The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties

Oxidative stress and inflammation play a key role in the age-related decline in the respiratory function. Adipokine in relation to the metabolic and inflammatory systems is attracting growing interest in the field of respiratory dysfunction. The present clinical and experimental studies investigated the role of the disulfide bond-forming oxidoreductase A-like protein (DsbA-L) gene, which has antioxidant and adiponectin multimeric (i.e. activation) properties, on the respiratory function of the elderly. We performed a retrospective longitudinal genotype-phenotype relationship analysis of 318 Japanese relatively elderly participants (mean age ± standard deviation: 67.0 ± 5.8 years) during a health screening program and an in vitro DsbA-L knock-down evaluation using 16HBE14o-cells, a commonly evaluated human airway epithelial cell line. The DsbA-L rs1917760 polymorphism was associated with a reduction in the ratio of forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) and %FEV1 and with the elevation of the prevalence of FEV1/FVC < 70%. We also confirmed that the polymorphism was associated with a decreased respiratory function in relation to a decrease in the ratio of high-molecular-weight adiponectin/total adiponectin (as a marker of adiponectin multimerization) and an increase in the oxidized human serum albumin (as an oxidative stress marker). Furthermore, we clarified that DsbA-L knock-down induced oxidative stress and up-regulated the mucus production in human airway epithelial cells. These findings suggest that the DsbA-L gene may play a role in protecting the respiratory function of the elderly, possibly via increased systemic adiponectin functions secreted from adipocytes or through systemic and/or local pulmonary antioxidant properties

ジドウヨウ ジョウドウ チノウ シャクド ノ カイハツ ト ソノ シンライセイ ダトウセイ ノ ケントウ

In this study, Emotional Intelligence Scale for adults（EQS）（Uchiyama, Shimai, Utsugi, and Otake, 2001）was reviced in consideration of the elementary school children’s life scene, and Emotional Intelligence Scale for children（EQS?C）that consisted of 38 items was made. EQS?C and Five Factor Personality Inventory for Children（FFP?C）（Soga, 1999）were carried out in the elementary school of 4th, 5th, and 6th grade, and effective answers were received from 178 children. The results were as follows. All the 12 factors of EQS?C were correlated positively with the cooperation factor and the control factor of FFP?C. On the other hand, the 3 factors in the 5 factors in the intrapersonal section of EQS?C and the 4 factors in the 5 factors in the situational section of EQS?C were correlated negaitively with emotional instability factor of FFP?C. The α coefficient of Cronbach in EQS?C showed the value that exceeded 0.8 greatly in all three sections（intrapersonal, interpersonal, and situational）. Next, the re?test of EQSC was carried out for the 105 children out of the 178 children of the above, and effective answers were received from 103 children. The results showed that the the reliability coefficient value exceeded 0.8 in all theree sections. Furthermore, EQS?C were carried out in the elementary school of the 6th grade 3 classes. The effective answers were received from 68 children, and the homeroom teacher of each class was asked with the rating by the teacher evaluation edition of EQS?C. The results showed that relations between the self?evaluation and the teacher’s evaluation by EQS?C was obtained from the viewpoint of statistics

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博士(政策科学)同志社大