2,269 research outputs found
Ras-mediated phosphorylation of a conserved threonine residue enhances the transactivation activities of c-Ets1 and c-Ets2
The Ras oncogene products regulate the expression of genes in transformed cells, and members of the Ets family of transcription factors have been implicated in this process. To determine which Ets factors are the targets of Ras signaling pathways, the abilities of several Ets factors to activate Ras-responsive enhancer (RRE) reporters in the presence of oncogenic Ras were examined. In transient transfection assay, reporters containing RREs composed of Ets-AP-1 binding sites could be activated 30-fold in NIH 3T3 fibroblasts and 80-fold in the macrophage-like line RAW264 by the combination of Ets1 or Ets2 and Ras but not by several other Ets factors that were tested in the assay. Ets2 and Ras also superactivated an RRE composed of Ets-Ets binding sites, but the Ets-responsive promoter of the c-fms gene was not superactivated. Mutation of a threonine residue to alanine in the conserved amino-terminal regions of Ets1 and Ets2 (threonine 38 and threonine 72, respectively) abrogated the ability of each of these proteins to superactivate reporter gene expression. Phosphoamino acid analysis of radiolabeled Ets2 revealed that Ras induced normally absent threonine-specific phosphorylation of the protein. The Ras-dependent increase in threonine phosphorylation was not observed in Ets2 proteins that had the conserved threonine 72 residue mutated to alanine or serine. These data indicate that Ets1 and Ets2 are specific nuclear targets of Ras signaling events and that phosphorylation of a conserved threonine residue is a necessary molecular component of Ras-mediated activation of these transcription factors
Differentiation of the mononuclear phagocyte system during mouse embryogenesis:the role of transcription factor PU.1
Maximum Likelihood Estimation in Gaussian Chain Graph Models under the Alternative Markov Property
The AMP Markov property is a recently proposed alternative Markov property
for chain graphs. In the case of continuous variables with a joint multivariate
Gaussian distribution, it is the AMP rather than the earlier introduced LWF
Markov property that is coherent with data-generation by natural
block-recursive regressions. In this paper, we show that maximum likelihood
estimates in Gaussian AMP chain graph models can be obtained by combining
generalized least squares and iterative proportional fitting to an iterative
algorithm. In an appendix, we give useful convergence results for iterative
partial maximization algorithms that apply in particular to the described
algorithm.Comment: 15 pages, article will appear in Scandinavian Journal of Statistic
Signatures of the disk-jet coupling in the Broad-line Radio Quasar 4C+74.26
Here we explore the disk-jet connection in the broad-line radio quasar
4C+74.26, utilizing the results of the multiwavelength monitoring of the
source. The target is unique in that its radiative output at radio wavelengths
is dominated by a moderately-beamed nuclear jet, at optical frequencies by the
accretion disk, and in the hard X-ray range by the disk corona. Our analysis
reveals a correlation (local and global significance of 96\% and 98\%,
respectively) between the optical and radio bands, with the disk lagging behind
the jet by days. We discuss the possible explanation for this,
speculating that the observed disk and the jet flux changes are generated by
magnetic fluctuations originating within the innermost parts of a truncated
disk, and that the lag is related to a delayed radiative response of the disk
when compared with the propagation timescale of magnetic perturbations along
relativistic outflow. This scenario is supported by the re-analysis of the
NuSTAR data, modelled in terms of a relativistic reflection from the disk
illuminated by the coronal emission, which returns the inner disk radius
. We discuss the global energetics in
the system, arguing that while the accretion proceeds at the Eddington rate,
with the accretion-related bolometric luminosity erg s , the jet total kinetic energy
erg s, inferred from the dynamical
modelling of the giant radio lobes in the source, constitutes only a small
fraction of the available accretion power.Comment: 9 pages and 6 figures, ApJ accepte
High Energy gamma-rays From FR I Jets
Thanks to Hubble and Chandra telescopes, some of the large scale jets in
extragalactic radio sources are now being observed at optical and X-ray
frequencies. For the FR I objects the synchrotron nature of this emission is
surely established, although a lot of uncertainties - connected for example
with the particle acceleration processes involved - remain. In this paper we
study production of high energy gamma-rays in FR I kiloparsec-scale jets by
inverse-Compton emission of the synchrotron-emitting electrons. We consider
different origin of seed photons contributing to the inverse-Compton
scattering, including nuclear jet radiation as well as ambient, stellar and
circumstellar emission of the host galaxies. We discuss how future detections
or non-detections of the evaluated gamma-ray fluxes can provide constraints on
the unknown large scale jet parameters, i.e. the magnetic field intensity and
the jet Doppler factor. For the nearby sources Centaurus A and M 87, we find
measurable fluxes of TeV photons resulting from synchrotron self-Compton
process and from comptonisation of the galactic photon fields, respectively. In
the case of Centaurus A, we also find a relatively strong emission component
due to comptonisation of the nuclear blazar photons, which could be easily
observed by GLAST at energy ~10 GeV, providing important test for the
unification of FR I sources with BL Lac objects.Comment: 39 pages, 6 figures included. Modified version, accepted for
publication in Astrophysical Journa
Rapid X-ray Declines and Plateaus in Swift GRB Light Curves Explained by A Highly Radiative Blast Wave
GRB X-ray light curves display rapid declines followed by a gradual
steepening or plateau phase in >~ 30% of GRBs in the Swift sample. Treating the
standard relativistic blastwave model in a uniform circumburst medium, it is
shown that if GRBs accelerate ultra-high energy cosmic rays through a Fermi
mechanism, then the hadronic component can be rapidly depleted by means of
photopion processes on time scales ~100 - 10^4 s after the GRB explosion. While
