Morte embrionária precoce em éguas: aspectos clínicos e hormonais

The present study aimed to diagnose early embryonic death in 128 mares. Blood samples were collected at the 4th, 7th, 10th, 13th, 16th, 19th, 21st and 30th day after ovulation and the diameter of the corpus luteum was measured. The diameter and characteristics of the embryonic vesicle were evaluated by ultrasonography. Microbiology, cytology and histopathology were carried out in mares with embryonic death. Plasma oestrogen and progesterone concentration were measured by radioimmunoassay. From 128 mares, 17 (13.28%) showed early embryonic death. The embryonic losses took place at 19 days of pregnancy in 47.05% and at 21 days of pregnancy in 29.4% of the mares. The diameter of the corpus luteum in the mares that maintained pregnancy was similar to those with embryonic loss. Otherwise, the diameter of the embryonic vesicles was bigger at the 16th, 19th, 21st and 30th day of pregnancy when compared to the mares with embryonic loss. The mean plasma progesterone concentration was similar in both groups and the median plasma oestrogen concentration was higher in the pregnant mares. The cytological, microbiological and histopathological exams revealed that most of the mares had endometritis. Ultrasonography provided an early diagnosis of pregnancy (from the 12th day post-ovulation) and important information about the development of embryo and embryonic death. Endometritis was considered the main cause of embryonic losses in this study.O presente estudo objetivou diagnosticar as causas da morte embrionária precoce em 128 éguas. Amostras de soro foram coletadas nos dias 4, 7, 10, 13, 16, 19, 21 e 30 após ovulação, para dosagens hormonais e mensurações do corpo lúteo. O diâmetro e as características da vesícula embrionária foram avaliados, através da ultra-sonografia, a partir do 12º dia pós-ovulação. Das 128 éguas estudadas, 17 (13.28%) apresentaram morte embrionária. O diâmetro do corpo lúteo, bem como as concentrações plasmáticas de progesterona, foi semelhante nos grupos que apresentaram morte embrionária e nos que mantiveram a gestação. Os níveis de estrogeno plasmático foram mais elevados no grupo das fêmeas que mantiveram a gestação. Os exames citológicos, microbiológicos e histopatológicos revelaram que a maioria das éguas com diagnóstico de morte embrionária eram portadoras de endometrites

Estudo da técnica de coleta, congelação e descongelação de embriões de caprinos (Capra hircus), da raça Saanen, portadores da translocação 5/15

This work studied the surgical technique for collecting embryos in the goat, using heterozygous donors for the 5/15 translocation, which were bred with translocated animals. At the same, time technical characteristics related to cryopreservation, thawing and cultivation of the structures were performed. Considering the superovulatory treatment, oestrus was observer 24 or 48 hours after removing the intravaginal sponge and 71 viable structures were yielded in 43 collections. The 1 -2 Propanediol proved to be an efficient cryoprotectant agent, and allowed the cultivation of all the embryos. This result was not observed when glycerol was used as a cryoprotectant agent. Adhesions due to multiple collections constitute a great challenge for the improvement of this technique, since they are considered a limiting factor to the use of donors in the beginning of their reproductive lives.No presente trabalho foi realizado um estudo da técnica cirúrgica de coleta de embriões na espécie caprina, utilizando-se doadoras heterozigotas para a translocação 5/15, acasaladas com animal translocado. Simultaneamente foram observados aspectos técnicos inerentes à criopreservação, descongelação e cultivo das estruturas recuperadas. Frente ao tratamento superovulatório, o cio foi observado em 24 ou 48 horas após a remoção da esponja intravaginal e foram recuperadas 71 estruturas viáveis em 43 coletas. O 1 -2 Propanodiol mostrou-se eficiente criopreservador, permitindo cultivo de todos os embriões, fato não observado quando utilizou-se o Glicerol. As aderências provocadas pelas repetidas coletas constituem-se no grande desafio no sentido de se aperfeiçoar a técnica, pois são consideradas um fator limitante ao uso de doadores no início da vida reprodutiva

Henry Kamen. The War of Succession in Spain, 1700-1715. London, Weidenfeld and Nicolson ,1969. In-8°, XII-436 pages, 18 tableaux et 3 graphiques dans le texte.

