Seasonal variation of rotavirus infection and G genotypes among children admitted with diarrhea.

<p>The graph shows the total number of rotavirus infected children admitted due to diarrhea (cases) per month with G genotypes.</p

A and B. Phylogenetic trees of the rotavirus nucleotide sequence of the partial VP7 and VP4 genes.

<p>The phylogenetic tree: Phylogenetic trees based on the nucleotide sequence of the partial VP7 gene (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097562#pone-0097562-g001" target="_blank">figure 1A</a>) and VP4 gene (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097562#pone-0097562-g001" target="_blank">figure 1B</a>) of rotaviruses from Tanzania with known rotavirus reference strains from GenBank database and rotavirus vaccine strains i.e Rotateq and Rotarix. Reference strains, vaccine strains and strains from this study are indicated by accession numbers. The Genius software package was used to build the tree with the UPGMA method and bootstrapped with 1,000 repetitions; The Kimura-2 substitution model was used. The bar indicates nucleotide substitutions per site.</p

Sequencing results of rotavirus G and P genotypes circulating in children with diarrhea (cases) and children without diarrhea (controls) in Dar es Salaam, Tanzania (n = 211; 190 cases and 21 controls).

<p>Sequencing results of rotavirus G and P genotypes circulating in children with diarrhea (cases) and children without diarrhea (controls) in Dar es Salaam, Tanzania (n = 211; 190 cases and 21 controls).</p

Prevalence of rotavirus infection in different age groups.

<p>The graph shows the prevalence of rotavirus from ELISA results per age group in cases and controls.</p