DNA barcodes and new primers for nature's pest controllers: the social wasps

Globally, biodiversity is declining because of anthropogenic pressures, and this could lead to extinction of some species before they are discovered. The loss of insect taxa is of prime concern, given recent reports of significant declines in the populations of many taxa across the globe. Efforts to document biodiversity have met with several challenges, amongst which are the difficulties in using morphological features to discriminate species, especially in insects. DNA barcoding is a rapid and reliable method for species identification and discovery but choosing appropriate primers to amplify the barcode region without co-amplifying contaminants remains a key challenge. We developed and tested a set of primers for PCR amplification of the DNA barcode region of the COI gene in polistine wasps.Zethus miniatus Saussure. Samples were obtained from Africa, Americas, Asia, and Europe. The polistine-specific primers successfully amplified the barcode region for all polistines tested, without amplifying any Wolbachia present; they also worked with many species from the other Vespidae wasp subfamilies. The new primers are valuable for the discovery and accurate documentation of polistine wasps in the four continents

Poverty Level Between Remittance-Receiving and Non-receiving Households and the Effect of Poverty on Different Zones, Quintiles, Sex, and Sectors in Nigeria

This study examines the difference in poverty level between remittance-receiving and non-receiving households and the effect of remittances on different zones, quintiles, sex, and sectors in Nigeria, using household data from the Nigerian National Living Standard Survey. A linear regression model was employed for estimating counterfactual per capita household expenditure without remittance, while Probit regression model was adopted to examine the effect of remittances on poverty, to know the difference between remittances receiving and non-receiving households. In other to correct for selection bias that could lead to an inconsistent estimate, since migrants were not randomly selected from the pool of households, Heckman selection model,  –  two-step estimates were used. Empirical results showed that poverty level between remittance-receiving and non-receiving households differ. Remittances led to poverty reduction in households. A mixed result was, however, found on the effect of remittances on different zones, quintiles, sex, and sectors. On the basis of the above, the study recommended that remittance-receiving households do not over depend on remittances and working less, which could negatively affect the development of their own financial and economic resources, despite the evidence that remittances contribute to poverty reduction. The government can as well facilitate and encourage expatriate Nigerians in sending remittances to their households. In respect to this, issuance of remittance bonds would be a right step. Keywords: Poverty, Remittance

Load frequency control using classical controllers

This paper presents a solution to the problem of load frequency control of a two area power system using PI and PID controllers. In electric power systems, the load demand varies at different times of the day and these load variations lead to changes in frequency of the power system. In an interconnected power system, the tie-line power also varies in addition to the frequency variations. These changes in frequency and tie-line power causes instability in the power system network and make it unreliable. Load frequency control therefore ensures that these changes in the tie-line power and frequency of the control areas are minimized with acceptable level of overshoot and settling time. The method used involves the modeling of the two area interconnected power system using Matlab/Simulink software, the PI and PID controllers were then tuned using the Ziegler-Nichols tuning rule and used to simulate the two area power system when a load change of 0.2pu occurred in control area one. The simulation results showed that both the PI and PID controllers were able to restore the changes in tie line power and frequency of the control areas caused by the change in load to their steady state values of zero but the PID controller has better dynamic performances of overshoot, rise time and settling time than the PI controller

Process optimization of resources for packaged water factories in Nigeria

Part of: Seliger, Günther (Ed.): Innovative solutions : proceedings / 11th Global Conference on Sustainable Manufacturing, Berlin, Germany, 23rd - 25th September, 2013. - Berlin: Universitätsverlag der TU Berlin, 2013. - ISBN 978-3-7983-2609-5 (online). - http://nbn-resolving.de/urn:nbn:de:kobv:83-opus4-40276. - pp. 108–113.Inaccessibility to drinking water is an intractable growing problem in developing countries such as Nigeria. This paper, presents the energy and manpower input resources needed to increase water accessibility and guarantee sustainable profitable operations. The work relied on detailed questionnaire administration for data collection from water packaging factories within Nsukka and Enugu Cities. The data were collated and Project Evaluation Review Technique (PERT) was used to determine the amount of energy needed. A profit profile was determined for both sachet and bottle water products. The gross energy sequestered by the packaging process is 87.8J for sachet water and 0.52 MJ for bottle water with average of 10 workforces. Also, optimal production rates of 1658 and 1551 were determined for sachet and bottle water, respectively at a profit of N 291,428.29 per day. The results have significant implications for Nigeria’s millennium development goals target for water in 2015

Outcomes of cleft palate surgeries at the National Orthopaedic Hospital, Enugu, Nigeria: November 2008 – November 2013

