Identification of superior doubled haploid maize (Zea mays) inbred lines derived from high oil content subtropical populations

High oil maize (HOM) hybrids, adapted to subtropical regions are needed to meet existing requirements forindustrial and livestock purposes. Recently, the doubled haploid (DH) breeding technology is applied to reduceconsiderably the time to generate homozygous maize lines, however, information of heterotic response, per seproductivity, stress, disease reaction, and adaptation to different environments are not known. In this work, fieldevaluations of per se and testcross high oil content DH lines adapted for subtropical environments were studiedto identify superior DH lines with good heterotic response and agronomic characteristics. White and yellow kerneltest crosses were formed using the different heterotic population as testers from which DH lines were derived.Test crosses were evaluated at three locations in Mexico. Superior DH lines from each tester presented highGCA effects through test crosses, with a range of grain yield across locations of 12.8 to 10.4 Mg ha-1 and 11.1to 9.1 Mg ha-1 respectively. Per se DH lines were observed and screened for adaptation, productivity, diseasereaction and other agronomic traits in five locations/year environments. Per se DH lines screening process acrossenvironments was able to obtain information for adaptation, productivity, and healthiness. Also of DH lines withhigh response of GCA, information regarding heterotic groups, and DH lines oil content determination was usedto identify outstanding DH lines for developing HOM high-yielding hybrids adapted for subtropical regions, withfewer costs and time. This research demonstrated that it was feasible to identify subtropical elite DH lines withhigh oil content