Functionality of natural killer cells in obese asthma phenotypes

© 2022 John Wiley & Sons Ltd.Background: Obesity-associated asthma (OA) is a difficult to treat asthma phenotype due to its severity and poor response to inhaled steroids. Early-onset allergic (EoOA) and late-onset non-allergic (LoOA) OA are suggested subtypes of this phenotype. Natural Killer (NK) cells are key elements of innate immunity involved in cytotoxicity and immune regulation, with uncertain role in OA pathogenesis. Methods: Early-onset allergic and LoOA patients together with obese non-asthmatic (ONA) controls have been enrolled in the study. Peripheral blood samples have been collected for analysis. Percentages of total NK cells, CD3−CD56dim and CD3−CD56bright NK cell subsets, cytotoxic activity, intracellular interferon-γ, interleukin (IL)-10, IL-13, IL-17 secretion and activatory receptors (NKG2D, NKp46i and NKp44) have been investigated by flow cytometry. The effect of IL-12 and IL-23 stimulation on NK cells and intracellular cytokines in different groups have also been analysed and compared with unstimulated conditions. Results: Results of ONA (n = 5, age 42 ± 8), EoOA (n = 5, age 42 ± 10) and LoOA (n = 8, age 46 ± 8) patients have analysed. Body Mass Index has been found to be negatively correlated with CD69 (p =.022, r = −0.534). NKG2D receptor has been significantly low in CD56dim cells of asthma population (p =.046). NKp44 receptor expression has increased after IL-12 stimulation in EoOA and control group (p =.02). Intracellular IL-10 content has increased in LoOA and control subjects (p =.018, p =.03) but not in the EoOA group. Intracellular IL-17 level has found be higher in allergic OA group. LoOA patients showed a decreased NK cytotoxicity compared with the early-onset asthma group (p =.05). Conclusion: Our study suggests an impaired NK receptor expression, activation and reduced cytotoxicity in OA patients together with variances between different subtypes of this phenotype. This data would be beneficial for tailoring a more personalized treatment strategy combatting steroid resistance and frequent exacerbations in this group of patients

The Reliability and Validation of the Turkish Version of the Asthma Self-Management Knowledge Questionnaire

OBJECTIVES: There is no validated questionnaire in Turkish to assess asthma knowledge. In this study, we aimed to evaluate the reliability and validity of the Turkish version of the Asthma Self-Management Knowledge Questionnaire (AKQ) among asthmatic adults

Pulmonary thromboendarterectomy: Case Report

Thromboendarterectomy is one of the choices for the treatment of chronic pulmonary hypertension related to chronic thromboembolic obstruction. Thromboendarterectomy can be life-saving for patients who have homodynamic instability despite active and appropriate medical treatment, who have massive pulmonary obstruction at one of the main pulmonary artery and who are young and without any additional pulmonary and cardiac disease. Although the mortality rate is about 10% in reported studies, it can be decreased by suitable patient selection, experience and good postoperative intensive care. Thromboendarterectomy experience is very limited in Turkey because of the lack of experience, high mortality rate and lack of multidisciplinary approach. In this paper, a chronic pulmonary thromboembolism case who is treated with surgical thromboendarterectomy is presented to aid experience about this subject in our country

The role of atopy in the pathogenesis of bleomycin pulmonary toxicity.

Introduction: Bleomycin pulmonary toxicity (BPT) is a potentially life-threatening consequence of bleomycin usage in patients. An overproduction of epithelium-derived cytokines, habitually linked to allergic inflammation, has been recently revealed in experimental models of BPT

Effect of drug desensitization on drug hypersensitivity-related quality of life

[No Abstract Available