Fundamental Study on Electrical Discharge Machining

The generation mechanism of crater in electrical discharge machining is analyzed with a single pulse discharge device for alloy tool steel, black alumina ceramics, cermet and cemented carbide, investigating the gap voltage, the discharge current, the shape of crater, the wear of electrode and so on. The experimental analysis makes it clear that the shape of crater has a characteristic feature for the kind of workpiece. The shape of electrode, which changes with the wear by an electric spark, has a significant effect on the shape of crater. The diameter and the depth of crater have a close relation to the discharge energy for alloy tool steel, black alumina ceramics and cermet, while those for cemented carbide are related to the discharge current. The shape factor which is the ratio of the depth to the diameter of crater is different for the work material

3-D Kinematics of Water Masers in the W51A Region

We report proper motion measurements of water masers in the massive-star forming region W51A and the analyses of the 3-D kinematics of the masers in three maser clusters of W51A (W51 North, Main, and South). In W~51 North, we found a clear expanding flow that has an expansion velocity of ~70 km/s and indicates deceleration. The originating point of the flow coincides within 0.1 as with a silicon-monoxide maser source near the HII region W~51d. In W51 Main, no systematic motion was found in the whole velocity range (158 km/s =< V(lsr) =< -58 km/s) although a stream motion was reported previously in a limited range of the Doppler velocity (54 km/s =< V(lsr) =< 68 kms). Multiple driving sources of outflows are thought to explain the kinematics of W51 Main. In W51 South, an expansion motion like a bipolar flow was marginally visible. Analyses based on diagonalization of the variance-covariance matrix of maser velocity vectors demonstrate that the maser kinematics in W51 North and Main are significantly tri-axially asymmetric. We estimated a distance to W51 North to be 6.1 +/- 1.3 kpc on the basis of the model fitting method adopting a radially expanding flow.Comment: 20 pages, 8 figures, 8 tables, appear in the NRO report No. 564 (ftp://ftp.nro.nao.ac.jp/nroreport/PASJ-W51.pdf) and will appear in Publ. Astron. Soc. Japan, Vol. 54, No. 5 (10/25 issue

VLBI Monitoring Observations of Water Masers Around the Semi-Regular Variable Star R Crateris

We monitored water-vapor masers around the semi-regular variable star R Crateris with the Japanese VLBI Network (J-Net) at the 22 GHz band during four epochs with intervals of one month. The relative proper motions and Doppler-velocity drifts of twelve maser features were measured. Most of them existed for longer than 80 days. The 3-D kinematics of the features indicates a bipolar expanding flow. The major axis of the asymmetric flow was estimated to be at P.A. = 136 degrees. The existence of a bipolar outflow suggests that a Mira variable star had already formed a bipolar outflow. The water masers are in a region of apparent minimum radii of 1.3 x 10^12 m and maximum radii of 2.6 x 10^12 m, between which the expansion velocity ranges from 4.3 to 7.4 km/s. These values suggest that the water masers are radially accelerated, but still gravitationally bound, in the water-maser region. The most positive and negative velocity-drifting features were found relatively close to the systemic velocity of the star. We found that the blue-shifted features are apparently accelerated and the red-shifted apparently decelerated. The acceleration of only the blue-shifted features seems to be consistent with that of the expanding flow from the star.Comment: 15 pages, 5 figures, Accepted for publication in PASJ (2001), preprint can be obtained via WWW on http://www.nro.nao.ac.jp/library/report/list.htm

Distributions of biogeochemical parameters in the pool and interstitial waters in sand bar system of the Kizu River

Distributions of biogeochemical parameters in temporary pools (TAMARI), riparian side arm of river (WANDO) and interstitial waters were investigated in the sand bars along the lower reaches of the Kizu River. Dissloved inorganic nitrogen (DIN) and phosphate (DIP) concentrations in TAMARI and WANDO waters varied greatly compared with those in river waters. Low concentrations of DIN and DIP were often observed simultaneously in TAMARI waters. The DIN concentrations of most intersitial waters were similar or high in comparison to those of river waters. Low concentrations of DIN and DIP were often observed separately in intersitial waters. Concentrations of biogeochemical constituents clearly varied greatly in the waters of sand bar systems, and the relationships between each biogeochemical constituents were different between surface water (TAMARI and WANDO) and subsurface (intersitial) waters of the Kizu River.Article信州大学山地水環境教育研究センター研究報告 2: 63-67(2004)departmental bulletin pape

