201 research outputs found
Early compaction at day 3 may be a useful additional criterion for embryo transfer
PURPOSE: The reduction of the number of embryos transferred while maintaining a satisfactory rate of pregnancy (PR) with in vitro fertilization calls for a refined technique of embryonic selection. This prospective study investigates the significance of early embryonic compaction at day 3 as a marker of the chances of implantation. METHODS: We examined 317 transfers and their outcome involving 509 embryos including 91 compacted embryos. RESULTS: Early compaction seems linked with the ovarian response to stimulation and embryonic quality. The PR is significantly increased when the embryonic cohort contains at least one compacted embryo (44% versus 29.5%, p = 0.01), and when at least one compacted embryo is transferred (44% versus 31%, p < 0.05). The analysis of our single embryo transfers shows that the implantation rates are significantly better for compacted embryos (50% versus 30%, p < 0.05) (OR 2.98; CI 1.02-5.28). CONCLUSION: Thus, early compaction, sometimes observed at day 3, may serve as a useful additional criterion for selecting the embryos transferred
Are zona pellucida genes involved in recurrent oocyte lysis observed during in vitro fertilization?
PURPOSE: Complete oocyte lysis in in vitro fertilization (IVF) is a rare event, but one against which we remain helpless. The recurrence of this phenomenon in some women in each of their IVF attempts, regardless of treatment, together with the results of animal experiments led us to investigate the possible involvement of the genes encoding for the glycoproteins constituting the zona pellucida (ZP). PATIENTS & METHODS: Over the last ten years, during which we treated over 500 women each year, three women suffered recurrent oocyte lysis during their IVF attempts in our Centre for Reproductive Biology. For each of these three cases, we sequenced the four genes and promoter sequences encoding the glycoproteins of the ZP. The sequence variations likely to cause a change in protein expression or structure, were investigated in a control group of 35 women who underwent IVF without oocyte lysis and with normal rates of fertilization. RESULTS & CONCLUSION: We found no mutations in the ZP genes sequenced. Only some polymorphisms present in the control group and in the general population were detected, excluding their specific involvement in the phenotype observed. Thus, although we suspected that complete oocyte lysis was due to a genetic cause, it did not seem possible to directly incriminate the genes encoding the proteins of the ZP in the observed phenotype. Further study of the genes involved in the processing and organization of ZP glycoproteins may allow elucidation of the mechanism underlying recurrent oocyte lysis during in vitro fertilization
Monte Carlo Dynamics of driven Flux Lines in Disordered Media
We show that the common local Monte Carlo rules used to simulate the motion
of driven flux lines in disordered media cannot capture the interplay between
elasticity and disorder which lies at the heart of these systems. We therefore
discuss a class of generalized Monte Carlo algorithms where an arbitrary number
of line elements may move at the same time. We prove that all these dynamical
rules have the same value of the critical force and possess phase spaces made
up of a single ergodic component. A variant Monte Carlo algorithm allows to
compute the critical force of a sample in a single pass through the system. We
establish dynamical scaling properties and obtain precise values for the
critical force, which is finite even for an unbounded distribution of the
disorder. Extensions to higher dimensions are outlined.Comment: 4 pages, 3 figure
Molecular characterization of corona radiata cells from patients with diminished ovarian reserve using microarray and microfluidic-based gene expression profiling
BACKGROUND: Diminished ovarian reserve (DOR) is one of the causes of infertility in young women. In this prospective study, gene expression profiling (GEP) of corona radiata cells (CRC) was performed to identify genes deregulated in DOR patients. METHODS: Microarray-based GEP of CRC isolated from eight women undergoing IVF was performed to identify genes differentially expressed between patients with normal ovarian reserve and DOR patients. Microfluidic-based quantitative RT-PCR assays were used to validate selected transcripts on 40 independent patients. A principal component analysis was used to identify more homogeneous subgroups of DOR patients. In silico analyses focusing on cis-regulation were performed to refine the interactions between patient\u27s biological characteristics and their GEP. RESULTS: Forty-eight transcripts were differentially expressed, including CXXC finger protein 5 (CXXC5), forkhead box C1 (FOXC1) (down-regulated in DOR) as well as connective tissue growth factor (CTGF), follistatin-like 3 (FSTL3), prostaglandin-endoperoxide synthase 2 (PTGS2) and suppressor of cytokine signaling 2 (SOCS2) (up-regulated in DOR). According to these transcripts, two DOR patients\u27 subgroups (DOR Gr1 and Gr2) were identified. In DOR Gr2 patients, C-terminal domain 2 (CITED2), CTGF, growth arrest-specific 1 (GAS1), insulin receptor substrate 2 (IRS2), PTGS2, SOCS2 and Versican (VCAN) were expressed at significantly higher levels and CXXC5, FOXC1, guanylate-binding protein 2 (GBP2) and zinc finger MIZ-domain containing 1 (ZMIZ1) at significantly lower levels. Higher baseline estradiol (E(2)) levels were observed in DOR Gr2 patients (P < 0.006). The in silico analyses suggested that all 11 genes differentially expressed between DOR Gr1 and DOR Gr2 subgroups could be transcriptional targets of estrogen. CONCLUSIONS: Despite small sample size limitations, 12 genes deregulated in the CRC of DOR patients were identified, which could be involved in DOR pathogenesis. A DOR patient\u27s subgroup with high baseline E(2) levels and deregulated estrogen-responsive genes was also identified
Creep motion in a random-field Ising model
We analyze numerically a moving interface in the random-field Ising model
which is driven by a magnetic field. Without thermal fluctuations the system
displays a depinning phase transition, i.e., the interface is pinned below a
certain critical value of the driving field. For finite temperatures the
interface moves even for driving fields below the critical value. In this
so-called creep regime the dependence of the interface velocity on the
temperature is expected to obey an Arrhenius law. We investigate the details of
this Arrhenius behavior in two and three dimensions and compare our results
with predictions obtained from renormalization group approaches.Comment: 6 pages, 11 figures, accepted for publication in Phys. Rev.
