Immunohistochemistry for VEGF protein expression in chorionic villus.

<p>VEGF protein was expressed in the chorionic villus located in the cytoplasm (Original magnification ×600. VEGF intensity by immunohistochemistry staining was scored as following: 1=weak staining; 2=mild staining and 3=dark staining. *Based on the score of VEGF intensity, cases were classified into VEGF low-expression (low) for those cases in which of VEGF intensity scores were ≤1 and VEGF high-expression (high) for those cases in which of VEGF intensity scores were ≥2. Scores were generated and analyzed by LTQ-Orbitrap mass spectrometry. We found that the chorionic villus in RSA women that subsequently miscarried showed high expression of VEGF (n =24) (A), whereas this tissue showed low expression of VEGF during early pregnancy in women with normal pregnancies (n =16) (B).</p

Levels of serum VEGF and sFlt-1 in RSA patients and normal pregnancy women.

<p>(<b>A</b>) The levels of sFlt-1 in the RSA group were significantly higher than in the control group (<i>p</i><0.0001). (<b>B</b>) The levels of VEGF in the RSA group were significantly higher than in the control group (<i>p</i><0.0001). (<b>C</b>) The ratio of sFlt-1/VEGF in serum.</p

Immunohistochemistry for sFlt-1 protein expression in chorionic villus.

<p>sFlt-1 protein was expressed in the chorionic villus located in the cytoplasm (Original magnification ×600. sFlt-1 intensity by immunohistochemistry staining was scored as following: 1=weak staining; 2=mild staining and 3=dark staining. *Based on the score of sFlt-1 intensity, cases were classified into sFlt-1 low-expression (low) for those cases in which of sFlt-1 intensity scores were ≤1 and sFlt-1 high-expression (high) for those cases in which of sFlt-1 intensity scores were ≥2. Scores were generated and analyzed by LTQ-Orbitrap mass spectrometry. We found that the chorionic villus of RSA women that subsequently miscarried showed a high expression of sFlt-1 (n =26) (A), whereas this tissue showed a low expression of sFlt-1 during early pregnancy in women with normal pregnancies (n =18) (B).</p

Levels of serum VEGF and sFlt-1 in pregnant and non-pregnant women.

<p>(<b>A</b>) The levels of sFlt-1 in women with normal pregnancies were significantly higher than in women that were not pregnant (<i>p</i><0.05). (<b>B</b>) The levels of VEGF in women with normal pregnancies were significantly higher than in women that were not pregnant (<i>p</i><0.001).</p

Additional file 5: of Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection

Figure S5. Immunofluorescence images showing the positive expression of Mφ lineage markers MHC-II in iPS-Mφ (A), THP-1-Mφ (B) and ES-Mφ (C). Nuclei are labeled with DAPI. Bar = 100 μm. (TIFF 1462 kb

Additional file 3: of Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection

Figure S3. Immunofluorescence images showing the positive expression of Mφ lineage markers CD40 in iPS-Mφ (A), THP-1-Mφ (B) and ES-Mφ (C). Nuclei are labeled with DAPI. Bar = 100 μm. (TIFF 1548 kb

Additional file 2: of Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection

Figure S2. Immunofluorescence images showing the positive expression of Mφ lineage markers CD14 in iPS-Mφ (A), THP-1-Mφ (B) and ES-Mφ (C). Nuclei are labeled with DAPI. Bar = 100 μm. (TIFF 1337 kb