Background: Despite an increase in the number of palatoplasty procedures at the National Orthopaedic Hospital Enugu (NOHE) sequel to a partnership with Smile Train, no reports on subsequent outcomes have been published. We investigated the speech outcomes and rates of fistula formation, the relationship between introduction of solids and incidence of post-operative oronasal fistulae and the benefits of post-operative honey licks. Objective: To determine the outcome of palatal repairs performed at our center in relation to the timing and nature of post-operative feeds. Method: This was a cohort study of patients who had palatoplasty over a five-year period and were subsequently followed up for a maximum period of 9 years. The patient’s present condition, timing of first feeds, onset of solid feeds, honey licks, frequency of wound dehiscence, fistula formation, and speech outcomes were assessed. The evaluation for a fistula was made from two weeks after the surgery by a senior resident in plastic surgery. Analysis was done using SPSS version 21.0 and p value set at <0.05. Results: A total of 115 surgeries: 90 primary cleft palate repairs, 6 combined cleft lip and palate surgeries and 19 secondary cleft palate repairs were done. Male to female ratio was 1:1.3. Age range of patients was 6 weeks to 36 years. Timing of introduction of solid meals significantly affected incidence of repair breakdown; and 58% had normal to near-normal speech. Conclusions: Licking honey was associated with fewer wound breakdowns. Early return to solid feeds is associated with a higher incidence of wound breakdown following palate repair

Segmentation in Agriculture and Farmers’ Productivity: Evidence from Survey of Poultry Farms

The World Bank intervened in Nigeria agriculture in 2009 to increase productivity in 5 farm areas, namely-poultry, rice, maize, cashew and pineapple farming through the value chain strategy. Agricultural activity was segmented and farmers were encouraged to go into one farm operation only. Proponents of the segmentation argue that it holds the key to solving the low agricultural productivity in developing counties because farmers will only specialize in one farm area. That is, specialization will enable the farmer to innovates better ways of tackling problems, time the market and planting period to mitigate climate change. The aim of the research is to examine the effects of the segmentation practice on the productivity of Poultry farmers in Nigeria, using a survey data. Result from the survey using regression analysis did not support encouraging segmentation in poultry farming. This is because poultry farmers who engaged in more than one poultry practice had more yield of egg per bird. The lesson for agricultural policy is that farmers should be encouraged to engage in multiple farming practice

Effect of early amniotomy on the outcome of spontaneous labour: a randomized controlled trial of pregnant women in Enugu, South-east Nigeria

Background: Early amniotomy is common in obstetric practice but, its effectiveness has not been proven.Objectives: To determine the effects of early amniotomy on the duration of labour, and other maternal / neonatal out- comes of uncomplicated pregnancies in Enugu, South-east Nigeria.Methods: A randomized controlled study of 214 consenting term pregnant women at the University of Nigeria Teaching Hospital Enugu, Nigeria. Intervention group received amniotomy early in active labour while the control group had their membranes conserved.Results: Mean duration of labour for the amniotomy group (279.4 ± 53.7 minutes) was significantly lower than that of con- trol group (354.4 ± 67.5 minutes), (t = -8.988, p <0.001). Three (3.8%) women in amniotomy group needed oxytocin aug- mentation as against 21 (19.6%) women in the control group RR = 0.14, (CI 95%: 0.04 – 0.46), NNT = 16. The two groups did not vary with respect to cesarean section rate, newborn Apgar scores, and need for new born special care unit admission. Conclusion: Early amniotomy when compared to fetal membrane conservation reduced the duration of labour and need for oxytocin augmentation among term singleton pregnant women in Enugu, Nigeria. Its routine use in well selected cases may reduce prolonged labour and its complications.Keywords: Early amniotomy, duration of labour, oxytocin augmentation, term pregnancy, caesarean section rate, Enugu- Nigeria

Patterns of Antimicrobial Use in a Specialized Surgical Hospital in Southeast Nigeria: Need for a Standardized Protocol of Antimicrobial Use in the Tropics

Background: Antimicrobial resistance remains a growing global health menace. One of the key actions to curb this menace by the World Health Organization is antimicrobial stewardship (AMS). A prescription protocol is one of the cost‑effective AMS interventions in surgery. This study determines the patterns of antimicrobial usage in a hospital specialized in orthopedic and plastic surgeries care in Nigeria. Methods: A cross-sectional survey was carried out at National Orthopaedic Hospital Enugu, a tertiary hospital specialized in orthopedic and plastic surgeries in Southeast Nigeria in May 2019. All the inpatients were included in the study. A standardized tool for point prevalence survey was used to collect data. Data were analyzed using Epi Info version 7.2.4. Results: A total of 127 inpatients  participated in the survey with 387 antimicrobial encounters. The most common reasons for antimicrobial use were for the treatment of community-acquired infections (65.0%) and prophylaxis (29.4%). The decision for their use was made majorly on an empirical basis (92.4%). The reasons for antimicrobial prescriptions were documented in the majority (97.5%) of the cases and stop review dates in all (100%) of the prescriptions. Ceftriaxone (25.7%), tinidazole (21.9%), and metronidazole (14.6%) were the commonest antimicrobials prescribed among the patients. Conclusion: Orthopedic and plastic surgery practices require tailored prophylactic antibiotic regimens in the tropics due to peculiarities of both the specialties and the subregion. The claim that existing protocols in the temperate regions may apply in the tropics has been questioned due to the microbial profile on the tropics. Keywords: Antimicrobial protocol in surgery, antimicrobial resistance, antimicrobial stewardship, prophylactic antibiotic