4 research outputs found
Additional file 1 of The Prospective Lynch Syndrome Database: background, design, main results and complete MySQL code
Additional file 1
Additional file 1: of Identification of genetic variants for clinical management of familial colorectal tumors
Table S1. Primers used in the pCAS2 minigene splicing assay. (DOCX 15Â kb
Additional file 1: of Genetic variants of prospectively demonstrated phenocopies in BRCA1/2 kindreds
The concentration in a 10 ml PCR was 1xThermopol Reaction Buffer with 2 mM MgS04, 0.3 μM “reverse” primers, 0.15 μM “forward” primer, 0.1 μM, 6-Carboxyfluorescein-GC clamp primer, 600 μM dNTP, 100 μg Bovine Serum Albumine (Sigma-Aldrich, Oslo, Norway) and 0.75 U Taq DNA polymerase. Plates were sealed with two strips of electrical tape (Clas Ohlson, Oslo, Norway). The temperature cycling was repeated 35 times; 94 °C for 30 s, annealing temperature held for 30 s and extension at 72 °C for 60 s (Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany)). Table S1. primers used to amplify PCR product to be analysed by cycling temperature capillary electrophoresis. (DOCX 16 kb