Blood utilization in patients with burn injury and association with clinical outcomes (CME)

Uncontrolled bleeding is an important cause of increased transfusion in burn victims; however, description of blood utilization patterns in the burn population is lacking

DNA and Inflammatory Mediators in Bronchoalveolar Lavage Fluid From Children With Acute Inhalational Injuries:

Assess the feasibility of using serial bronchoalveolar lavage fluids (BALF) to characterize the course of cell damage and inflammation in airways of pediatric patients with acute burn or inhalation injury

Visual findings in children exposed to Zika in utero in Nicaragua

Knowledge regarding the frequency of ocular abnormalities and abnormal visual function in children exposed to Zika virus (ZIKV) in utero but born without congenital Zika syndrome (CZS) is limited. We hypothesized that children exposed to ZIKV in utero born without CZS may have visual impairments in early childhood. We performed ophthalmic examination between 16 and 21 months of age and neurodevelopment assessment at 24 months of age with the Mullen Scales of Early Learning test (MSEL) on children enrolled in a cohort born to women pregnant during and shortly after the ZIKV epidemic in Nicaragua (2016–2017). ZIKV exposure status was defined based on maternal and infant serological testing. Visual impairment was defined as abnormal if the child had an abnormal ophthalmic exam and/or low visual reception score in the MSEL assessment. Of 124 children included in the analysis, 24 (19.4%) were classified as ZIKV-exposed and 100 (80.6%) unexposed according to maternal or cord blood serology. Ophthalmic examination showed that visual acuity did not differ significantly between groups, thus, 17.4% of ZIKV-exposed and 5.2% of unexposed had abnormal visual function (p = 0.07) and 12.5% of the ZIKV-exposed and 2% of the unexposed had abnormal contrast testing (p = 0.05). Low MSEL visual reception score was 3.2-fold higher in ZIKV-exposed than unexposed children, but not statistically significant (OR 3.2, CI: 0.8–14.0; p = 0.10). Visual impairment (a composite measure of visual function or low MESL visual reception score) was present in more ZIKV-exposed than in unexposed children (OR 3.7, CI: 1.2, 11.0; p = 0.02). However, the limited sample size warrants future investigations to fully assess the impact of in utero ZIKV exposure on ocular structures and visual function in early childhood, even in apparently healthy children

Visual findings in children exposed to Zika in utero in Nicaragua.

Knowledge regarding the frequency of ocular abnormalities and abnormal visual function in children exposed to Zika virus (ZIKV) in utero but born without congenital Zika syndrome (CZS) is limited. We hypothesized that children exposed to ZIKV in utero born without CZS may have visual impairments in early childhood. We performed ophthalmic examination between 16 and 21 months of age and neurodevelopment assessment at 24 months of age with the Mullen Scales of Early Learning test (MSEL) on children enrolled in a cohort born to women pregnant during and shortly after the ZIKV epidemic in Nicaragua (2016-2017). ZIKV exposure status was defined based on maternal and infant serological testing. Visual impairment was defined as abnormal if the child had an abnormal ophthalmic exam and/or low visual reception score in the MSEL assessment. Of 124 children included in the analysis, 24 (19.4%) were classified as ZIKV-exposed and 100 (80.6%) unexposed according to maternal or cord blood serology. Ophthalmic examination showed that visual acuity did not differ significantly between groups, thus, 17.4% of ZIKV-exposed and 5.2% of unexposed had abnormal visual function (p = 0.07) and 12.5% of the ZIKV-exposed and 2% of the unexposed had abnormal contrast testing (p = 0.05). Low MSEL visual reception score was 3.2-fold higher in ZIKV-exposed than unexposed children, but not statistically significant (OR 3.2, CI: 0.8-14.0; p = 0.10). Visual impairment (a composite measure of visual function or low MESL visual reception score) was present in more ZIKV-exposed than in unexposed children (OR 3.7, CI: 1.2, 11.0; p = 0.02). However, the limited sample size warrants future investigations to fully assess the impact of in utero ZIKV exposure on ocular structures and visual function in early childhood, even in apparently healthy children

