DETERMINATION OF PRL, PROP1 AND GH GENE POLYMORPHISMS IN INDIGENOUS GOAT BREEDS IN TURKEY

It is predicted that yield characteristics will be affected in the negative direction as a result of future global warming. Genotyping gene regions affecting the yield characteristics of animals today and thereby contributing to minimizing this situation in which producers can be affected in the negative direction. The aim of this study is to determine genotype and allele distribution of PRL, GH and PROP1 genes of the indigenous goat breeds in Turkey. Unrelated 50 Anatolian Black, 50 Kilis and 50 Angora goat breeds are used as the sample of the study. The genotype and allele frequencies of PRL and PROPI were detected by using polymerase chain reaction-restriction fragment length polymorphism method. The GH polymorphism was determined by direct sequencing. C allele frequency of PROP1 and A allele frequency of the PRL was found to be high in Anatolian Black, Kilis and Angora goat breeds. Only BB genotype was observed in 5' promoter region GH gene. As a result, it can be suggested that these three genes need further investigation on more goat breeds and larger numbers of individuals and also further investigations should be carried out in order to understand the relations between gene and efficiency traits. Based on these, future studies on Anatolian breeds can lead to more definitive results

DETERMINATION OF PRL, PROP1 AND GH GENE POLYMORPHISMS IN INDIGENOUS GOAT BREEDS IN TURKEY

WOS: 000452689400091It is predicted that yield characteristics will be affected in the negative direction as a result of future global warming. Genotyping gene regions affecting the yield characteristics of animals today and thereby contributing to minimizing this situation in which producers can be affected in the negative direction. The aim of this study is to determine genotype and allele distribution of PRL, GH and PROP1 genes of the indigenous goat breeds in Turkey. Unrelated 50 Anatolian Black, 50 Kilis and 50 Angora goat breeds are used as the sample of the study. The genotype and allele frequencies of PRL and PROPI were detected by using polymerase chain reaction-restriction fragment length polymorphism method. The GH polymorphism was determined by direct sequencing. C allele frequency of PROP1 and A allele frequency of the PRL was found to be high in Anatolian Black, Kilis and Angora goat breeds. Only BB genotype was observed in 5' promoter region GH gene. As a result, it can be suggested that these three genes need further investigation on more goat breeds and larger numbers of individuals and also further investigations should be carried out in order to understand the relations between gene and efficiency traits. Based on these, future studies on Anatolian breeds can lead to more definitive results.Istanbul University Scientific Research Projects UnitIstanbul University [28122, 56717, 51900]This project was supported by Istanbul University Scientific Research Projects Unit, Project no: 28122, 56717 and 51900

Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep

Kivircik sheep is an important local Turkish sheep according to its meat quality and milk productivity. The aim of this study was to analyze gene expression profiles of both prenatal and postnatal stages for the Kivircik sheep. Therefore, two different cDNA libraries, which were taken from the same Kivircik sheep mammary gland tissue at prenatal and postnatal stages, were constructed. Total 3072 colonies which were randomly selected from the two libraries were sequenced for developing a sheep ESTs collection. We used Phred/Phrap computer programs for analysis of the raw EST and readable EST sequences were assembled with the CAP3 software. Putative functions of all unique sequences and statistical analysis were determined by Geneious software. Total 422 ESTs have over 80% similarity to known sequences of other organisms in NCBI classified by Panther database for the Gene Ontology (GO) category. By comparing gene expression profiles, we observed some putative genes that may be relative to reproductive performance or play important roles in milk synthesis and secretion. A total of 2414 ESTs have been deposited to the NCBI GenBank database (GW996847-GW999260). EST data in this study have provided a new source of information to functional genome studies of sheep

Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep

WOS: 000394141500001PubMed ID: 28239610Kivircik sheep is an important local Turkish sheep according to its meat quality and milk productivity. The aim of this study was to analyze gene expression profiles of both prenatal and postnatal stages for the Kivircik sheep. Therefore, two different cDNA libraries, which were taken from the same Kivircik sheep mammary gland tissue at prenatal and postnatal stages, were constructed. Total 3072 colonies which were randomly selected from the two libraries were sequenced for developing a sheep ESTs collection. We used Phred/Phrap computer programs for analysis of the raw EST and readable EST sequences were assembled with the CAP3 software. Putative functions of all unique sequences and statistical analysis were determined by Geneious software. Total 422 ESTs have over 80% similarity to known sequences of other organisms in NCBI classified by Panther database for the Gene Ontology (GO) category. By comparing gene expression profiles, we observed some putative genes that may be relative to reproductive performance or play important roles in milk synthesis and secretion. A total of 2414 ESTs have been deposited to the NCBI GenBank database (GW996847-GW999260). EST data in this study have provided a new source of information to functional genome studies of sheep.Yildiz Technical University Scientific Research Projects Coordination DepartmentYildiz Technical University [27-01-07-02]This research has been supported by Yildiz Technical University Scientific Research Projects Coordination Department (Project no. 27-01-07-02)

Indel mutation of the ADD1/SREBP-1c gene in the South Anatolian Red and East Anatolian Red cattle breeds

The present study was designed to determine genotypes of adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. Fifty cattle from the SAR breed and 43 cattle from the EAR breed were used. Intron 7 of the ADD1/SREBP1c gene containing an 84 bp indel mutation was amplified by polymerase chain reaction (PCR). The number of SS genotyped individuals with the 84 bp deletion associated with fatty acid composition and growth traits were very low in both breeds. The frequency of the LL genotype with the 84 bp insertion was significantly higher. The 2 cattle populations were in Hardy-Weinberg equilibrium. The SS genotype was observed in the SAR and EAR breeds. As a result, we can conclude that determination of ADD1/SREBP1c genotype distribution in a great number of cattle breeds of Bos taurus and Bos indicus would allow us to observe more SS genotypes

Analysis of Expressed Genes in Normal and Tumoral Mammary Gland Tissue of the Terrier Dog

WOS: 000429197900005Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in the dog could be a model for human mammary gland tumors. In this study, we constructed two different cDNA libraries from mammary tissue, which were collected from a normal mammary tissue of a healthy Terrier dog and a tumoral mammary tissue of a sick dog. A total 2304 colonies which are randomly picked out from the two libraries were sequenced for developing a dog mammary gland ESTs collection. Raw EST data were analyzed with Phred/Phrap programs and readable EST sequences were assembled with the CAP3 program. All of EST sequences were grouped into 45 contig and 2203 singletons. Putative functions of all unique sequences were designated by NCBI BLAST based on gene homology and annotated by BLAST2GO. The results of this study are a very valuable resource for functional genome studies of the dogs