discharging the hadronic energy in the form of ultra-high energy cosmic ray
neutrals and escaping cosmic-ray ions, the blast wave goes through a strongly
radiative phase, causing the steep declines observed with Swift. Following the
discharge, the blast wave recovers its adiabatic behavior, forming the observed
plateaus or slow declines. These effects are illustrated by calculations of
model bolometric light curves. The results show that steep X-ray declines and
plateau features occur when GRB sources take place in rather dense media, with
n >~ 100 cc out to >~ 10^17 cm.Comment: 13 pages, 7 figs, minor changes from previous version; accepted for
publication in Ap
Radiation from the Relativistic Jet: a Role of the Shear Boundary Layer
Recent radio and optical large scale jets' observations suggest a
two-component jet morphology, consisting of a fast central spine surrounded
with a boundary layer with a velocity shear. We study radiation of electrons
accelerated at such boundary layers as an option for standard approaches
involving internal shocks in jets. The acceleration process in the boundary
layer yields in a natural way a two component electron distribution: a
power-law continuum with a bump at the energy, where energy gains equal
radiation losses, followed by a cut-off. For such distributions we derive the
observed spectra of synchrotron and inverse-Compton radiation, including
comptonization of synchrotron and CMB photons. Under simple assumptions of
energy equipartition between the relativistic particles and the magnetic field,
the relativistic jet velocity at large scales and a turbulent character of the
shear layer, the considered radiation can substantially contribute to the jet
radiative output. In the considered conditions the synchrotron emission is
characterized by a spectral index of the radio-to-optical continuum being
approximately constant along the jet. A characteristic feature of the obtained
broad-band synchrotron spectrum is an excess at X-ray frequencies, similar to
the one observed in some objects by Chandra. As compared to the uniform jet
models, the velocity shear across the radiating boundary region leads to
decrease and frequency dependence of the observed jet-counterjet radio
brightness asymmetry. We conclude that a careful investigation of the
observational data looking for the derived effects can allow to evaluate the
role of the boundary layer acceleration processes and/or impose constraints for
the physical parameters of such layers in large scale jets.Comment: 30 pages, 4 figures included. Modified version, accepted for
publication in Astrophysical Journa
Intracerebroventricular Ghrelin Administration Increases Depressive-Like Behavior in Male Juvenile Rats
Major depressive disorder (MDD) is arguably the largest contributor to the global disease and disability burden, but very few treatment options exist for juvenile MDD patients. Ghrelin is the principal hunger-stimulating peptide, and it has also been shown to reduce depressive-like symptoms in adult rodents. We examined the effects of intracerebroventricular (icv) injection of ghrelin on depressive-like behavior. Moreover, we determined whether ghrelin increased neurogenesis in the hippocampus. Ghrelin (0.2-nM, 0.5-nM, and 1.0-nM) was administered acutely by icv injection to juvenile rats to determine the most effective dose (0.5-nM) by a validated feeding behavior test and using the forced swim test (FST) as an indicator of depressive-like behavior. 0.5-nM ghrelin was then administered icv against an artificial cerebrospinal fluid (aCSF) vehicle control to determine behavioral changes in the tail suspension test (TST) as an indicator of depressive-like behavior. Neurogenesis was investigated using a mitogenic paradigm, as well as a neurogenic paradigm to assess whether ghrelin altered neurogenesis. Newborn hippocampal cells were marked using 5′-bromo-2′-deoxyuridine (BrdU) administered intraperitoneally (ip) at either the end or the beginning of the experiment for the mitogenic and neurogenic paradigms, respectively. We found that ghrelin administration increased immobility time in the TST. Treatment with ghrelin did not change mitogenesis or neurogenesis. These results suggest that ghrelin administration does not have an antidepressant effect in juvenile rats. In contrast to adult rodents, ghrelin increases depressive-like behavior in male juvenile rats. These results highlight the need to better delineate differences in the neuropharmacology of depressive-like behavior between juvenile and adult rodents
Multifrequency Photo-polarimetric WEBT Observation Campaign on the Blazar S5 0716+714: Source Microvariability and Search for Characteristic Timescales
Here we report on the results of the WEBT photo-polarimetric campaign
targeting the blazar S5~0716+71, organized in March 2014 to monitor the source
simultaneously in BVRI and near IR filters. The campaign resulted in an
unprecedented dataset spanning \,h of nearly continuous, multi-band
observations, including two sets of densely sampled polarimetric data mainly in
R filter. During the campaign, the source displayed pronounced variability with
peak-to-peak variations of about and "bluer-when-brighter" spectral
evolution, consisting of a day-timescale modulation with superimposed hourlong
microflares characterized by \,mag flux changes. We performed an
in-depth search for quasi-periodicities in the source light curve; hints for
the presence of oscillations on timescales of \,h and \,h do
not represent highly significant departures from a pure red-noise power
spectrum. We observed that, at a certain configuration of the optical
polarization angle relative to the positional angle of the innermost radio jet
in the source, changes in the polarization degree led the total flux
variability by about 2\,h; meanwhile, when the relative configuration of the
polarization and jet angles altered, no such lag could be noted. The
microflaring events, when analyzed as separate pulse emission components, were
found to be characterized by a very high polarization degree () and
polarization angles which differed substantially from the polarization angle of
the underlying background component, or from the radio jet positional angle. We
discuss the results in the general context of blazar emission and energy
dissipation models.Comment: 16 pages, 17 Figures; ApJ accepte
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