Oral Health Literacy and COVID-19 Pandemic Anxiety and Concerns Among Iranian Orthodontic Patients

Objective: This study aims to evaluate the impact of the COVID-19 pandemic on orthodontic appointments, and the concerns and anxiety levels of orthodontic patients in Iran.Methods: An anonymous 40-question questionnaire was sent online to the patients of private orthodontic clinics in 2 cities of Birjand, and Mashhad, capitals of Eastern provinces of Iran. Orthodontic patients of Mashhad dental school received the questionnaire as well. Descriptive statistics were reported, and the association between gender, oral health literacy (OHL), and adherence to orthodontic appointments and attendance, along with data on feeling, concern, and level of anxiety regarding quarantine/coronavirus pandemic were evaluated using the chi-square analysis.Results: Three hundred twenty-four patients, with a mean age of 32.43 years, answered the questionnaire. Fifty-seven percent of participants said that they only left home for their daily requirements. Seventy-two percent of the participants reported feeling calm about the COVID-19 pandemic and its consequences. Most of the patients (74%) said that they would attend their orthodontic appointment in case of urgency, and 41% of patients declared that their greatest concern was the probability of a delay in completion of treatment. Forty-five percent of patients had adequate OHL. There was a significant association between OHL and willingness to attend orthodontic treatment, concerns regarding the impact of the pandemic on orthodontic treatment, and the frequency of daily teeth brushing.Conclusion: Women respected the quarantine more than men. Patients with higher OHL were more willing to attend their appointments, but only in case of an emergency, due to the outbreak of COVID-19. Women were more concerned and had better oral hygiene behaviors

Piezopuncture-Assisted Canine Distalization in Orthodontic Patients: Two Case Reports

With the increasing number of young adults seeking orthodontic treatment to improve their smile esthetics or oral function, the time spent wearing brackets is one of the biggest challenges for these patients. Various surgical techniques have been developed over the years to accelerate tooth movement and reduce the total treatment time. A newly introduced, minimally invasive procedure namely piezopuncture, which uses a piezosurgical tool to create multiple cortical punctures through the gingiva, is presented in this report of two extraction cases

Evaluating the Efficacy of a Modified Piezo-Puncture Method on the Rate of Tooth Movement in Orthodontic Patients: A Clinical Study

Objective:Owing to the increasing demand from orthodontic patients for a more rapid treatment, many studies have focused on accelerated tooth movement. Currently, one of the prevalent methods to achieve accelerated tooth movement is piezo-puncture. The aim of the present study was to evaluate the effect of a modified piezo-puncture method on tooth movement rate and type during canine retraction.Methods:A total of 17 patients who required fixed orthodontic treatment with extraction of the maxillary first premolars were included in the study. Following a split-mouth design, upper canines were retracted with Ni–Ti coil spring that applied 150 g of force on each side (piezo-puncture on one side and contralateral side served as the control). Then, the rates of tooth movement, canine angulation and rotation, and anchorage loss were evaluated at T0 (before the intervention), T1 (1 month after the intervention), and T2 (2 months after the intervention). For calculating the canine movement rate, either the distance between the canine and the lateral incisor or the space between the second premolar and the canine was measured. In addition, pain perception was documented by Visual Analog Scale. Data were analyzed using the Kolmogorov–Smirnov normality test, Spearman correlation test, paired sample t-test, and Wilcoxon signed-rank test.Results:No significant acceleration was observed in canine movement, canine tipping, rotation, or anchorage loss of molar in different times.Conclusion:Considering the limitations of the study, the application of piezo-puncture employing the protocol used in the present study failed to accelerate tooth movement and to decrease the unfavorable tipping, rotation, and molar anchorage loss

Is Evaluation of Hepatitis A Immunity Required or Not?

Hepatitis A virus (HAV) infection is one of the most common causes of acute hepatitis and it is a serious health problem worldwide. HAV infection is a vaccine preventable disease that can produce the lifelong immunity seen in many developed countries with the vaccination schedule administered to children; however this vaccine is not used in developing countries at the present time. Improvements in food and water hygiene have caused a displacement of hepatitis A infection from children to adults which has increased mortality rates. Therefore evaluation of HAV immunity levels can help health authorities develop polices for prophylaxis especially in developing countries

Evaluation of the effects of three different mouthwashes on the force decay of orthodontic chains

