B4GALT1 promoter was aberrantly methylated in liver and lung metastases. The detection of hypermethylated B4GALT1 in plasma of mCRC patients showed a highly discriminative receiver operating characteristic (ROC) curve profile (area under curve (AUC) value 0.750; 95% CI: 0.592-0.908, p = 0.008), clearly distinguishing mCRC patients from healthy controls. Based on an optimal cut-off value defined by the ROC analysis, B4GALT1 yield a 100% specificity and a 50% sensitivity. These data support the potential value of B4GALT1 as an additional novel biomarker for the prediction of cetuximab response, and as a specific and sensitive diagnostic circulating biomarker that can be detected in CRC

An Integrated Meta-Analysis of Two Variants in HOXA1/HOXB1 and Their Effect on the Risk of Autism Spectrum Disorders

BACKGROUND: HOXA1 and HOXB1 have been strongly posed as candidate genes for autism spectrum disorders (ASD) given their important role in the development of hindbrain. The A218G (rs10951154) in HOXA1 and the insertion variant in HOXB1 (nINS/INS, rs72338773) were of special interest for ASD but with inconclusive results. Thus, we conducted a meta-analysis integrating case-control and transmission/disequilibrium test (TDT) studies to clearly discern the effect of these two variants in ASD. METHODS AND FINDINGS: Multiple electronic databases were searched to identify studies assessing the A218G and/or nINS/INS variant in ASD. Data from case-control and TDT studies were analyzed in an allelic model using the Catmap software. A total of 10 and 7 reports were found to be eligible for meta-analyses of A218G and nINS/INS variant, respectively. In overall meta-analysis, the pooled OR for the 218G allele and the INS allele was 0.97 (95% CI = 0.76-1.25, P(heterogeneity) = 0.029) and 1.14 (95% CI = 0.97-1.33, P(heterogeneity) = 0.269), respectively. No significant association was also identified between these two variants and ASD risk in stratified analysis. Further, cumulative meta-analysis in chronologic order showed the inclination toward null-significant association for both variants with continual adding studies. Additionally, although the between-study heterogeneity regarding the A218G is not explained by study design, ethnicity, and sample size, the sensitive analysis indicated the stability of the result. CONCLUSIONS: This meta-analysis suggests the HOXA1 A218G and HOXB1 nINS/INS variants may not contribute significantly to ASD risk

Frequent NRG1 fusions in Caucasian pulmonary mucinous adenocarcinoma predicted by Phospho-ErbB3 expression

NRG1 fusions were recently reported as a new molecular feature of Invasive Mucinous Adenocarcinoma (IMA) of the lung. The NRG1 chimeric ligand acts as a strong inductor of phosphorylation and tyrosine kinase activity of the ErbB2/ErbB3 heterodimer, thus enhancing the PI3K–AKT/MAPK pathways. The NRG1 fusions were widely investigated in Asian IMA cohorts, whereas just anecdotal information are available about the occurrence of NRG1 fusions in IMA Caucasian population. Here we firstly explored a large Caucasian cohort of 51 IMAs and 34 non-IMA cases for the occurrence of NRG1 rearrangements by fluorescent in situ hybridization (FISH) and RNA target sequencing. FISH results were correlated to the immunohistochemical expression of phosphorylated-ErbB3 (pErbB3) receptor and the mutational status of KRAS, EGFR and ALK genes. The NRG1 rearrangements were detected in 31% IMAs and 3% non-IMAs and the CD74-NRG1 fusion transcript variant was characterized in 4 NRG1-positive IMAs. Moreover, pErbB3 expression was found to be strictly associated to the mucinous pattern (p = 0.012, Chi-square test) and all IMA cases showing aberrant expression of pErbB3 demonstrated NRG1 rearrangements. No significant correlation between NRG1 rearrangements and EGFR, KRAS or ALK mutations respectively, was observed. We report for the first time that NRG1 fusions are driver alterations clearly associated with mucinous lung adenocarcinoma subtype of Caucasian patients and not exclusive of Asiatic population. pErbB3 immunostaining may represent a strong predictor of NRG1 fusions, pointing out the detection of pErbB3 by IHC as a rapid and effective pre-screening method to select the NRG1-positive patients