Assessment of Thyroid Function in Infertile Iraqi Females

Infertility is one of the medical, social and psychological burdens in Iraqi society. Thyroid dysfunction can lead to menstrual disturbance, anovulatory cycle and decreased fertility. The study was designed to evaluate the role of thyroid disorders in infertility with emphasis on autoimmunity by Measuring T3, T4, TSH, & anti Thyroid peroxidase antibody in infertile females comparing the results with matching fertile controls. The study was conducted during the period from March 2015 to September 2015 at Karbala Maternity Hospital, infertility unit, and some private clinics. This study included a total number of 143 women in the reproductive age; ranging between (15- 43) years; divided into: The patients group included 92 infertile females; while controls were 51fertile females. The following parameters were measured for all study groups: T3, T4, TSH, &anti-TPO using ELISA device. The results showed that there were 60.8% of patients with primary infertility (n =56) while 38.2% were with secondary infertility (n= 36). There was a significant difference in T3, and Anti-TPO between patients and controls being higher in patients; (p-value <0.05). In addition; Anti-TPO level was significantly higher in secondary infertile patients compared to primary infertile patients. In conclusion; Thyroid disorders are closely related to infertility in Iraqi females; and Anti-TPO may be used as a biochemical indicator of subclinical thyroid disorders and may help in assessment of thyroid function as a cause of infertility whether primary or secondary. Keywords: Infertility, Thyroid disease, T3, T4, Anti-TP

Investigating the anti-carcinogenic potential action of 1,2,3 triazole core compounds: impact of introducing an aldehyde or Nitro group, integrating cell line studies, and in silico ADME and protein target prediction

The development of novel chemotherapeutic drugs begins with the suppression of cancer and angiogenesis. Ringed compounds with one or more heteroatoms are known as heterocyclic compounds. In organic chemistry and the pharmaceutical sector, heterocyclic compounds containing nitrogen atoms are valuable. In pharmaceutical chemistry, molecules containing a 1,2,3-triazole skeleton are particularly favored. They have great stability, making it simple to bind them to biomolecular targets. In this work, two 1,2,3-triazole scaffolds containing new chemical entities were assessed using the MTT assay against two malignant (MCF-7 and HCT116) and one normal (HUVECs) cell lines with the goal of creating a new leading prodrug for cancer treatment. The ligands were well characterized by FTIR and 1HNMR. In silico ADMET studies show acceptable pharmacokinetic properties. With the aid of the ligands’ SWISS target protein prediction, the in silico binding to target proteins was examined. The two compounds exhibited a dose-dependent cytotoxic effect, with the H4 compound demonstrating a favorable selectivity index against MCF-7 breast cancer, indicating its potential as a leading compound for anticancer prodrugs

Phytochemical Screening of leaves of Polyalthia sclerophylla using Classical Methods and GC-Mass Spectroscopy: Cytotoxicity and Antibacterial Activities

Background: Polyalthia sclerophylla (P. sclerophylla) is a member of the Annonaceae family, with a wide distribution in tropical and subtropical regions. In traditional Chinese medicine, various members of this genus have been employed as medicinal plants to address refractory ailments. The current study aims to extract the leaves of Polyalthia sclerophylla (TLPS) and determine their chemical contents using GS-Mass and standard phytochemical techniques.Methods: The antibacterial and cytotoxicity activities of TLPS were used to evaluate its bio-medical characteristics. In that sequence, three solvents were used to extract TLPS to produce three samples: MTLPS, DTLPS, and HTLPS.Results:  Phytochemical analysis indicated terpenoids and glycosides in all prepared models, but no alkaloids were observed. The GC-Mass data showed twenty-one chemical compounds. Pyridine, 2-Undecanol, 2-methyl-2-oxiranyl- and cyclobutanone were observed with higher percentages, while the 2(5H)-Furanone, 5-methyl, 1,1,3-Trimethylcyclopentane and 1,6-Heptadiene were observed with lower rates. The cytotoxicity study of TLPS was performed using Alamar blue assay using MG-63 cells. However, results show no detrimental influence at any dosage as cell availability increased. MTLPS, DTLPS and HTLPS were treated with six bacteria pathogens, and all of them showed significant effects against these bacteria to inhibit the growth of the bacteria. The extract of methanol (MLPS) was more effective in inhibiting the development of the bacteria compared with the DTLPS and HTLPS.Conclusion: The current work has shown that the chemical composition of crude TPLS varies, leading to notable chemical, biological, and medicinal features, and their non-toxic impact.Keywords: Polyalthia sclerophylla; GS-Mass; Antibacterial; Cytotoxicity activity

Evaluation of the oocyte quality versus ICSI outcomes in sub fertile Iraqi women with polycystic ovary syndrome

Background: Polycystic ovarian syndrome (PCOS) is a common endocrine condition, occurs in about 5% to 10% of women and is thought to be caused by a hypothalamic disorder, In some women with PCOS especially obese one has hyper secretion of insulin with insulin resistance, the aim of study: To determine oocyte number and quality and ICSI outcome in infertile patient with PCOS. All patient fit with diagnostic criteria of PCOS where undergoes stimulation by antagonistic protocol and ICSI procedure is done under a control condition. The result of pickup (number and quality of oocyte) and result of ICSI (number of 2 pronclea), was compared with result of oocyte pick up and ICSI of tubal factor.  Result and Discussion: In this study characteristic clinical feature of PCOS like acne, hirsuitism, irregular cycle not found in tubal factor group. Also some hormones like LH, Testosterone and prolactin levels in PCOS higher than tubal factors group with normal ovaries As a result of ovarian stimulation and ICSI procedure shows There is good number of oocytes and good quality (M2), with higher fertilization rate in patient with PCOS undergoes ICSI than patient with normal ovaries