360 research outputs found
Antioxidant effects of antioxidant biofactor on reactive oxygen species in human gingival fibroblasts
The purpose of this study was to investigate the effects of antioxidant biofactor (AOB) on reactive oxygen species (ROS). Generation of superoxide radical (O2•−) and hydroxyl radical (•OH) was determined using an electron spin resonance (ESR) spin-trapping method. AOB was added at different concentrations to these free radical generating systems. The generation of both O2•− and •OH was scavenged by the addition of AOB in a dose-dependent manner. These results indicate that AOB has strong antioxidant properties against these radicals. We further investigated the anti-oxidative effect of AOB on human gingival fibroblasts (HGFs). HGFs were treated for 3 h with α-MEM containing a combination of AOB and H2O2 (AOB + H2O2 group), containing H2O2 (H2O2 group), or containing AOB alone (AOB group). Non-stimulated HGFs were used as a control group. The number of surviving cells was in the order of the AOB group > control group > AOB + H2O2 group > H2O2 group. The level of expression of type I collagen mRNA and production of collagen were also in the order of the AOB group > control group > AOB + H2O2 group > H2O2 group. In conclusion, our results suggest that AOB may protect HGFs against oxidative stress by reducing stress-induced ROS
Phasons Formed on Si(100) Dimer Rows Observed by Scanning Tunneling Microscopy(STM-Si(100))
At~6K, dimers on Si(100) surface are buckled, and structural change occurs between c(4x2) and p(2x2) arrangements due to dimer flip-flop motion at phase boundaries on dimer rows. The phase defect appearing at boundaries has a structure similar to that of the type-C defect, two adjacent dimers are buckled in the same orientation. In consideration of the dimer arrangement around the phase boundary, there exist structures with two different conformations for the phase defect, however, according to the Ising spin model, both of which have the same energy higher compared to other buckled dimers with 2x anticorrelation along a dimer row. Therefore, dimer flip-flop motion at a phase boundary results in the migration of a solitary phase defect with higher energy, as a phason
Effect of Paraquat-Induced Oxidative Stress on Insulin Regulation of Insulin-Like Growth Factor-Binding Protein-1 Gene Expression
Oxidative stress is thought to play a role in the development of insulin resistance. In order to elucidate the molecular effect of oxidative stress on liver insulin signaling, we analyzed the effect of paraquat (1,1-dimethyl-4,4-dipyridynium; PQ)-derived oxidative stress on the expression of insulin-dependent genes and activation of liver insulin signaling pathway. Incubation of primary cultured rat hepatocytes with 2 mM PQ for 6 h impaired the suppressive effect of insulin on insulin-like growth factor-binding protein-1 (IGFBP-1) gene expression, but did not influence glucose-6-phosphatase gene expression. Insulin-dependent phosphorylation or activation of insulin receptor, insulin receptor substrate-1 and -2, phosphatidylinositol 3-kinase, Akt and forkhead in rhabdomyosarcoma were not affected by PQ pre-treatment. In contrast, PQ treatment impaired insulin-dependent phosphorylation of mammalian target of rapamycin (mTOR). These results indicate that PQ-induced oxidative stress impairs insulin-dependent mTOR activation and that this impairment probably causes inhibition of insulin-dependent repression of IGFBP-1 expression
MAXI GSC observations of a spectral state transition in the black hole candidate XTE J1752-223
We present the first results on the black hole candidate XTE J1752-223 from
the Gas Slit Camera (GSC) on-board the Monitor of All-sky X-ray Image (MAXI) on
the International Space Station. Including the onset of the outburst reported
by the Proportional Counter Array on-board the Rossi X-ray Timing Explorer on
2009 October 23, the MAXI/GSC has been monitoring this source approximately 10
times per day with a high sensitivity in the 2-20 keV band. XTE J1752-223 was
initially in the low/hard state during the first 3 months. An anti-correlated
behavior between the 2-4 keV and 4-20 keV bands were observed around January
20, 2010, indicating that the source exhibited the spectral transition to the
high/soft state. A transient radio jet may have been ejected when the source
was in the intermediate state where the spectrum was roughly explained by a
power-law with a photon index of 2.5-3.0. The unusually long period in the
initial low/hard state implies a slow variation in the mass accretion rate, and
the dramatic soft X-ray increase may be explained by a sudden appearance of the
accretion disk component with a relatively low innermost temperature (0.4-0.7
keV). Such a low temperature might suggest that the maximum accretion rate was
just above the critical gas evaporation rate required for the state transition.Comment: Publication of Astronomical Society of Japan Vol.62, No.5 (2010) [in
print
Bright X-ray flares from the BL Lac object Mrk 421, detected with MAXI in 2010 January and February
Strong X-ray flares from the blazar Mrk 421 were detected in 2010 January and
February through the 7 month monitoring with the MAXI GSC. The maximum 2 -- 10
keV flux in the January and February flares was measured as 120 +- 10 mCrab and
164 +- 17 mCrab respectively; the latter is the highest among those reported
from the object. A comparison of the MAXI and Swift BAT data suggests a convex
X-ray spectrum with an approximated photon index of about 2. This spectrum is
consistent with a picture that MAXI is observing near the synchrotron peak
frequency. The source exhibited a spectral variation during these flares,
slightly different from those in the previous observations, in which the
positive correlation between the flux and hardness was widely reported. By
equating the halving decay timescale in the January flare, s, to the synchrotron cooling time, the magnetic field was
evaluated as B = 0.045 G , where is the jet
beaming factor. Assuming that the light crossing time of the emission region is
shorter than the doubling rise time, s,
the region size was roughly estimated as cm
. These are consistent with the values previously reported. For
the February flare, the rise time, s, gives a
loose upper limit on the size as cm ,
although the longer decay time s, indicates
B = 0.015 G , which is weaker than the previous results.
This could be reconciled by invoking a scenario that this flare is a
superposition of unresolved events with a shorter timescale.Comment: 14 pages, 4 figures, accepted for PASJ (Vol. 62 No. 6
A Large X-ray Flare from a Single Weak-lined T Tauri Star TWA-7 Detected with MAXI GSC
We present a large X-ray flare from a nearby weak-lined T Tauri star TWA-7
detected with the Gas Slit Camera (GSC) on the Monitor of All-sky X-ray Image
(MAXI). The GSC captured X-ray flaring from TWA-7 with a flux of
ergs cm s in 2--20 keV band during the scan
transit starting at UT 2010-09-07 18:24:30.The estimated X-ray luminosity at
the scan in the energy band is 3 ergs s,indicating that
the event is among the largest X-ray flares fromT Tauri stars.Since MAXI GSC
monitors a target only during a scan transit of about a minute per 92 min
orbital cycle, the luminosity at the flare peak might have been higher than
that detected. At the scan transit, we observed a high X-ray-to-bolometric
luminosity ratio, log = ; i.e., the
X-ray luminosity is comparable to the bolometric luminosity. Since TWA-7 has
neither an accreting disk nor a binary companion, the observed event implies
that none of those are essential to generate such big flares in T Tauri stars.Comment: 4 pages, 2 figures, 1 table accepted for publication in PAS
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