3D Visualization and Measurement of Capillaries Supplying Metabolically Different Fiber Types in the Rat Extensor Digitorum Longus Muscle During Denervation and Reinnervation

The aim of this study was to determine whether capillarity in the denervated and reinnervated rat extensor digitorum longus muscle (EDL) is scaled by muscle fiber oxidative potential. We visualized capillaries adjacent to a metabolically defined fiber type and estimated capillarity of fibers with very high oxidative potential (O) vs fibers with very low oxidative potential (G). Capillaries and muscle fiber types were shown by a combined triple immunofluorescent technique and the histochemical method for NADH-tetrazolium reductase. Stacks of images were captured by a confocal microscope. Applying the Ellipse program, fibers were outlined, and the diameter, perimeter, cross-sectional area, length, surface area, and volume within the stack were calculated for both fiber types. Using the Tracer plug-in module, capillaries were traced within the three-dimensional (3D) volume, the length of capillaries adjacent to individual muscle fibers was measured, and the capillary length per fiber length (Lcap/Lfib), surface area (Lcap/Sfib), and volume (Lcap/Vfib) were calculated. Furthermore, capillaries and fibers of both types were visualized in 3D. In all experimental groups, O and G fibers significantly differed in girth, Lcap/Sfib, and Lcap/Vfib, but not in Lcap/Lfib. We conclude that capillarity in the EDL is scaled by muscle fiber size and not by muscle fiber oxidative potential. (J Histochem Cytochem 57:437–447, 2009

The 2003 Slovenian Alcohol Policy: Background, Supporters, and Opponents

High levels of wine, beer, and spirits consumption have historically characterized Slovenian drinking culture. The geographical location of the country provides an ideal environment for wine production, historical ties with the Austro-Hungarian Empire contribute to the tradition of beer drinking, and the custom of home distilling has resulted in a considerable level of spirits consumption. This combination of factors contributes to the high level and cultural acceptance of alcohol consumption in Slovenia. Alcohol-related harm in Slovenia was recognized as a problem as early as 1834, and since then Slovenian public health experts have implemented various programs and policies in an attempt to reduce alcohol-related harm and alcohol consumption in general. A report published by Slovenian public health experts and presented to the Slovenian Ministry of Health in the late 1990s showed that alcohol consumption and related harm were among the highest in the European region, and that there was a need for a policy to reduce consumption and alcohol-related harm in the country. This article outlines the events leading up to this policy. Although business interests and some politicians and public health experts opposed the policy, it was passed by the Slovenian National Assembly on January 28, 2003. Descriptive data revealing a subsequent decrease in the rate of registered alcohol consumption and in deaths due to liver disease, cirrhosis, and suicide may represent preliminary indicators of the effective implementation of the Act