2-[2-(1H-Imidazol-1-yl)-2-adamant­yl]phenol

In the title mol­ecule, C19H22N2O, the imidazole and benzene rings form a dihedral angle of 84.53 (5)°. In the crystal, classical inter­molecular O—H⋯N hydrogen bonds pair the mol­ecules into centrosymmetric dimers, and C—H⋯π inter­actions further link these dimers into columns propagated in [100]

Marker Loci in Brucella Genome for Differential PCR Indication of Pathogenic strains

Objective of this work was to develop the algorithms for differential PCR indication of Brucella genus strains using databases of their genomes. Materials and methods .Resources of the National Center for Biotechnology Information (NCBI) and BLAST and Vector NTI 9.1.0 software utilities. For PCR amplification, B. suis, B. abortus, B. melitensis nucleic acids, as well as plasmid DNA with marker insertions were used. Results and conclusions. We assessed brucella gene sequences, some of which are found in Brucella genus bacteria, others only in representatives of B. melitensis, and the third ones – only in representatives of B. abortus. As a result of primers and probes designing for indication of Brucella genus bacteria and representatives of B. melitensis and B. abortus species, criteria for marker sequence amplification have been established. These criteria provide for simultaneous differentiation in a single reaction. The determination of strain differences within one species of Brucella is described in multilocus VNTR assay technique, and the profiles of tandem repeats of various B. melitensis and B. abortus strains are available in the public domain. To monitor the progress of amplification, a positive control has been developed that has the nucleotide sequence of all marker regions. The text of the paper discloses all the nucleotide sequences of primers, probes and positive control, which makes it possible to independently acquire them in competent organizations

Сполиготипирование туберкулезных микобактерий, выделенных от человека и крупного рогатого скота

Objective: to analyze the species and genetic families of tuberculous mycobacteria isolated from humans and cattle by spoligotyping.Subjects and methods. Biological materials collected in the cattle, livestock farmers (who underwent annual fluorographic examinations) and tuberculosis patients were studied. To identify mycobacteria, the spoligotyping method was used.Results. The article assesses the variability of sites within direct repeats (spacers), which are used in laboratory diagnostics for spoligotyping of mycobacteria and identification of tuberculosis pathogens. The frequency of combinations of different spacers in the analyzed mycobacteria in the specimens identified as Mycobacterium tuberculosis complex is compared with the spoligotyping profile of known mycobacteria thus establishing their belonging to a specific genetic family The studied isolates were tested by microscopic, bacteriological, and molecular genetic (PCR) methods. Based on the results of spoligotyping, it was found out that they belonged to genetic families of M. tuberculosis of Beijing, LAM, and Haarlem.Цель исследования: анализ видов и генетических линий туберкулезных микобактерий, выделенных от человека и крупного рогатого скота, 2 методом сполиготипирования.Материалы и методы. Объектом исследования служил биологический материал от крупного рогатого скота, а также работников животноводческих ферм (ежегодно проходивших флюорографическое исследование) и больных туберкулезом пациентов. Для идентификации микобактерий использовали метод сполиготипирования.Результаты. Представлена оценка вариабельности участков внутри прямых повторов (спейсеров), которые в лабораторной диагностике используются при сполиготипировании микобактерий и идентификации возбудителей туберкулеза. Сопоставление встречаемости комбинаций различных спейсеров у анализируемых микобактерий в исследуемом материале, идентифицированных как Mycobacteriumtuberculosiscomplex, со сполигопрофилем известных микобактерий устанавливает принадлежность их к конкретной генетической линии. Исследованные изоляты изучены микроскопическими, бактериологическими и молекулярно-генетическими (ПЦР) методами. По результатам сполиготипирования определена их принадлежность к генетическим линиям M. tuberculosisBeijing, LAM и Haarlem

Modern Trends of Organic Chemistry in Russian Universities

© 2018, Pleiades Publishing, Ltd. This review is devoted to the scientific achievements of the departments of organic chemistry in higher schools of Russia within the past decade

Three-Component Condensation of β-Ketonitriles, 4-Fluorobenzaldehyde, and Secondary Cyclic Amines

A new three-component condensation of β-ketonitriles, 4-fluorobenzaldehyde, and secondary cyclic amines was developed. A possible reaction mechanism has been proposed including Knoevenagel condensation followed by aromatic nucleophilic substitution. It was found that in the case of 3-oxopropanenitrile bearing the 6-amino-1,3-dimethyluracil moiety, the reaction is not accompanied by fluorine substitution in the Knoevenagel adduct, and the Michael addition of a secondary amine occurs followed by oxidation

Three-Component Condensation of β-Ketonitriles, 4-Fluorobenzaldehyde, and Secondary Cyclic Amines

A new three-component condensation of β-ketonitriles, 4-fluorobenzaldehyde, and secondary cyclic amines was developed. A possible reaction mechanism has been proposed including Knoevenagel condensation followed by aromatic nucleophilic substitution. It was found that in the case of 3-oxopropanenitrile bearing the 6-amino-1,3-dimethyluracil moiety, the reaction is not accompanied by fluorine substitution in the Knoevenagel adduct, and the Michael addition of a secondary amine occurs followed by oxidation

Reactions of <i>o</i>‑Quinone Methides with Pyridinium Methylides: A Diastereoselective Synthesis of 1,2-Dihydronaphtho[2,1‑<i>b</i>]furans and 2,3-Dihydrobenzofurans

A simple, general route to the 1,2-dihydronaphtho­[2,1-<i>b</i>]­furans and 2,3-dihydrobenzofurans substituted at C-2 by an acyl or aryl group, starting from phenolic Mannich bases and pyridinium ylides, has been developed. The mechanism of the reaction is believed to involve the formation of the <i>o</i>-quinone methide intermediate, Michael-type addition of the ylide to the <i>o</i>-quinone methide, followed by intramolecular nucleophilic substitution

Reaction of Push–Pull Enaminoketones and <i>in Situ</i> Generated <i>ortho</i>-Quinone Methides: Synthesis of 3‑Acyl‑4<i>H</i>‑chromenes and 2‑Acyl‑1<i>H</i>‑benzo[<i>f</i>]chromenes as Precursors for Hydroxybenzylated Heterocycles

A simple and efficient method for the synthesis of 4<i>H</i>-chromenes and 1<i>H</i>-benzo­[<i>f</i>]­chromenes containing a trifluoroacetyl or aroyl group in the pyran ring from <i>o</i>-quinone methide precursors and push–pull enaminoketones has been developed. The chromenes are presumably formed through an initial oxa-Diels–Alder reaction, followed by an elimination of amine. The possibility of further transformations of given chromenes to <i>o</i>-hydroxybenzylated pyrazoles, isoxazoles, and pyridines has been demonstrated