Immunosenescence and vaccination of the elderly I. Age-related immune impairment

The sharp increase of life expectancy and the increasing ratio of ageing population pose new challenges for the public health system. The elderly suffer from more frequent and severe infections than young people. Theoretically, vaccination could protect the elderly against several infectious diseases, but due to their age-related immune impairment, vaccination might fail in many cases. Instead of ineffective vaccination campaigns, exploration and restoration of age-dependent dysregulation of their immune functions have to be placed into the focus of recent research. Frequent comorbidities in these people augment immune defects. Immunosenescence affects both the innate and adaptive immunity. Disturbances in macrophage-derived cytokine release and reduction of the natural killer cell mediated cytotoxicity lead to increased frequency of respiratory, gastrointestinal and skin infections. Although the humoral immunity retains most of its original activity through life span, ageing dampens the ability of B cells to produce antibodies against novel antigens. Age-related declination of the cellular immunity is the consequence of thymic atrophy, reduced output of new T lymphocytes, accumulation of anergic memory cells, deficiencies in the cytokine production and uncertain antigen presentation. Persistent infection by different herpesviruses and other parasites contribute to the loss of immunosurveillance and premature exhaustion of T cells

Different sensitivity to acid reaction of the AIDS virus and virus-producing cells: clinical conclusions

Sensitivity of the cell-free human immundeficiency virus type 1 (HIV-1) and its producer cells was Studied in acidic media between pH 7.4 and 4.9 vitro. The cytopathic effect, reverse transcriptase activity and p24 antigen production by survived viruses were monitored in indicator cell cultures. It was established that, the cell-free HIV-1 particles are very sensitive to acidity. Between pH 7.4 and 6.0 they loose infectivity gradually, but this process is irreversible under pH 6.0 and subsequent neutralization cannot restore lost infectivity. However, viability, of virus producer cells is hardly affected between pH 7.4 and 4.9, but their ability to release infectious particles is lost gradually, similarly to the case of cell-free viruses. Neutralization of the media after treatment results in gradual restoration of releasing infectious viruses. These data explain that, cell-free HIV-1 looses infectivity in the acidic vagina or does on the skin, but infectivity is preserved in the blood, semen, rectum and breast milk being neutral or slightly alcalic. Virus carrier or producer lymphocytes by any route of infection can survive such protective mechanism of the bod