Identifying the Big Questions in paleontology: a community-driven project

Paleontology provides insights into the history of the planet, from the origins of life billions of years ago to the biotic changes of the Recent. The scope of paleontological research is as vast as it is varied, and the field is constantly evolving. In an effort to identify “Big Questions” in paleontology, experts from around the world came together to build a list of priority questions the field can address in the years ahead. The 89 questions presented herein (grouped within 11 themes) represent contributions from nearly 200 international scientists. These questions touch on common themes including biodiversity drivers and patterns, integrating data types across spatiotemporal scales, applying paleontological data to contemporary biodiversity and climate issues, and effectively utilizing innovative methods and technology for new paleontological insights. In addition to these theoretical questions, discussions touch upon structural concerns within the field, advocating for an increased valuation of specimen-based research, protection of natural heritage sites, and the importance of collections infrastructure, along with a stronger emphasis on human diversity, equity, and inclusion. These questions offer a starting point—an initial nucleus of consensus that paleontologists can expand on—for engaging in discussions, securing funding, advocating for museums, and fostering continued growth in shared research directions

G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. <i>thaliana</i>

Heterotrimeric G-proteins are implicated in several plant processes, but the mechanisms of signal-response coupling and the roles of G-protein coupled receptors in general and GCR1 in particular, remain poorly understood. We isolated a knock-out mutant of the Arabidopsis G-protein α subunit (gpa1-5) and analysed its transcriptome to understand the genomewide role of GPA1 and compared it with that of our similar analysis of a GCR1 mutant (Chakraborty et al. 2015, PLoS ONE 10(2):e0117819). We found 394 GPA1-regulated genes spanning 79 biological processes, including biotic and abiotic stresses, development, flavonoid biosynthesis, transcription factors, transporters and nitrate/phosphate responses. Many of them are either unknown or unclaimed explicitly in other published gpa1 mutant transcriptome analyses. A comparison of all known GPA1-regulated genes (including the above 394) with 350 GCR1-regulated genes revealed 114 common genes. This can be best explained by GCR1–GPA1 coupling, or by convergence of their independent signaling pathways. Though the common genes in our GPA1 and GCR1 mutant datasets constitute only 26 % of the GPA1-regulated and 30 % of the GCR1-responsive genes, they belong to nearly half of all the processes affected in both the mutants. Thus, GCR1 and GPA1 regulate not only some common genes, but also different genes belonging to the same processes to achieve similar outcomes. Overall, we validate some known and report many hitherto unknown roles of GPA1 in plants, including agronomically important ones such as biotic stress and nutrient response, and also provide compelling genetic evidence to revisit the role of GCR1 in G-protein signalling

Identifying the Big Questions in paleontology: a community-driven project

Paleontology provides insights into the history of the planet, from the origins of life billions of years ago to the biotic changes of the Recent. The scope of paleontological research is as vast as it is varied, and the field is constantly evolving. In an effort to identify “Big Questions” in paleontology, experts from around the world came together to build a list of priority questions the field can address in the years ahead. The 89 questions presented herein (grouped within 11 themes) represent contributions from nearly 200 international scientists. These questions touch on common themes including biodiversity drivers and patterns, integrating data types across spatiotemporal scales, applying paleontological data to contemporary biodiversity and climate issues, and effectively utilizing innovative methods and technology for new paleontological insights. In addition to these theoretical questions, discussions touch upon structural concerns within the field, advocating for an increased valuation of specimen-based research, protection of natural heritage sites, and the importance of collections infrastructure, along with a stronger emphasis on human diversity, equity, and inclusion. These questions offer a starting point—an initial nucleus of consensus that paleontologists can expand on—for engaging in discussions, securing funding, advocating for museums, and fostering continued growth in shared research directions

Mitigation Approach for Significant Causes of Contractor’s Delays in Construction Projects in Sri Lanka

The construction industry has a dynamic and complex environment. The successful completion of a construction project depends on various factors. Accordingly, construction delay is observed to happen in construction projects. It is highly affecting the construction sector and the economy of the country too. Although previous research studies identified the causes, effects, and mitigation methods separately, this study adopts a linked approach and directs to identify the effective applicable mitigation methods and their practices on each significant cause of delay in construction projects. Moreover, contractors are noted as one of the main sources of construction delay from the previous studies. Hence, this research aims to develop a framework to determine the applicable mitigation strategies and the practices of each mitigation strategy for each significant cause of contractor’s delays in construction projects in Sri Lanka. A mixed research approach was adopted to generate the research process. A literature review was conducted on construction delays, organizational and project management factors, and professional competency, mitigation strategies in delay management. The significant causes of delays and effective mitigation strategies were ranked through a questionnaire survey using a Likert scale. Project Managers and Quantity Surveyors in the Sri Lankan construction industry were selected in the ratio of 1:3 respectively for the interview as they are mostly engaged with delay management. Also, the respondents were limited to 68 and the collected data were analysed through the Relative Important Index (RII) method, to rank the significant causes and mitigation strategies. Then techniques and possible mitigation strategies were found through interviews and content analysis was used to analyse the data. The findings indicated that most of the contractor’s delays can be mitigated through applying proper mitigation strategies as effective management approaches in the construction projects such as effective project planning and schedules of construction works, maintain positive financial stability and well qualified and experienced professionals and staff, effective strategic planning, risk analysis, and management, selection of suitable sub-contractor and management and effective site management and supervision. In addition to that, inaccurate planning and scheduling of the project, financial dishonesty, poor procurement of construction materials, ineffective time management of contractors, and incompetence of management professionals and staffs were identified as the most significant contractor’s causes of delays in construction management. Keywords: Delays, Significant Causes, Mitigation, Construction Management, Construction Projects

