Localization and Chemical Speciation of Cadmium in the Roots of Barley and Lettuce

Plants have the potential to accumulate toxic amounts of cadmium (Cd), and understanding how and where Cd is stored in plants is important for ensuring food safety. Previous experiments have determined that a greater amount of Cd is translocated into the leaves of lettuce (Lactuca sativa) as compared to barley leaves (Hordeum vulgare). Preferential retention of Cd in root of barley would explain this difference. Hence, the purpose of this study was to determine the localization and coordination environment of Cd (i.e., the ligands to which Cd was bound) in the different root tissues of lettuce and barley using histochemical staining, electron microscopy and micro X-ray spectroscopy. Retention of Cd in barley roots could be explained by accumulation of Cd at the endodermis, comparatively higher amounts of Cd sequestered in the symplast of cortical cells and binding to xylem cell walls. Increased translocation of Cd to lettuce shoots seemed to be due to a less effective barrier at the endodermis and less sequestration of Cd in the cortex. Regardless of the tissue type, most of the Cd2+ was bound to S ligands in the roots of barley, possibly reflecting accumulation of Cd–phytochelatin and Cd–S molecules in the vacuoles. In lettuce roots, Cd was more evenly distributed among ligands containing S, O and NO3 groups, which is indicative of proportionately more Cd binding to the cell walls, relative to barley. These results will be useful in uncovering the mechanisms of differential Cd-tolerance and sequestration in lettuce and barley

Paleo-Hydrothermal Predecessor to Perennial Spring Activity in Thick Permafrost in the Canadian High Arctic, and Its Relation to Deep Salt Structures: Expedition Fiord, Axel Heiberg Island, Nunavut

Published versionIt is surprising to encounter active saline spring activity at a constant 6° C temperature year-round not far away from the North Pole, at latitude 79° 24′N, where the permafrost is ca. 600 m thick and average annual temperature is -15° C. These perennial springs in Expedition Fiord, Queen Elizabeth Islands, Canadian Arctic Archipelago, had previously been explained as a recent, periglacial process. However, the discovery near White Glacier (79° 26.66′N; 90° 42.20′W; 350 m.a.s.l.) of a network of veins of hydrothermal origin with a similar mineralogy to travertine precipitates formed by the springs suggests that their fluids have much deeper circulation and are related to evaporite structures (salt diapirs) that underlie the area. The relatively high minimum trapping temperature of the fluid inclusions (avg. ~200 ± 45° C, 1σ) in carbonate and quartz in the vein array, and in quartz veins west of the site, explains a local thermal anomaly detected through low-temperature thermochronology. This paper reviews and updates descriptive features of the perennial springs in Expedition Fiord and compares their mineralogy, geochemistry, and geology to the vein array by White Glacier, which is interpreted as a hydrothermal predecessor of the springs. The perennial springs in Axel Heiberg Island are known for half a century and have been extensively described in the literature. Discharging spring waters are hypersaline (1-4 molal NaCl; ~5 to 19 wt% NaCl) and precipitate Fe-sulfides, sulfates, carbonates, and halides with acicular and banded textures representing discharge pulsations. At several sites, waters and sediments by spring outlets host microbial communities that are supported by carbon- and energy-rich reduced substrates including sulfur and methane. They have been studied as possible analogs for life-supporting environments in Mars. The vein array at White Glacier consists of steep to subhorizontal veins, mineralized fractures, and breccias within a gossan area of ca. 350 × 50 m. The host rock is altered diabase and a chaotic matrix-supported breccia composed of limestone, sandstone, and anhydrite-gypsum. Mineralization consists of brown calcite (pseudomorph after aragonite) in radial aggregates as linings of fractures and cavities, with transparent, sparry calcite and quartz at the centre of larger cavities. Abundant sulfides pyrite and marcasite and minor chalcopyrite, sphalerite, and galena occur in masses and veins, much like in base metal deposits known as Mississippi Valley Type; their weathering is responsible for brown Fe oxides forming a gossan. Epidote and chlorite rim veins where the host rock is Fe- and Mg-rich diabase. The banded carbonate textures with organic matter and sulfides are reminiscent of textures observed in mineral precipitates forming in the active springs at Colour Peak Diapir. Very small fluid inclusions (5-10 μm) in two generations of vein calcite (hexagonal, early brown calcite we denominate “cal1” lining vein walls; white-orange sparry calcite “cal2” infilling veins) have bulk salinities that transition between an early, high-salinity end-member brine (up to ~20 wt% NaCl equivalent) to a later, low-salinity end-member fluid (nearly pure water) and show large fluctuations in salinity with time. Inclusions that occupy secondary planes and also growth zones in the later calcite infilling (deemed primary) have Th ranging from 100° C to 300° C (n = 120, average~200° C; independent of salinity), 2 orders of magnitude higher than average discharging water temperatures of 6° C at Colour Peak Diapir. Carbon isotope composition (δ13CVPDB) of the White Glacier vein array carbonates ranges from approximately -20 to -30‰, like carbonates formed by the degradation of petroleum, whereas carbonates at Colour Peak Diapir springs have a value of -10‰. Oxygen isotope composition (δ18OVSMOW) of vein carbonates ranges from -0.3‰ to +3.5‰, compatible with a coeval fluid at 250° C with a composition from -3.5‰ to -7.0‰. These data are consistent with carbonates having precipitated from mixtures of heated formational waters and high-latitude meteoric waters. In contrast, the δ18OVSMOW value for carbonates at Colour Peak Diapir springs is +10‰, derived from high-latitude meteoric waters at 6° C. The sulfur isotope (δ34SVCDT) composition of Fe-sulfides at the perennial springs is +19.2‰, similar to the δ34SVCDT of Carboniferous-age sulfate of the diapirs and consistent with lowtemperature microbial reduction of finite (closed-system) sulfate. The δ34SVCDT values of Fe-sulfides in the vein array range from -2.7‰ to +16.4‰, possibly reflecting higher formation temperatures involving reduction of sulfate by organics. We suggest that the similar setting, mineralogical compositions, and textures between the hydrothermal vein array and the active Colour Peak Diapir springs imply a kinship. We suggest that overpressured basinal fluids expelled from the sedimentary package and deforming salt bodies at depth during regional compressional tectonic deformation ca. 50 million years ago (Eocene) during what is known as the Eurekan Orogeny created (by hydrofracturing) the vein array at White Glacier (and probably other similar ones), and the network of conduits created continued to be a pathway around salt bodies for deeply circulating fluids to this day. Fluid inclusion data suggest that the ancient conduit system was at one point too hot to support life but may have been since colonized by microorganisms as the system cooled. Thermochronology data suggest that the hydrologic system cooled to temperatures possibly sustaining life about 10 million years ago, making it since then a viable analogue environment for the establishment of microbial life in similar situations on other planets