Ozanam Denise. Henry Kamen. The War of Succession in Spain, 1700-1715. London, Weidenfeld and Nicolson ,1969. In-8°, XII-436 pages, 18 tableaux et 3 graphiques dans le texte.. In: Bibliothèque de l'école des chartes. 1973, tome 131, livraison 1. pp. 304-312

René Mathieu De Vienne (1919-1989)

Ozanam Denise. René Mathieu De Vienne (1919-1989). In: Bibliothèque de l'école des chartes. 1990, tome 148, livraison 2. pp. 516-517

J. F. BOSHER. French Finances, 1770-1795. From Business to Bureaucracy. Cambridge, Cambridge University Press, 1970.

Ozanam Denise. J. F. BOSHER. French Finances, 1770-1795. From Business to Bureaucracy. Cambridge, Cambridge University Press, 1970.. In: Bibliothèque de l'école des chartes. 1971, tome 129, livraison 2. pp. 497-501

Henry Kamen. The War of Succession in Spain, 1700-1715. London, Weidenfeld and Nicolson ,1969. In-8°, XII-436 pages, 18 tableaux et 3 graphiques dans le texte.

Ozanam Denise. Henry Kamen. The War of Succession in Spain, 1700-1715. London, Weidenfeld and Nicolson ,1969. In-8°, XII-436 pages, 18 tableaux et 3 graphiques dans le texte.. In: Bibliothèque de l'école des chartes. 1973, tome 131, livraison 1. pp. 304-312

J. F. BOSHER. French Finances, 1770-1795. From Business to Bureaucracy. Cambridge, Cambridge University Press, 1970.

Ozanam Denise. J. F. BOSHER. French Finances, 1770-1795. From Business to Bureaucracy. Cambridge, Cambridge University Press, 1970.. In: Bibliothèque de l'école des chartes. 1971, tome 129, livraison 2. pp. 497-501

How to Perform and Interpret Testicular Fine Needle Aspiration in Stallions

Although several methods of testicular biopsy have been proposed previously, testicular fine needle aspiration (FNA) has proved to be the simplest, the most rapid, inexpensive, and overall the least invasive technique for obtaining testicular biopsies Testicular FNA is indicated for fertility investigations in stallions with oligozoospermia or azoospermia It is also used for differential diagnosis of testicular enlargement After sedation the stallion's testis is punctured to obtain testicular parenchyma samples containing cells mainly from the seminiferous epithelium the material obtained is used to perform smears which are analyzed for identification and quantification of term cells and Sertoli cells The results are based on the presence of the cell types found in the smears and the proportions of Sertoli cells per germ cells In addition to being a very useful diagnostic tool, testicular FNA is also used for follow-up examinations, as it is minimally invasiv

Reproductive characteristics of stallions during the breeding and non-breeding season in a tropical region

The objective of this study was to investigate reproductive characteristics of stallions at a tropical zone in the breeding and non-breeding seasons. The following parameters were assessed: testicular volume; semen quality; and serum concentrations of LH, FSH, and testosterone; in addition to the percentages of germ cells and proportions of germ cells/Sertoli cells by testicular cytology in stallions. Semen was collected from eight adult stallions twice a week during a 12-week period in both seasons (6 weeks before and 6 weeks after the summer and winter solstices). Jugular blood samples were collected periodically for hormone analysis by radioimmunoassay during the same periods. Testicular measures and cytological samples were taken at the end of each period. Mean concentration of testosterone was significantly higher (P = 0.04) during the breeding season and the proportion of Sertoli cells/100 germ cells in cytological smears was significantly lower during the breeding season (P = 0.0001). Effects of season were not significant either for testicular volume or for any semen parameter (P > 0.05). Seasonal changes in the mean concentrations of LH and FSH were not observed (P > 0.05). There were also no significant differences in the mean percentages of germ cell types between both seasons (P > 0.05). Lack of seasonal differences in the testicular volume and semen parameters of tropical stallions are probably due to the small variation in duration of natural light between the observed periods, slightly under 3 h.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

COMPARISON BETWEEN TWO METHODS OF STAINING FOR ASSESSMENT OF MORPHOLOGY AND ACROSOME IN DOMESTIC CAT (Felis catus) SPERMATOZOA COMPARAÇÃO ENTRE DOIS MÉTODOS DE COLORAÇÃO PARA ANÁLISE MORFOLÓGICA E ACROSSOMAL DE ESPERMATOZÓIDES DE GATO DOMÉSTICO (Felis catus).