Mitigation Approach for Significant Causes of Contractor’s Delays in Construction Projects in Sri Lanka

The construction industry has a dynamic and complex environment. The successful completion of a construction project depends on various factors. Accordingly, construction delay is observed to happen in construction projects. It is highly affecting the construction sector and the economy of the country too. Although previous research studies identified the causes, effects, and mitigation methods separately, this study adopts a linked approach and directs to identify the effective applicable mitigation methods and their practices on each significant cause of delay in construction projects. Moreover, contractors are noted as one of the main sources of construction delay from the previous studies. Hence, this research aims to develop a framework to determine the applicable mitigation strategies and the practices of each mitigation strategy for each significant cause of contractor’s delays in construction projects in Sri Lanka. A mixed research approach was adopted to generate the research process. A literature review was conducted on construction delays, organizational and project management factors, and professional competency, mitigation strategies in delay management. The significant causes of delays and effective mitigation strategies were ranked through a questionnaire survey using a Likert scale. Project Managers and Quantity Surveyors in the Sri Lankan construction industry were selected in the ratio of 1:3 respectively for the interview as they are mostly engaged with delay management. Also, the respondents were limited to 68 and the collected data were analysed through the Relative Important Index (RII) method, to rank the significant causes and mitigation strategies. Then techniques and possible mitigation strategies were found through interviews and content analysis was used to analyse the data. The findings indicated that most of the contractor’s delays can be mitigated through applying proper mitigation strategies as effective management approaches in the construction projects such as effective project planning and schedules of construction works, maintain positive financial stability and well qualified and experienced professionals and staff, effective strategic planning, risk analysis, and management, selection of suitable sub-contractor and management and effective site management and supervision. In addition to that, inaccurate planning and scheduling of the project, financial dishonesty, poor procurement of construction materials, ineffective time management of contractors, and incompetence of management professionals and staffs were identified as the most significant contractor’s causes of delays in construction management.&#x0D; Keywords: Delays, Significant Causes, Mitigation, Construction Management, Construction Projects.</jats:p

Prevalence of biofilm and beta-lactamase producing Staphylococcus in nasal and throat isolates from healthy volunteers: A medical alert

Background: The Staphylococci species are the predominant inhabitants of the nose and throat as normal flora, they are now becoming a threat due to their capacity to produce biofilm. Biofilm related infections are difficult to be treated unless it is diagnosed early. The aim was to study the prevalence of biofilm and beta-lactamase production in Staphylococcus isolates from nasal and throat mucosa in healthy volunteers.Methods: Staphlococcal isolates were collected from nasal and throat swabs of 100 healthy volunteers at Gulf Medical University and Gulf Medical College Hospital, Ajman, UAE. Staphylococcus aureus or Coagulase Negative Staphylococcus (CoNS) was classified from growth on Mannitol Salt Agar and tube coagulase test. Biofilm production detected by Christensen’s tissue culture plate and Congo red agar methods. Betalactamase production among biofilm producers identified by iodometric tube method.Results: Among 100 Staphylococcus isolates, 41 were Staphylococcus aureus of which 61% were biofilm producers of which 46.3% were betalactamase positive. Among the 59 CoNS isolates, 64.4% were biofilm producers of which 30.5% were beta-lactamase positive. Predominance of nasal Staphylococcal isolation observed among males at the age group of &lt;20 years.Conclusion: Biofilm-producing Staphylococcus inhabits the nasal and throat mucosa of healthy individuals. Beta-lactamase production was higher in Staphylococcus aureus positive for biofilm producers as compared to CoNS. Transmission of these biofilm producers with drug resistance factors from the healthy individuals to high risk patients with indwelling devices need to be considered.Nepal Journal of Medical Sciences Vol.3(2) 2014: 79-83</jats:p

An investigation into value addition concept correlated to facilities management

The purpose of this research is to enable Facilities Management (FM) decision makers to identify key FM interventions that add value to the organisations and to manage a successful implementation and to measure the outputs. This study inaugurated with literature review, and then a preliminary survey was carried out to validate the data gathered from the literature review. To inform the findings reported in this paper data was collected through semi structured interviews with expert from different industrial backgrounds. Empirical finding shows that most industrial professions think they should apply the concept of Adding Value in daily practice but there are constraints such as resistance from top management, limitations within the hierarchy, workload factor etc. Many experts identified that identifying a particular added value and the part FM played in is extremely complex and momentarily difficult. And the most acceptable interventions which were identified through interviews are changing the physical environment, changing the facilities services and strategic advice and planning. All the interviewees agreed that they only use Key Performance Indicators (KPIs) to measure the performance of facility related activities