Tropical Diabetic Hand Syndrome

Any adult with diabetes in the tropics with hand cellulitis, infection and gangrene qualifies for tropical diabetic hand syndrome (TDHS). We reviewed a 39‑year‑old woman with a 3‑week history of swelling of the left index finger following an insect bite. The swelling progressively increased in size, was very painful, and extended to the palm. There was no history or symptoms suggestive of chronic complications of diabetes. Random blood sugar on presentation was above 600 mg/dl using a glucometer. Examination revealed an edematous left palm draining pus from multiple sinuses, necrotic and gangrenous left index finger extending down to just above the thenar eminence. A diagnosis of TDHS in a patient with hyperosmolar state was made. She was managed accordingly and subsequently underwent aggressive debridement and desloughing. Two fingers were amputated and the wound was allowed to heal by secondary intention.Keywords: Diabetes, Hyperglycemia, Nigeria, Tropical diabetic hand syndrom

Breed and gender effects on blood profile of Muturu and Bunaji cattle in Benue and Ogun State, Nigeria

This research was conducted to study the blood profile of Bunaji and Muturu cattle in Benue and Ogun States. Four hundred and eighty cattle comprising 240 of each breed and 120 of either gender at each location were sampled. The experiment was set in a 2×2×2 factorial format in a CRD with location, breed and gender as factors. Samples were collected five times at each location. Blood parameters analyzed were PCV, Haemoglobin (Hb), Red Blood Cell Count (RBC), Lymphocytes, Granulocytes and Monocytes. The results showed that Muturu cattle presented significantly (p<0.05) higher mean PCV, Hb, WBC, percent Lymphocyte and Monocytes. It was also observed that Muturu cattle at Benue State showed higher (p<0.05) mean PCV, RBC and percent granulocyte while the Bunaji presented higher (p<0.05) mean MCHC, with the bulls presenting significantly (p<0.05) higher mean MCHC (39.45g/dl) than the cows (36.38g/dl). Similarly, higher mean MCHC were observed in Muturu bulls (31.02g/dl) compared to the cows (28.90g/dl) of Benue State. These variations in mean MCHC were not observed among cattle in Ogun State. Mean lymphocytes and granulocytes varied significantly (p<0.05) with Bunaji gender in Ogun State. The study concluded that location and breed affected haematological parameters of the cattle breed investigated. Key words: Blood; Breed; Bunaji; Gender; Mutur

Detection and typing of Human Papillomavirus in urine from patients attending a sexually transmitted infections clinic in Nairobi County, Kenya

Human papillomavirus (HPV) is a common sexually transmitted infection (STI) that has been etiologically linked to cervical cancer. Different types of samples can be used for cervical screening, including Pap test or biopsy and Liquid Based Cytology, visual inspection using acetic acid or Lugol’s iodine, and HPV testing. These methods are invasive. The use of urine as an alternative specimen may be more widely accepted since it is non-invasive and the sample is readily available. The study aimed at detecting and genotyping HPV in urine from patients attending a sexually transmitted infections clinic in Nairobi County. It also aimed at assessing the factors associated with HPV infection. In this cross-sectional study, a structured ‘risk factor’ questionnaire was administered and HPV from urine specimen was genotyped using the L1 gene. Phylogenetic and molecular evolutionary analyses were conducted. Bivariate analysis and Pearson’s chi square (χ2) tests were used to determine the association between HPV infection and factors associated with HPV. A total of 222 adults (45 males and 177 females) aged 18-49 years were recruited. The prevalence of HPV among males and females was 22.2% (10/45) and 32.8% (58/177) respectively. The prevalence of high-risk types among males and females was 25% (1/4) and 27.5% (11/40) respectively. The high risk HPV genotypes detected among females were: HPV-16 (10%), -66 (7.5%), and -70 (7.5%) while low risk types were HPV 6 (27.5%), followed by -81 (25%), -83 (10%), -11 (7.5%), and -54 (2.5%) respectively. The prevalence of low risk types among males and females was 75% (3/4) and 72.5% (29/40) respectively. The prevalent low-risk HPV type detected in males was HPV type 6 (75%) while HPV-58 (25%) was the only high risk type in males. History of sexually transmitted infections was significantly associated with HPV infection among females (P=0.002). There was also significant association between marital status among males (p=0.046), how often one had used the contraceptives among females (p=0.038) and HPV genotypes at bivariate level. The results indicate high HPV prevalence, high risk and low risk HPVs could be detected in urine from the two populations. Therefore; molecular testing of HPV on urine samples is a method that utilizes a non-invasive technique that may increase screening coverage as it is easy to obtain. Key words: urine, Human papillomavirus, HPV genotypes, PCR, cervical cancer