<span><font size="3"><font face="Times New Roman"><span><p align="justify">The objective of this study was to evaluate the efficiency of the modified Karras staining technique (KA) to analyze domestic cat sperm morphology by comparing it with the Fast Green FCF/ Rose Bengal staining (FR), previously used for this species. Four adult cats were used, from which sperm samples were collected four times in alternate days for each tom using an artificial vagina (n=16 ejaculates). Both staining techniques were performed for each ejaculate. For the FR staining technique, the semen <em>in natura</em> was diluted in 2.9% sodium citrate and, afterwards, in the staining solution. After 70 seconds, smears were made onto slide and dried at 37ºC. For the KA staining technique, previously made and formol saline fixed slides were sequentially immersed in Rose Bengal solution, Tannin<font size="2" color="#ff0000"><font size="3" color="#ff0000"> </font></font><font size="3">solution, and Victoria Blue B solution, and dried at room temperature. For sperm evaluation, 200 sperm cells were assessed for each staining technique in all ejaculate samples using a bright field microscope at 1000X magnification. Statistical <span>analysis used the non-parametric Wilcoxon test, establishing significance at p<0.05. For the KA staining technique, higher percentage of distal cytoplasmic droplets and lower percentage of sperm head defects were obtained when compared to the FR staining technique. This way, both staining techniques were not totally efficient for the assessment of morphological defects found in the domestic cat <em>in natura </em>spermatozoa.</span></font></p><font size="3"><span><span><p align="justify">KEY WORDS: Acrosome, domestic cat, spermatozoa, sperm morphology, staining.</p></span></span></font></span></font></font></span><p> </p> <font size="3"><font face="Times New Roman"><span style="color: black"><span><p align="justify">O objetivo do estudo foi avaliar a eficiência do método de coloração Karras modificado (KA) para a análise da morfologia espermática no gato doméstico através da comparação com a coloração Fast Green FCF/Rosa Bengala (FR), previamente utilizada para esta espécie. Utilizaram-se quatro gatos adultos, colhendo-se quatro vezes amostras de sêmen em dias alternados para cada animal através de vagina artificial (n=16 ejaculados). Para cada ejaculado, realizaram-se duas colorações. Para a coloração FR, o sêmen <em>in natura</em> foi diluído em citrato de sódio 2,9% e, posteriormente, em solução de coloração. Após setenta segundos, procedeu-se a esfregaços em lâminas, as quais foram secas a 37ºC. Para a coloração KA, os esfregaços previamente confeccionados e fixados em formol salino foram imersos seqüencialmente nas soluções de Rosa Bengala, Tanino e Azul Vitória e secas em temperatura ambiente. Avaliaram-se duzentas células para cada tipo de coloração em todos os ejaculados, usando-se microscópio de luz em aumento de 1.000X. Efetuou-se análise estatística mediante o teste não-paramétrico de Wilcoxon, estabelecendo diferença significativa quando p<0,05. Para a coloração de KA, obtiveram-se maior porcentagem de gota citoplasmática distal e menor porcentagem de defeitos de cabeça quando comparada à coloração FR. Assim, nenhuma das colorações mostrou-se totalmente eficiente na identificação dos defeitos de morfologia encontrados na avaliação do sêmen <em>in natura</em> de gatos domésticos.</p><span><p align="justify">PALAVRAS-CHAVES: Acrossomo, coloração, espermatozóide, gato doméstico, morfologia espermática.</p></span></span></span></font></font><p> </p&gt