The Reliability and Effectiveness of a Radar-Based Animal Detection System

This document contains data on the reliability and effectiveness of an animal detection system along U.S. Hwy 95 near Bonners Ferry, Idaho. The system uses a Doppler radar to detect large mammals (e.g., deer and elk) when they approach the highway. The system met most of the suggested minimum norms for reliability. The total time the warning signs were activated was at most 90 seconds per hour, and likely substantially less. Animal detection systems are designed to detect an approaching animal. After an animal has been detected, warning signs are activated which allow drivers to respond. Results showed that 58.1–67.9% of deer were detected sufficiently early for northbound drivers, and 70.4–85% of deer were detected sufficiently early for southbound drivers. The effect of the activated warning signs on vehicle speed was greatest when road conditions were challenging (e.g., freezing temperatures and snow- and ice-covered road surface) and when visibility was low (night). In summer, there was no measurable benefit of activated warning signs, at least not as far as vehicle speed is concerned. Depending on the conditions in autumn and winter, the activated warning signs resulted in a speed reduction of 0.69 to 4.43 miles per hour. The report includes practical recommendations for operation and maintenance of the system and suggestions for potential future research

Peer Assisted Learning in Fleximode: Developing an Online Learning Community

Evidence suggests that peer-assisted learning schemes on campus help students establish social networks which can have a positive influence on their learning achievements. At the University of Southern Queensland (USQ), the majority of students are off campus, which raises the urgent question: how to harness the advantages of Meet-Up (formerly PALS: Peer Assisted Learning Strategy) in an online environment? Given that the potential problem of social isolation is even more acute in distance education, how do we develop a peer assisted learning program online which creates a sense of community for its participants? Since 2006, MSN Messenger has been used on a relatively small scale to facilitate this at USQ, with largely positive initial results. Based on evaluations of this initiative, this paper explores the potential of Wimba software, within an institution-wide Moodle learning management system, to extend peer assisted learning programs in a Web 2.0 context

Functional characterisation of Arabidopsis SPL7 conserved protein domains suggests novel regulatory mechanisms in the Cu deficiency response

BACKGROUND: The Arabidopsis SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) transcription factor SPL7 reprograms cellular gene expression to adapt plant growth and cellular metabolism to copper (Cu) limited culture conditions. Plant cells require Cu to maintain essential processes, such as photosynthesis, scavenging reactive oxygen species, cell wall lignification and hormone sensing. More specifically, SPL7 activity promotes a high-affinity Cu-uptake system and optimizes Cu (re-)distribution to essential Cu-proteins by means of specific miRNAs targeting mRNA transcripts for those dispensable. However, the functional mechanism underlying SPL7 activation is still to be elucidated. As SPL7 transcript levels are largely non-responsive to Cu availability, post-translational modification seems an obvious possibility. Previously, it was reported that the SPL7 SBP domain does not bind to DNA in vitro in the presence of Cu ions and that SPL7 interacts with a kin17 domain protein to raise SPL7-target gene expression upon Cu deprivation. Here we report how additional conserved SPL7 protein domains may contribute to the Cu deficiency response in Arabidopsis. RESULTS: Cytological and biochemical approaches confirmed an operative transmembrane domain (TMD) and uncovered a dual localisation of SPL7 between the nucleus and an endomembrane system, most likely the endoplasmic reticulum (ER). This new perspective unveiled a possible link between Cu deficit and ER stress, a metabolic dysfunction found capable of inducing SPL7 targets in an SPL7-dependent manner. Moreover, in vivo protein-protein interaction assays revealed that SPL7 is able to homodimerize, probably mediated by the IRPGC domain. These observations, in combination with the constitutive activation of SPL7 targets, when ectopically expressing the N-terminal part of SPL7 including the SBP domain, shed some light on the mechanisms governing SPL7 function. CONCLUSIONS: Here, we propose a revised model of SPL7 activation and regulation. According to our results, SPL7 would be initially located to endomembranes and activated during ER stress as a result of Cu deficiency. Furthermore, we added the SPL7 dimerization in the presence of Cu ions as an additional regulatory mechanism to modulate the Cu deficiency response. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0231-5) contains supplementary material, which is available to authorized users

A Conserved KIN17 Curved DNA-Binding Domain Protein Assembles with SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 to Adapt Arabidopsis Growth and Development to Limiting Copper Availability

Proper copper (Cu) homeostasis is required by living organisms to maintain essential cellular functions. In the model plant Arabidopsis (Arabidopsis thaliana), the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7) transcription factor participates in reprogramming global gene expression during Cu insufficiency in order to improve the metal uptake and prioritize its distribution to Cu proteins of major importance. As a consequence, spl7 null mutants show morphological and physiological disorders during Cu-limited growth, resulting in lower fresh weight, reduced root elongation, and chlorosis. On the other hand, the Arabidopsis KIN17 homolog belongs to a well-conserved family of essential eukaryotic nuclear proteins known to be stress activated and involved in DNA and possibly RNA metabolism in mammals. In the study presented here, we uncovered that Arabidopsis KIN17 participates in promoting the Cu deficiency response by means of a direct interaction with SPL7. Moreover, the double mutant kin17-1 spl7-2 displays an enhanced Cu-dependent phenotype involving growth arrest, oxidative stress, floral bud abortion, and pollen inviability. Taken together, the data presented here provide evidence for SPL7 and KIN17 protein interaction as a point of convergence in response to both Cu deficiency and oxidative stress

Protecting Spirit of Place: Reconstructing Highways, Maintaining Habitat Connectivity, and Respecting Tribal Culture

The US Highway 93 North reconstruction project on the Flathead Reservation in represents one of the most extensive wildlife-sensitive highway design efforts to date in North America. The reconstruction of the 56 mile long road section included the installation of wildlife crossing structures and wildlife exclusion fences. The mitigation measures were aimed at improving safety for the traveling public through reducing wildlife-vehicle collisions, while simultaneously allowing wildlife to continue to move across the road. These measures were an integral part of the reconstruction of this highway because the Confederated Salish and Kootenai Tribes required the reconstructed highway to be respectful of the land, the people and their culture, and wildlife. This project provided an opportunity to evaluate the extent these mitigation measures helped improve human safety through a reduction in wildlife-vehicle collisions; and maintain habitat connectivity for wildlife.  Wildlife-vehicle collision data were obtained from Montana Department of Transportation.  Completed wildlife crossing structures were monitored for wildlife movements between 2010 and 2015 using wildlife cameras (Reconyx, PM35 and PC900 HyperFire).  The reconstruction of US 93 North improved human safety in general along the entire transportation corridor between Evaro and Polson. The total number of reported crashes decreased by approximately 33%.  However, the number of reported wildlife-vehicle collisions did not decrease over the entire length of the highway between Evaro and Polson. Total wildlife use of the 29 crossing structures that were monitored can be described as substantial with 95,274 successful crossings in total, and 22,648 successful crossings per year