Artemether-Lumefantrine treatment combined with albendazole and ivermectin induced genotoxicity and hepatotoxicity through oxidative stress in Wistar rats

AbstractMass drug administration against malaria and parasitic worm co-infections is capable of increasing health risk. This study investigated the hepatotoxicity, genotoxicity and oxidative stress induced by combinations of Arthemether-Lumefantrine (A-L) with Albendazole (ABZ) and Ivermectin (IVR) treatments in rats. 65 rats equally distributed into 13 groups were orally gavaged human therapeutic doses (×1.0), half of the doses (×0.5) and twice the doses (×2.0) of these drugs per body weights. Blood, liver and bone marrow cells were analyzed for serum biochemistry, histopathology and micronucleated polychromatic erythrocytes (MNPCE) respectively. Treated rats showed clinical signs of toxicity. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin and malondialdehyde (MDA) significantly increase with concomitant decrease in superoxide dismutase (SOD) and catalase (CAT) in the serum. Liver histology revealed single cell hepatocellular necrosis and kupffer cell hyperplasia, multiple foci vacuolar changes in the hepatocytes, thinning of hepatic cord and congestion of the sinusoids by inflammatory cells. Also, frequency of MNPCE significantly increased in the treated rats. The findings revealed that combine treatment of A-L with ABZ and IVR mostly at ×2.0 and ×1.0 induced liver dysfunctions and somatic mutations through oxidative stress in rats. These suggest health risk in wildlife and human populations during treatments with these drug combinations

Ethno - Botanical Survey Of Plant Species Used For Mosquito Control In Nigeria

In Nigeria, there is paucity of information on plants used to repel or kill mosquitoes despite the abundant plant species in the country’s tropical rain forests. Majority of available data are on the traditional use of plants for curative purposes. This study documents some plant species used for insecticidal or repellent purposes against mosquitoes in Nigeria. An ethno-botanical survey was carried out in four geo-political zones in the country using structured questionnaires and focus group discussion. A total of six plant species belonging to five families were reported in Delta state with Conyza Canadensis (Compositae) being the most utilized (50%) and Aspilia africana (Compositae), the least mentioned (5.60%). In Enugu state, a total of nine plant species belonging to eight families were mentioned by respondents with Aframomum melegueta the most utilised (26.09%) while Agava sisalana and Dracaena manii belonging to the families Agavaceae and Dracaenaceae were the least mentioned (1.09% each). A total of six plant species belonging to five families were reported in Kaduna State with Lantana camara belonging to the family Verbenaceae accounting for 58.82% of the utilised plant species for mosquito control while Ipomea asarifolia, Terminalia catappa and Citrullus vulgaris belonging to the families Convolvulaceae, Combretaceae and Cucurbitaceae respectively were the least utilized (5.88% each). Lagos State recorded the highest number of plant species (twenty five) belonging to eighteen families. Spondia mombin belonging to the family Lilaceae was the most mentioned (7.33%) while the least mentioned (0.37% each) were Magnifera indica, Baphia nitida and Pennisetum purpeum belongingto the families Anacardiaceae, Fabaceae and Poaceae respectively. There is a need for further investigation on these plant species as they may constitute potential sources of cidal and repellent compounds against a wide range of insect pests. Keywords: Ethno-botanical survey, insecticidal, repellence, mosquitoes, plant specie