15 research outputs found
Expression levels of , , , , and genes in healthy individuals (control) and chronic myeloid leukemia patients
<p><b>Copyright information:</b></p><p>Taken from "Common molecular pathways involved in human CD133+/CD34+ progenitor cell expansion and cancer"</p><p>http://www.cancerci.com/content/7/1/11</p><p>Cancer Cell International 2007;7():11-11.</p><p>Published online 8 Jun 2007</p><p>PMCID:PMC1904434.</p><p></p> Total RNA was extracted from purified mononuclear cells from peripheral blood samples. Transcript levels were quantified by real-time PCR and results are given as normalized gene expression. Horizontal bars indicate median expression values. Statistical significance: < 0.001 for ; < 0.01 for , , and ; < 0.05 for and
Expansion of stem and progenitor cells from UCB cultivated in the presence or absence of estradiol
<p><b>Copyright information:</b></p><p>Taken from "Common molecular pathways involved in human CD133+/CD34+ progenitor cell expansion and cancer"</p><p>Cancer Cell International 2007;7():11-11.</p><p>Published online 8 Jun 2007</p><p>PMCID:PMC1904434.</p><p></p> (A) Fold increment in CD133+/CD34+, CD133-/CD34+, and CD133+/CD34- cell number after 7, 14, and 21 days in culture. (B) Flow cytometry analysis showing percentage of mononucleated cells expressing CD133 prior to and after culturing for 7 days. Positive and isotype controls are provided in supplemental figure S2. (C) Functional clonogenic assay based on the frequency of colony forming units (CFU). Data correspond to fold expansion of myeloid and endothelial progenitors at culture day 7. GF = basal medium supplemented with growth factors only (SCF, IL3, IL6, and Flt3-ligand); E2 = medium supplemented with growth factors plus 10 nM estradiol. Statistical significance for GF vs. E2 comparisons: * < 0.01, **< 0.001
Relative gene expression levels after 1h-treatment with 10 nM estradiol
<p><b>Copyright information:</b></p><p>Taken from "Common molecular pathways involved in human CD133+/CD34+ progenitor cell expansion and cancer"</p><p>Cancer Cell International 2007;7():11-11.</p><p>Published online 8 Jun 2007</p><p>PMCID:PMC1904434.</p><p></p> Transcript levels were quantified by real-time PCR in CD133+/CD34+ cells either with or without estradiol (control). Data are expressed as log2 of fold change. Negative values indicate repression. Statistical significance: < 0.001 for all comparisons
Global fitted (dotted line) SPR data of murine MX35 and Rebmab200 binding to immobilized synthetic NaPi2b epitope.
<p>MX35 (A) and Rebmab200 (B) were injected at concentrations ranging from 5 to 80 nM. After a 10 min association phase, the dissociation phase was followed for additional 10 min. Following double subtractive referencing, the curves were plotted using a 1â¶1 Langmuir binding model, using Biacore T100 Evaluation Software. The solid line represents the experimental data and the dotted line the mathematical model for the binding of MX35 and Rebmab200 to the synthetic NaPi2b epitope.</p
Immunoglobulin HC and LC mRNA levels and productivity of the most representative Rebmab200 clones as determined by RT-qPCR.
<p><b>GAPDH was used as a reference gene.</b> Vector represents PER.C6Âźcells transfected with mock vector; PER.C6Âź corresponds to the parental cell. Error bars represent the standard deviation of triplicates. Qp represents specific productivity levels.</p
Immunoreactivity of MX35 and Rebmab200 in live cells by flow cytometry.
<p>Immunoreactivity of MX35 and Rebmab200 in live cells by flow cytometry.</p
Comparison of ADCC activity between MX35 and Rebmab200 (stable pool) over a range of mAb concentrations.
<p>The % of cytotoxicity represents antibody-mediated cell lysis measured by release of <sup>51</sup>Cr from labeled ovarian cancer cells (OVCAR-3). Effector cells were obtained from donated human peripheral blood. Rebmab100 was used as a positive control, and Zenapax (Roche) was used as a negative control. The assay was repeated with MCF-7 cells, a NaPi2b negative and Le<sup>Y</sup> positive (Rebmab100 antigen) tumor cell line, showing no ADCC results (data not shown).</p
Immunoreactivity of MX35 and Rebmab200 in tumor tissues by immunohistochemistry.
<p>Immunoreactivity of MX35 and Rebmab200 in tumor tissues by immunohistochemistry.</p
Comparison of the immuno-affinity of MX35 (left) at 1/50 dilution and Rebmab200 (right) at 1/100 dilution in parallel reactions using the same buffers and amplification and development conditions.
<p>Serial sections of serous papillary ovary adenocarcinoma (A), clear cell renal carcinoma (B), large cell poorly differentiated carcinoma of the lung (C) and invasive ductal carcinoma of the breast (D). Original magnificationâ=â100Ă.</p
Binding Affinity, Specificity and Comparative Biodistribution of the Parental Murine Monoclonal Antibody MX35 (Anti-NaPi2b) and Its Humanized Version Rebmab200
<div><p>The aim of this preclinical study was to evaluate the characteristics of the monoclonal antibody Rebmab200, which is a humanized version of the ovarian-specific murine antibody MX35. This investigation contributes to the foundation for future clinical α-radioimmunotherapy of minimal residual ovarian cancer with <sup>211</sup>At-Rebmab200. Here, the biodistribution of <sup>211</sup>At-Rebmab200 was evaluated, as was the utility of <sup>99m</sup>Tc-Rebmab200 for bioimaging. Rebmab200 was directly compared with its murine counterpart MX35 in terms of its <i>in-vitro</i> capacity for binding the immobilized NaPi2B epitope and live cells; we also assessed its biodistribution in nude mice carrying subcutaneous OVCAR-3 tumors. Tumor antigen and cell binding were similar between Rebmab200 and murine MX35, as was biodistribution, including normal tissue uptake and <i>in-vivo</i> tumor binding. We also demonstrated that <sup>99m</sup>Tc-Rebmab200 can be used for single-photon emission computed tomography of subcutaneous ovarian carcinomas in tumor-bearing mice. Taken together, our data support the further development of Rebmab200 for radioimmunotherapy and diagnostics.</p></div