The Management of Constipation: Current Status and Future Prospects

Chronic constipation, a common condition, can have remarkably negative effects on a patient’s quality of life. Recent research has identified factors that may influence the prognosis of chronic constipation and suggests the need for adequate therapy. However, the major obstacles in this field were: (1) a small number of therapeutic options, (2) no clear diagnostic criteria, and (3) no effective method to collect information form the patients. These were due to the fact that bowel movement patterns vary widely among individuals, and also the functional constipation, including irritable bowel syndrome, is difficult to be distinguished from the chronic constipation. Recently, it has been demonstrated that the Rome IV diagnostic criteria of functional constipation and the Bristol stool form scale are useful for the objective evaluation and recording of stool. Based on these developments, and the increase of newly developed medicines the therapy for the constipation is significantly changing and therefore, if conventional therapy for chronic constipation is ineffective, switching of medicines is possible. Therefore, clinicians should update the information of these newly developed drugs available in clinics and diagnostic criteria. For this purpose, in this chapter, we have summarized the perspective on the current paradigm of treatment for chronic constipation focusing on recently introduced therapeutic drugs

Heme-mediated inhibition of Bach1 regulates the liver specificity and transience of the Nrf2-dependent induction of zebrafish heme oxygenase 1

The induction of the gene encoding heme oxygenase 1 (Hmox1, HO-1) by Nrf2 is unique compared with other Nrf2 targets. We previously showed that the Nrf2a-mediated induction of zebrafish hmox1a was liver specific and transient. We screened transcription factors that could repress the induction of hmox1a but not other Nrf2a targets and concluded that Bach1b was a prime candidate. In bach1b-knocked-down larvae, the induction of hmox1a was observed ectopically in nonliver tissues and persisted longer than normal fish, suggesting that Bach1 is the only regulator for both the liver-specific and transient induction of hmox1a. Co-knockdown of bach1b with its co-ortholog bach1a enhanced these effects. To determine why Bach1 could not repress the hmox1a induction in the liver, we analyzed the effects of a heme biosynthesis inhibitor, succinylacetone, and a heme precursor, hemin. Succinylacetone decreased the Nrf2a-mediated hmox1a induction, whereas pre-treatment with hemin caused ectopic induction of hmox1a in nonliver tissues, implying that the high heme levels in the liver may release the repressive activity of Bach1. Our results suggested that Bach1 regulates the liver specificity and transience of the Nrf2a-dependent induction of hmox1a and that heme mediates this regulation through Bach1 inhibition based on its level in each tissue

Cell–matrix interaction via CD44 is independently regulated by different metalloproteinases activated in response to extracellular Ca2+ influx and PKC activation

CD44 is an adhesion molecule that interacts with hyaluronic acid (HA) and undergoes sequential proteolytic cleavages in its ectodomain and intramembranous domain. The ectodomain cleavage is triggered by extracellular Ca2+ influx or the activation of protein kinase C. Here we show that CD44-mediated cell–matrix adhesion is terminated by two independent ADAM family metalloproteinases, ADAM10 and ADAM17, differentially regulated in response to those stimuli. Ca2+ influx activates ADAM10 by regulating the association between calmodulin and ADAM10, leading to CD44 ectodomain cleavage. Depletion of ADAM10 strongly inhibits the Ca2+ influx-induced cell detachment from matrix. On the other hand, phorbol ester stimulation activates ADAM17 through the activation of PKC and small GTPase Rac, inducing proteolysis of CD44. Furthermore, depletion of ADAM10 or ADAM17 markedly suppressed CD44-dependent cancer cell migration on HA, but not on fibronectin. The spatio-temporal regulation of two independent signaling pathways for CD44 cleavage plays a crucial role in cell–matrix interaction and cell migration