BACKGROUND: Elastomeric chains are commonly used in orthodontics. Force decay in these materials poses clinical problems. The aim of this study was to evaluate the effects of three different mouthwashes on the force decay of orthodontic chains. MATERIALS AND METHODS: In this experimental study, elastomeric chains with two different configurations were divided into eight groups (two control and six test groups). After 10 s of prestretching up to 100% of their initial length, the chains were stretched for 25 mm on jig pins and then immersed in artificial saliva, persica, chlorhexidine 0.2% and sodium fluoride 0.05% mouthwashes. Ten cycles of thermocycling between 5°C and 55°C were conducted daily during the test period. In order to reach a 200-g initial force, seven loop closed chains, and five-loop short chains were selected. Forces were recorded by digital force gauge (Lutron) at initial, 24 h, 1, 2, 3 and 4 weeks for all groups. The amount of force loss was compared among different mouthwashes and times using one-way analysis of variance (post-hoc, Tukey, α = 0.05). RESULTS: About 20% of the force decay occurred during the first 24 h, but after that and up to the 4(th) week the rate of force loss was gradual and steady. After 4 weeks, persica and chlorhexidine caused the lowest and the highest percentage of force loss, respectively. These two mouthwashes showed statistically significant differences at all points of time (P < 0.05). CONCLUSION: Within the limitations of this study, during the orthodontic treatment, persica is preferred to chlorhexidine for oral health control

Comparison of esthetics perception and satisfaction of facial profile among male adolescents and adults with different profiles

Objective: To evaluate esthetics perception and satisfaction of the facial profile among Iranian male adolescents and adults. Materials and Methods: In this cross-sectional study, male subjects referred to Orthodontic Department of Mashhad Dental School were enrolled (n = 84) and were divided into two groups: Adolescents (n = 39), and adults (n = 45). They were also assigned to straight, convex, or concave profile groups based on the facial profile angle (G-Sn-Pog′). An ideal silhouette of the lower facial profile was designed in Adobe® Photoshop® CS2 software (Kansas, USA). Then, eight other silhouettes representing different relations of the maxilla and mandible were constructed. Patients were asked to use numbers 1-10 to rank the facial profiles in the order of the attractiveness, and choose a silhouette that best closely resembled their own profile. Moreover, using a questionnaire patients were asked to rank their satisfaction with their profile, and asked to assign a number (1-5) to each question as follows; one represented the least satisfaction, while five reflected the highest satisfaction. Results: Adult and adolescent subjects with straight (adults: 12.0 ± 1.9, adolescents: 12.8 ± 1.05) and concave (adults: 10.0 ± 2.14, adolescents: 10.0 ± 2.08) profile showed the highest and the least satisfaction with their own profile, respectively. Both adult and adolescent group selected "retrognathic maxilla, prognathic mandible" as the least attractive profile. Overall, "straight" and "bimaxillary dentoalveolar retrusion" were chosen as the most attractive silhouettes in adolescent and adults, respectively. In comparison to a professional opinion (clinician ranking), 42.9% of adolescents and 22% of adults were able to correctly diagnose their own profiles type. Conclusion: Most of the male adolescents and especially adults diagnosis of their own profile differed with a professional assessment

The Effect of Different Soft Drinks on the Force Degradation of Conventional and Memory Orthodontic Elastic Chains: An In-Vitro Study

Objectives: The aim of this study was to assess how different soft drinks affect the deterioration of conventional and memory orthodontic elastomeric chains. Materials and Methods: We used 500 five-loop segments of elastomeric chains, which were divided into two equal groups of conventional and memory chains. The samples were kept in artificial saliva during the study period. Each group was further divided into 5 subgroups consisting of artificial saliva (controls), Coca-Cola®, non-alcoholic beer (ISTAK®), and carbonated and non-carbonated yoghurt-based drinks (Alis®). Treatment with the soft drinks consisted of immersion in the test liquids twice a day for 3 minutes each time. Force measurements were taken on days 1, 7, 14, 21, and 28. Data were analyzed using One- and three-way ANOVA tests, and independent t-test, with a significance level of 5%. Results: The mean initial force for the conventional and memory elastomeric chains was 3.34±0.112N and 2.49±0.209N, respectively. Conventional chains showed significantly greater degradation than memory chains (P<0.01). Soft drinks had a significant impact on force degradation for both types of chains at all time points (P<0.01). Coca-Cola® had the highest level of force degradation, while non-carbonated yoghurt-based drinks had the lowest (P<0.01). Conclusion: Based on the findings of this study, it is recommended that orthodontic patients choose non-carbonated yoghurt-based drinks during their treatment. Memory elastomeric chains may be more suitable for patients who consume large amounts of carbonated soft drinks, due to their lower amount of force degradation compared to conventional chains

Exploring non-viral methods for the delivery of CRISPR-Cas ribonucleoprotein to hematopoietic stem cells

Abstract Gene manipulation of hematopoietic stem cells (HSCs) using the CRISPR/Cas system as a potent genome editing tool holds immense promise for addressing hematologic disorders. An essential hurdle in advancing this treatment lies in effectively delivering CRISPR/Cas to HSCs. While various delivery formats exist, Ribonucleoprotein complex (RNP) emerges as a particularly efficient option. RNP complexes offer enhanced gene editing capabilities, devoid of viral vectors, with rapid activity and minimized off-target effects. Nevertheless, novel delivery methods such as microfluidic-based techniques, filtroporation, nanoparticles, and cell-penetrating peptides are continually evolving. This study aims to provide a comprehensive review of these methods and the recent research on delivery approaches of RNP complexes to HSCs