Additional file 1: of Evidence of suppression of onchocerciasis transmission in the Venezuelan Amazonian focus

Detailed description of study area, criteria for elimination, calculation of infection indices and additional entomological data. Text S1. Geographical and environmental characteristics of the Venezuelan part of the Amazonian onchocerciasis focus. Text S2. Endemic communities, mapping and geographical information system. Text S3. World Health Organization (WHO) criteria for onchocerciasis elimination. Text S4. Calculation of parasitological indices. Text S5. Calculation of transmission indices. Table S1. Number of ivermectin rounds for twice yearly (6-monthly) and quarterly (3-monthly) treatments that reached a therapeutic coverage ≥85 %, by geographical sub-area in the Venezuelan part of the Amazonian focus. Figure S1. Monthly biting rates of Simulium guianense s.l. recorded in Orinoquito and Parima B during baseline (1982–2000) entomological evaluations. Figure S2. Infectivity rates and mean nos. of L3 larvae per fly for S. guianense s.l. in Orinoquito and Parima B during baseline (1982–2000) entomological evaluations. Figure S3. Monthly infective biting rates and monthly transmission potentials for S. guianense s.l. in Orinoquito and Parima B during baseline (1982–2000) entomological evaluations. Figure S4. Relationship between the proportion of infective flies and the mean number of infective larvae per fly in S. guianense s.l. at baseline. Table S2. Comparison of manual dissection and PCR (using PoolScreen™) for estimation of infectivity rates in S. guianense s.l., Koyowë, May 2000. (DOCX 129 kb