Additional file 1: Table S1. of Analysis of heat shock protein 70 gene polymorphisms Mexican patients with idiopathic pulmonary fibrosis

Genotype frequencies of HSPA1L, HSPA1A and HSPA1B in different populations. (DOC 55 kb

Prevalent HLA Class II Alleles in Mexico City Appear to Confer Resistance to the Development of Amebic Liver Abscess

<div><p>Amebiasis is an endemic disease and a public health problem throughout Mexico, although the incidence rates of amebic liver abscess (ALA) vary among the geographic regions of the country. Notably, incidence rates are high in the northwestern states (especially Sonora with a rate of 12.57/100,000 inhabitants) compared with the central region (Mexico City with a rate of 0.69/100,000 inhabitants). These data may be related to host genetic factors that are partially responsible for resistance or susceptibility. Therefore, we studied the association of the <i>HLA-DRB1</i> and <i>HLA-DQB1</i> alleles with resistance or susceptibility to ALA in two Mexican populations, one each from Mexico City and Sonora. Ninety ALA patients were clinically diagnosed by serology and sonography. Genomic DNA was extracted from peripheral blood mononuclear cells. To establish the genetic identity of both populations, 15 short tandem repeats (STRs) were analyzed with multiplexed PCR, and the allelic frequencies of HLA were studied by PCR-SSO using LUMINEX technology. The allele frequencies obtained were compared to an ethnically matched healthy control group (146 individuals). We observed that both affected populations differed genetically from the control group. We also found interesting trends in the population from Mexico City. <i>HLA-DQB1*02</i> allele frequencies were higher in ALA patients compared to the control group (0.127 vs 0.047; <i>p</i>= 0.01; <i>pc</i>= NS; OR= 2.9, 95% CI= 1.09-8.3). The less frequent alleles in ALA patients were <i>HLA-DRB1*08</i> (0.118 vs 0.238 in controls; <i>p</i>= 0.01; <i>pc</i>= NS; OR= 0.42, 95% CI= 0.19-0.87) and <i>HLA-DQB1*04</i> (0.109 vs 0.214; <i>p</i>= 0.02; <i>pc</i>= NS; OR= 0.40, 95% CI= 0.20-0.94). The haplotype <i>HLA-DRB1*08/-DQB1*04</i> also demonstrated a protective trend against the development of this disease (0.081 vs. 0.178; <i>p</i>=0.02; <i>pc</i>=NS; OR= 0.40, 95% CI= 0.16-0.93). These trends suggest that the prevalent alleles in the population of Mexico City may be associated with protection against the development of ALA.</p></div

Geographical locations of Mexican population studied herein.

<p>Geographical locations of Mexican population studied herein.</p

Different allelic frequencies of STRs between Mexico City and Sonora Populations.

<p>Different allelic frequencies of STRs between Mexico City and Sonora Populations.</p