32 research outputs found

    PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli

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    We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2

    Antibiotics residues in food - still actual problem

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