Computational workflow for investigating highly variable genes in single-cell RNA-seq across multiple time points and cell types.

Here, we present a computational approach for investigating highly variable genes (HVGs) associated with biological pathways of interest, across multiple time points and cell types in single-cell RNA-sequencing (scRNA-seq) data. Using public dengue virus and COVID-19 datasets, we describe steps for using the framework to characterize the dynamic expression levels of HVGs related to common and cell-type-specific biological pathways over multiple immune cell types. For complete details on the use and execution of this protocol, please refer to Arora et al.1

Single-cell temporal analysis of natural dengue infection reveals skin-homing lymphocyte expansion one day before defervescence.

Effective clinical management of acute dengue virus (DENV) infection relies on the timing of suitable treatments during the disease progression. We analyzed single-cell transcriptomic profiles of the peripheral blood mononuclear cell samples from two DENV patients, collected daily during acute phase and also at convalescence. Key immune cell types demonstrated different dynamic responses over the course of the infection. On the day before defervescence (Day -1), we observed the peak expression of several prominent genes in the adaptive immunological pathways. We also characterized unique effector T cell clusters that expressed skin-homing signature genes at Day -1, whereas upregulation of skin and gut homing genes was also observed in plasma cells and plasmablasts during the febrile period. This work provides an overview of unique molecular dynamics that signify the entry of the critical phase, and the findings could improve the patient management of DENV infection

Common Fixed Point and Coupled Coincidence Point Theorems for Geraghty’s Type Contraction Mapping with Two Metrics Endowed with a Directed Graph

The purpose of this paper is to present some existence and uniqueness results for common fixed point theorems for θ-ϕ contraction mappings with two metrics endowed with a directed graph. In addition, by using our main results, we obtain some results about coupled coincidence points endowed with a directed graph. Our results generalize those presented in previous papers

Fixed Point Theorems for Multivalued Nonself G-Almost Contractions in Banach Spaces Endowed with Graphs

In this paper, we prove some fixed point theorems for multivalued nonself G-almost contractions in Banach spaces with a directed graph and give some examples to illustrate our main results. The main results in this paper extend and generalize many known results in the literature therein