Controlling the root-knot nematode, Meloidogyne incognita in cucumber plants using some soil bioagents and some amendments under simulated field conditions

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Characterization of greater middle eastern genetic variation for enhanced disease gene discovery

The Greater Middle East (GME) has been a central hub of human migration and population admixture. The tradition of consanguinity, variably practiced in the Persian Gulf region, North Africa, and Central Asia1-3, has resulted in an elevated burden of recessive disease4. Here we generated a whole-exome GME variome from 1,111 unrelated subjects. We detected substantial diversity and admixture in continental and subregional populations, corresponding to several ancient founder populations with little evidence of bottlenecks. Measured consanguinity rates were an order of magnitude above those in other sampled populations, and the GME population exhibited an increased burden of runs of homozygosity (ROHs) but showed no evidence for reduced burden of deleterious variation due to classically theorized ‘genetic purging’. Applying this database to unsolved recessive conditions in the GME population reduced the number of potential disease-causing variants by four- to sevenfold. These results show variegated genetic architecture in GME populations and support future human genetic discoveries in Mendelian and population genetics

Essential oils from some Egyptian aromatic plants as an antimicrobial agent and for prevention of potato virus Y transmission by aphids

AbstractEssential oils from different Egyptian aromatic plants (Mentha piperita, Ocimum basilicum, and Thymus vulgaris) were tested for their inhibitory effect on some selected harmful bacteria and yeast (Escerichia coli, Psedumonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans). Aerial parts from plants were directed to steam distillation for essential oil extraction. Oil yields were expressed in relation to dry weight of plant material, which found to be 0.39%, 0.20% and 0.55% w/w for M. piperita, O. basilicum and T. vulgaris, respectively. The types and percentage of essential oil constituents were determined using gas chromatography (GC). GC data revealed that the main compounds from M. piperita were menthol (35.44%) and menthone (20.11%), O. basilicum main component was linalool (45.11%), while T. vulgaris oil main component was thymol (75.76%). Preparations studied for their inhibitory effect were raw oils for sensitivity test and in the form of emulsions for spraying application. Emulsions were prepared depending on commercial liquid dish wash soap (Peril®) and Tween 80 as emulsifying agents. Results of sensitivity tests indicated that the most effective oil against bacteria and yeast was that of O. basilicum followed by that of T. vulgaris. O. basilicum oil was highly effective on S. pyogenes giving a zone of 19mm more than that produced by Ampicillin, which was of 15mm in diameter. Oil of O. basilicum was slightly more effective on C. albicans when compared with clotrimazole as an antifungal agent. Spraying potato tubers with O. basilicum oil emulsified with soap prior to brown rot bacteria (Ralstonia solanacearum) infection and preservation at 4°C gave the best results followed by T. vulgaris oil with soap, as only 2 and 3 tubers out of 10 used shows rot symptoms, for O. basilicum and T. vulgaris oils, respectively. After feeding Myzus persicae aphids on potato virus y potyvirus (common strain) (PVYO) infected tobacco plants for 1h, insects were print-captured individually and virus was successfully detected by immunocapture reverse transcriptase polymerase chain reaction PC/IC-RT-PCR, as the 801bp coat protein gene (cp) bands were detected within agarose gel. Spraying tobacco plants with O. basilicum or T. vulgaris oils both emulsified with soap gave excellent results, as 8 and 6 plants out of 10 treated confirmed to be PVYO-free by giving negative I-ELISA results, respectively. It was also observed that adding soap as an emulsifier has a killing effect on aphids