295 research outputs found
Release of Hemoglobin and Potassium from Human Red Blood Cells Treated with Triton X-100 Under the Critical Micellar Concentration
The action of detergents is thought to be connected primarily with micelle formation. However, detergent monomers can also effect biological systems. It was found in this study that human red blood cells can be disintegrated with Triton X-100 non-ionic detergent at a concentration of 0.007 %, lower than the critical micellar concentration (CMC). The time dependent release of hemoglobin and potassium was detected at 37°C and both were sigmoid in character. Although potassium was released faster than hemoglobin, a cooperative relationship between potassium and hemoglobin within the intact red blood cell is suggested by this observation
Synergistic interaction of the cellulosome integrating protein (CipA) from Clostridium thermocellum with a cellulosomal endoglucanase
AbstractActivity of a cellulosomal endoglucanase (endoglucanase E; EGE) from Clostridium thermocellum against two crystalline forms of cellulose was enhanced by combination with the cellulosome integrating protein (CipA), but CipA did not enhance EGE activity against amorphous cellulose, even though it was able to bind to it. Similarly, CipA added in trans to genetically truncated EGE that was unable to combine with it nevertheless enhanced EGE activity against crystalline cellulose. These results indicate that the CipA cellulose binding domain does not mediate an increase in activity solely by bringing the catalytic subunits of the cellulosome complex into intimate contact with the substrate
The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains
Characterization of hybrid proteins consisting of the catalytic domains of Clostridium and Ruminococcus endoglucanases, fused to Pseudomonas non-catalytic cellulose-binding domains
Twisted k-graph algebras associated to Bratteli diagrams
Given a system of coverings of k-graphs, we show that the cohomology of the
resulting (k+1)-graph is isomorphic to that of any one of the k-graphs in the
system. We then consider Bratteli diagrams of 2-graphs whose twisted
C*-algebras are matrix algebras over noncommutative tori. For such systems we
calculate the ordered K-theory and the gauge-invariant semifinite traces of the
resulting 3-graph C*-algebras. We deduce that every simple C*-algebra of this
form is Morita equivalent to the C*-algebra of a rank-2 Bratteli diagram in the
sense of Pask-Raeburn-R{\o}rdam-Sims.Comment: 28 pages, pictures prepared using tik
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