Buoyancy of Atlantic bluefin tuna Thunnus thynnus eggs obtained from captive broodstock spontaneous spawning events

One way to alleviate the pressure on the wild fishery of the Atlantic bluefin tuna (BFT) and aid in its conservation could be its domestication and the development of a self-sustained industry to rear the larvae and produce fingerlings in captive conditions for further grow-out. The Spanish Institute of Oceanography (IEO) is carrying out several research projects on this target for the last 12 years. No one has yet measured the vertical distribution and the in situ buoyancy of bluefin tuna eggs in any of its spawning areas in the world (Mac Kenzie and Mariani, 2012). In the present study the density of bluefin tuna eggs has been measured, comparing it with those of other fish species, particularly Atlantic bonito. We have estimated the speed by which BFT eggs rise to the surface to get a better idea of the potential loss of spawned eggs dragged by the currents out of the cage

Size-selective mortality of laboratory-reared Atlantic bluefin tuna larvae: evidence from microstructure analysis of otoliths during the piscivorous phase

Atlantic bluefin tuna (Thunnus thynnus) larvae show strong piscivorous feeding behavior at the very early larval stage and this enables them to grow at high rates. We conducted a laboratory experiment in which Atlantic bluefin tuna larvae were offered larval prey for the first time at different ages to simulate the early onset of piscivory at three treatments: yolk-sac larvae (YSL), delayed onset of piscivory (DYSL) and a solely planktivorous diet (Rotifers). The otolith microstructure was then used to compare the larval size distribution at the onset of the experiment with the estimated previous size-at-age of the survivors at the end of the experiment by back- calculation. Within a cohort, our results show size-selective mortality of the largest larvae independent of the differences in the timing of onset of piscivory and differences in growth patterns. The results also corroborate the rapid response of Atlantic bluefin tuna to piscivory in terms of growth reflected in the otolith increment widths. Being bigger did not infer a survival advantage and mortality rates did not decline with increasing larval size. Smaller size at a given age could under certain conditions and stages of development confer a survival advantage of individual members of a larval cohort when suitable small-sized prey is available.Versión del editor2,26

Effect of photoperiod and light intensity on larval rearing of bluefin tuna Thunnus thynnus

A couple of trials to test the effects of photoperiod and light intensity on growth and survival of Atlantic bluefin tuna larva were carried out. With regard to light intensity, no significant differences were found between 500, 1000 and 2000 luxes. With regards to photoperiod meanwhile long photoperiods lead to a greater growth, intermediate photoperiods (16hL:8hD and 12hL:12hD ) improve significantly survival rates.Research Project ATAME (CT M2011-29525-C04)

Otolith microstructure analyses in cultured Atlantic bluefin tuna larvae as a tool to provide accurate estimates of size selective growth and mortality

Being bigger is not always the best option to survive and mortality rates do not always decline with increasing larval size. Smaller size at a given age could under certain condition and at certain stages of development confer a survival advantage on individual members of a larval cohort. Reference

ATLANTIC BLUEFIN TUNA (Thunnus thynnus, L.) LARVAE ANTIOXIDANT MOLECULAR FUNCTIONS INDUCED BY DIETARY SELENIUM IN ROTIFER Brachionus rotundiformis

including iodothyronine deiodinases 1, 2 and 3 (dio1, dio2, dio3) was unaffected. Feeding Se enriched rotifers effectively increased Se in ABT larvae tissue. The improved growth observed in Se supplemented treatments might be related to an accelerated development as the flexion index was significantly higher in all Se enriched treatments compared to the non-supplemented control. A similar effect by Se supplementation has been previously described in Senegalese sole (Solea senegalensis) in relation to an enhanced thyroid hormone activity by Se supplementation (Ribeiro et al., 2012). The Se level of 0.10 µg g-1 dw measured in non-supplemented rotifers is below known requirements in fish (Antony Jesu Prabhu et al. 2016). In contrast, rotifers supplemented with the lowest Se level (Se3) contained 4.42 µg Se g-1, which might be sufficient to cover requirements for this mineral as selenoproteins displayed maximum expression in ABT larvae fed this treatment. The increased seleno-enzyme production might have contributed towards an improved antioxidant status in ABT larvae, indicated by a transcriptional downregulation of redox sensitive antioxidant enzymes cat and sod. In conclusion, rotifers without Se enrichment are suboptimal for ABT larvae at first feeding. A dietary Se level of 4.42 µg g-1 dw is recommended as it boosted growth performance and improved the antioxidant status in ABT larvae