DNA and Inflammatory Mediators in Bronchoalveolar Lavage Fluid From Children With Acute Inhalational Injuries

OBJECTIVE: Assess the feasibility of using serial bronchoalveolar lavage fluids (BALF) to characterize the course of cell damage and inflammation in airways of pediatric patients with acute burn or inhalation injury. DESIGN: Prospective, longitudinal descriptive pilot study. SETTING: Burn and Pediatric Intensive Care Units in a tertiary-care medical center. SUBJECTS: Six consecutive intubated, mechanically ventilated pediatric patients with acute inhalational injuries were studied. INTERVENTIONS: Serial BALF specimens from clinically-indicated bronchoscopies were used to measure DNA and cytokine levels. MEASUREMENTS AND MAIN RESULTS: BALF DNA levels for the 6 pediatric burn subjects were highest within the first 72 hours after burn injury and declined thereafter. At the early stages after injury, BALF DNA levels (median [min, max] 3789 [1170,11917] ng/ml) were similar to those in adult burn patients and pediatric cystic fibrosis or bronchiectasis patients, and higher than those in pediatric recurrent pneumonia patients. BALF DNA levels in children and adults with inhalation injury correlated significantly with BALF IL-6, IL-8, and TGF-β1 levels. The patient with the most severe early visible airway mucosal damage and soot pattern at bronchoscopy, as well as the most extensive burns, also had the highest average early BALF DNA level (11917ng/ml) and the longest ventilator course and hospital stay. Procedures were well tolerated. CONCLUSIONS: In children with acute burn and inhalational injury, airway cellular damage and inflammation (reflected in high BALF DNA levels) appear to peak during the first 72 hours after burns or inhalation injury followed by a slow decline. Serial analysis of factors in airway secretions is feasible and has the potential to reveal important pathophyisiologic pathways and therapeutic targets for treatment of acute inhalational injuries

Demographic and clinical characteristics of study population.

*<p>Within 1^st 14 days after injury.</p>**<p>Read by radiologist as having either infiltrates, edema, or atelectasis.</p

Comparison of IL-10 to IL-12p70 ratios in mainstem bronchial washings, between patients with the lowest PaO₂/FiO₂ ratios during the first 2 weeks post injury (PaO₂/FiO₂) of <200 (N = 16), and patients with PaO₂/FiO₂ ≥200 (N = 24).

<p>Bars represent mean ± SE. * P<0.05, t-test with Welch's correction for unequal variances.</p

Comparison of airway injury score (graded for soot, inflammation, epithelial sloughing and secretions at <72 hr post injury), between patients with the lowest PaO₂/FiO₂ ratios during the first 2 weeks post injury (PaO₂/FiO₂) of <200 (N = 16), and patients with PaO₂/FiO₂ ≥200 (N = 24).

<p>Bars represent mean ± SE. P = 0.11, t-test with Welch's correction for unequal variances.</p

Comparison of the lowest PaO₂/FiO₂ ratios during the first 2 weeks post injury (PaO₂/FiO₂) among patients with no bacterial pathogens isolated from respiratory secretions during the first 2 weeks (NEG), patients with bacterial pathogens of any type (POS), and patients with gram negative bacteria (GN POS).

<p>Patients in whom no bacterial cultures were collected are not included in this analysis. * = P<.05 vs. NEG.</p

Comparison of averaged early (<72 hr post injury) IL-10 concentrations in mainstem bronchial washings, between the lowest PaO₂/FiO₂ ratios ratios during the first 2 weeks post injury (PaO₂/FiO₂) of <200 (N = 16), and patients with PaO₂/FiO₂ ≥200 (N = 24).

<p>Bars represent mean ± SE. * P<0.05, t-test with Welch's correction for unequal variances.</p