The expression of BMP4 and FGF2 in Wistar rats (Rattus norvegicus) post application of gourami fish (Osphronemus goramy) collagen

Background: Periodontitis is a chronic inflammatory disease of the periodontal tissue that is characterized by alveolar bone resorption. This occurs due to an imbalance of osteoblast and osteoclast during the bone formation and resorption processes. In order to obtain complete regeneration of periodontal tissue, bone grafting is frequently used in periodontal surgical therapy. Although each material has disadvantages, safe graft materials derived from animal sources can be employed as an alternative to bone graft materials. Osteoblast, osteoclast, calcified bone matrix, type I collagen, osteonectin, and hydroxyapatite can all be found in gourami scales, a form of food waste. BMP4 has osteoinduction functions, which are important in bone metabolism. Through angiogenic activity, FGF2 also contributes to periodontal regeneration. Purpose: The aim of the study was to assess the expression of BMP4 and FGF2 after the treatment group had been given gourami fish scale extract. Methods: Thirty-two experimental three-month-old male Wistar rats (150-200g) were randomly divided into four groups: a seven-day control group, a seven-day treatment group, a 14-day control group, and a 14-day treatment group. One mandibular incisor was extracted from each Wistar rat. The post-extraction socket was filled with blood for the control group and collagen extract for the treatment. Results: The one-way ANOVA test showed a significance level of 0.000 (p = <0.05). Conclusion: The expression of BMP4 and FGF2 increased after the application of collagen extract from gourami scales

Gingivectomy Related To Conservative Treatment: A Case Report

Gingivectomy is the excision of gingival tissue, usually to remove the diseased wall of a periodontal pocket (true pocket or pseudopocket). The main indication for gingivectomy is fibroblastic gingival enlargements. Gingival enlargement also known as gingival overgrowth is defined as increase in the size of gingiva. Gingival enlargement can be caused by a wide variety of etiologies. Plaque-induced inflammation can be the sole cause of gingival enlargement or can be the secondary cause. Gingival enlargement can be a functional and esthetic disparity so therapy to control gingival enlargement is essential. In this presentation a 20̻years̻old, systemically healthy, non̻smoking female presented with the complaint of bleeding gums and gingival enlargement in upper fractured teeth causing un̻aesthetic appearance. Improvement of facial esthetics contributed to the self̻confidence of an adult periodontal patient with pathologic gingival enlargement

OPG and RANKL Signal Transduction in Osteoblast Regulation Post Application Extract Collagen in Osteogenesis

Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneration. Important factor for periodontal regeneration is to promote bone formation, nowadays used bone replacement. The used of xenograft materials as gold standard for periodontal tissue regeneration using type I collagen bone graft has been widely developed. The main organic component in fish scales is type I fibril collagen, which are same as with component in bones. Specific markers of bone formation are the expression of osteoblast, osteoclast, osteoprotegerin (OPG), and receptor activator of nuclear factor κB ligands (RANKL). The purpose of this study is to assess the expression of OPG and RANKL after application of extract collagen from gourami (Osphronemus gouramy) scales. Materials and Methods: Thirty-two experimental 3-month-old male Wistar albino rats (150g and 200g) were randomly divided into four groups: 7-day control group, 7-day fish collagen group, 14-day control group and 14-day fish collagen group. The sockets were filled with blood (control group), whereas 10 mg to 30 mg extract collagen was added until the sockets were fully occupied (treatment group). Results: The ANOVA test showed a significance level of 0.000 (p <0.05). Conclusion: Expression of OPG enhanced and expression of RANKL lowered after application of type I collagen from gourami scales, accelerating osteogenesis