Mitochondrial macro-haplogroup JT may play a protective role in ovarian ageing
This study of 200 Caucasian women shows that the distribution of the mtDNA macro-haplogroups in patients with diminished ovarian reserve (DOR) differed significantly from that of patients with normal ovarian reserve (NOR) (p=0.02). The JT macro-haplogroup was significantly under-represented in DOR patients compared with NOR patients (p=0.006) and compared with the estimated frequency of 18.8% in the general French population (p=0.0012). Our findings suggest that the risk of a prematurely depleted ovarian reserve would be three times lower for patients carrying the JT macro-haplogroup than for patients with any of the other mtDNA haplogroups (odds ratio: 0.3; 95% CI: 0.13-0.74). If these preliminary results are confirmed in larger independent studies, they should lead to the better management of infertility
The depinning transition of a driven interface in the random-field Ising model around the upper critical dimension
We investigate the depinning transition for driven interfaces in the
random-field Ising model for various dimensions. We consider the order
parameter as a function of the control parameter (driving field) and examine
the effect of thermal fluctuations. Although thermal fluctuations drive the
system away from criticality the order parameter obeys a certain scaling law
for sufficiently low temperatures and the corresponding exponents are
determined. Our results suggest that the so-called upper critical dimension of
the depinning transition is five and that the systems belongs to the
universality class of the quenched Edward-Wilkinson equation.Comment: accepted for publication in Phys. Rev.
Roughness at the depinning threshold for a long-range elastic string
In this paper, we compute the roughness exponent zeta of a long-range elastic
string, at the depinning threshold, in a random medium with high precision,
using a numerical method which exploits the analytic structure of the problem
(`no-passing' theorem), but avoids direct simulation of the evolution
equations. This roughness exponent has recently been studied by simulations,
functional renormalization group calculations, and by experiments (fracture of
solids, liquid meniscus in 4He). Our result zeta = 0.390 +/- 0.002 is
significantly larger than what was stated in previous simulations, which were
consistent with a one-loop renormalization group calculation. The data are
furthermore incompatible with the experimental results for crack propagation in
solids and for a 4He contact line on a rough substrate. This implies that the
experiments cannot be described by pure harmonic long-range elasticity in the
quasi-static limit.Comment: 4 pages, 3 figure
Quantum Collective Creep: a Quasiclassical Langevin Equation Approach
The dynamics of an elastic medium driven through a random medium by a small
applied force is investigated in the low-temperature limit where quantum
fluctuations dominate. The motion proceeds via tunneling of segments of the
manifold through barriers whose size grows with decreasing driving force .
In the limit of small drive, at zero-temperature the average velocity has the
form . For strongly
dissipative dynamics, there is a wide range of forces where the dissipation
dominates and the velocity--force characteristics takes the form
, with the
action for a typical tunneling event, the force dependence being determined by
the roughness exponent of the -dimensional manifold. This result
agrees with the one obtained via simple scaling considerations. Surprisingly,
for asymptotically low forces or for the case when the massive dynamics is
dominant, the resulting quantum creep law is {\it not} of the usual form with a
rate proportional to ; rather we find corresponding to and , with the naive scaling exponent for massive
dynamics. Our analysis is based on the quasi-classical Langevin approximation
with a noise obeying the quantum fluctuation--dissipation theorem. The many
space and time scales involved in the dynamics are treated via a functional
renormalization group analysis related to that used previously to treat the
classical dynamics of such systems. Various potential difficulties with these
approaches to the multi-scale dynamics -- both classical and quantum -- are
raised and questions about the validity of the results are discussed.Comment: RevTeX, 30 pages, 8 figures inserte
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