Effects of offshore wind farms operational noise on bluefin tuna behaviour

The number of offshore wind farms is growing up quickly in the lasts years. Several studies about its environmental acoustic impacts have been developed at the same time the industry expands, most of them related to the high level impulsive noise produced during the pile diving process associated to the construction stage. Nevertheless, the study of the impact of the operational noise of turbines is very limited. In this paper we investigate the behavioural response of Bluefin tuna when exposed to the operation noise of a turbine. We analysed tuna reaction in terms of three parameters: depth of the school, swimming pattern and changes in the swimming direction. The experiment was developedin a fixed commercial tuna cage in the Mediterranean Sea. The usual behaviour of Bluefin tuna in captivity conditions was previously analysed using a continuous monitorization. Variations in depth were observed when feeding boat approaches, which could be interpreted as a consequence of the acoustical stimulus. The turbine noise was acoustically characterized, and reproduced using a broad-band underwater source. To monitor tuna behaviour two echosounders and a video system were simultaneously used. When exposed to short duration noise tuna behaviour does not exhibit clear disturbances. Nevertheless, with long duration emission tuna reacted: school reduced the radio of thecircular swimming region, moved up to the surface and some individuals were disorientated. Tuna seems to be habituated after several repetitions is short time.Proyecto AZIMUT. This work was financially support from Spanish Government by grant AP2009-4459. We thank both personal and material facilities provided by Grup Balfegó and Nuevo Tomás y Carmen crew

The effect of different short pulse feeding regimes on growth and survival of Atlantic bonito larvae Sarda sarda

One of the most easily manipulated variables in fish larval culture is the photoperiod. Long light photoperiod regimes are commonly used to enhance growth in commercial species. However, for species with a piscivorous larval period, as the Atlantic bonito (Sarda sarda), long time exposure to light could lead to a lower survival through aggressive behavior and cannibalism. One alternative could be modifications of the light and dark cycles during the photophase. These modifications can result in short pulse feeding regimes since bonito larvae fill up their stomach completely during light hours but do not feed in darkness. Little is known about how such intermittent feeding regimes affect growth and survival in fish. In this study, we tried different alternating and continuous light regimes during the culture of bonito larvae to identify the best regime that maximizes growth and survival.Fertilized eggs of Atlantic bonito were obtained from different spontaneous spawning events by a captive broodstock at the Spanish Institute of Oceanography (IEO) in Mazarrón. Bonito larvae 8 days post hatch (dph) were reared in 150 l tanks equipped with a lid that was used to cover and uncover the tanks to manipulate the hours of light and therefore pulse feeding regimes. Bonito larvae were always fed with yolk sac seabream larvae ad libitum. A total of three experiments were conducted. In all, a continuous dark period of 7.5hours was maintained from 24:00 to 7:30. All light regimes had a total of 9 light hours except for one that had 15hours of light (15L:9D). In the first experiment, light regimes provided alternating light and dark conditions of either 1.5, 3 or 4.5hours from 7:30 to 24:00). In the second experiment, the 3hours alternating light regime was compared to two continuous regimes of 15hours of light (15L:9D) and 9hours of light (9L:15D). These two experiments were conducted at the same temperature, 24.7±0.4°C. The third experiment was identical to the second experiment but at colder temperatures, 21.4±0.45°C. All regimes had 3 replicates. 10 larvae were sub-sampled 3 days after the experiments began and were ended after 6 days when all larvae were sampled. Due to slower growth, the third experiment ended after 9 days. The larvae were measured in standard length and individual dry weights were calculated. Larvae were counted in the tanks every 3 days to estimate survivorship. Final larval sizes in the alternating light regimes were larger in the 3hours than those obtained in the 1.5 and 4.5hours (first experiment, Fig. 1a, Tukey test p0.01). The 3hours alternating light regime yielded the largest larval sizes at the end of the experiment compared to the other alternating regimes. Final sizes at the 3hours regime were larger than those obtained under the 9L:15D continuous light regime at both temperatures. The time to satiation and the elapsed time to evacuate food totally from the gut in a similar species is about 3-4hours (Young and Davis, 1990). Our results suggest that a better strategy for bonito larvae growth is to fill their stomach more than once per day followed by a resting period when food is being digested. However, no effect was observed in terms of survival, possibly due to high abundance of larval prey. Changes in the light regime that result in pulse feeding can be a good strategy to increase growth in larval cultures when fitting well the evacuation and satiation rates

The effect of nutritional condition on the growth to post-flexion of bluefin tuna larvae under cultured condition

These findings indicate that protein synthesis increase in proportion to the flexion stage which is strongly associated with the development of the digestive system and therefore the increase in the nutritional condition. Ontogenetic differences in the RNA and DNA content will be discussed in more detail. Also, the pattern obtained for Atlantic bluefin tuna larva will be compared with those of other species

BONE DEVELOPMENT IN ATLANTIC BLUEFIN TUNA Thunnus thynnus AND SKELETAL EFFECTS OF FIRST FEEDING WITH COPEPODS Acartia tonsa OR ROTIFERS Brachionus ibericus

Juvenile production of Atlantic bluefin tuna (Thunnus thynnus) is characterized by high mortalities and low growth rates during the larval stage. Startfeeding of Bluefin tuna larvae in hatcheries depends on the traditional food organisms rotifers and Artemia nauplii (Biswas et al., 2006), and skeletal malformations have been observed in 70% of larvae and juveniles (Libert et al., 2013). When copepods are used as live food, the results are generally improved compared to the use of traditional live feed organisms (Evjemo et al., 2003), with higher survival, increased growth, normal development, earlier onset of ossification and less skeletal anomalies compared to larvae fed rotifers and Artemia (Imsland et al., 2006). The aims of this study were to describe the bone development in the Atlantic Bluefin tuna fed copepods, and to evaluate the effects of start-feeding with enriched rotifers or with cultivated copepods on skeletal deformities