Geochemical investigation of perennial spring activity and associated mineral precipitates at Expedition Fiord, Axel Heiberg Island, Canadian high arctic

Two groups of perennial springs are observed in the Canadian High Arctic at Expedition Fiord on Axel Heiberg Island. Saline discharge (~1.3-2.5 molal) produces a variety of calcite (travertine) and gypsum-rich precipitates. Field observations, laboratory experiments, and geochemical modeling of the waters reveal that calcite precipitation is controlled primarily by CO2 degassing, but alternating light (sparite calcite crystals) and dark (amorphous micritic calcite spheres coated by organic material and trace metals) laminae are present. Evaluation of the geochemical environment in conjunction with field observations suggest that micrite layer formation may be influenced by microbial activity, although their presence has yet to be confirmed. In addition, discharge from two springs at Colour Peak leads to the precipitation of metastable calcium carbonate crystals (0.25-0.5 cm long) on terraced and steep mound slopes that form during winter months. Background literature and field observations suggest that this mineral may be ikaite (CaCO3•6H2O). The precipitation of this mineral may provide information regarding its environmental growth constraints and opportunities.Deux groupes des sources pérennes sont observés dans le Haut-Arctique au fjord Expedition sur l'île Axel Heiberg. Les eaux minéralisées (~1.3-2.5 molal) qui sortent des sources produisent une précipitation de dépôts variés et riches en calcite (travertine) et gypse. Les observations de terrain, les expériences en laboratoire et les modèles geochimiques des eaux montrent que la précipitation de calcite est contrôlée principalmente par la perte de gaz CO2 mais il y a des couches pâles (crystaux calcite sparite) et foncées (sphères de calcite micritique amorphe emrobées par materiaux organique et metaux trace). L'évaluation de l'environnement geochimique et les observations de terrain suggèrent que la production de la couche micritique pourrait être influencée par l'activité microbienne, mais la présence de microbes reste à confirmer. Les sources pérennes à Colour Peak produisent également des cristaux calcium carbonate "métastable" (0.25-0.5 cm) sur les pentes raides et en terrasses pendant l'hiver. La litérature et les observations de terrain suggèrent que ce minéral peut fournir de l'information sur les conditions environnementales de la croissance. f

Vegetation communities and summer net ecosystem CO2 exchange on western Axel Heiberg Island, Canadian High Arctic

Climate change is expected to result in the Arctic transitioning from a carbon sink to a carbon source environment, with models predicting half of the carbon stock of the upper 3 m soil layer to be released by the year 2300 (van Huissteden and Dolman 2012). However, uncertainty in latitudinal warming and changes in Arctic ecosystem functions, such as gross carbon ecosystem exchange (GEE), are poorly understood, in part a reflection of a high variability in vascular plant community diversity that is dependent upon and sensitive to physiographic controls, such as soil moisture, topography, and seasonal active layer depth (Walker et al. 2005). This heterogeneity complicates assessments of carbon fluxes on a landscape scale and how they will change in the future (Shaver et al. 2007), especially given their sensitivity to local changes in climate, such as warming and higher rates of rainfall (Bintanja 2018, Bintanja and Andry 2017). As part of the creation of a long-term ecological and environmental monitoring program at the McGill Arctic Research Station at Expedition Fiord, western Axel Heiberg Island, field-based studies in 2021-2022 of plant surveys and summer net ecosystem CO2 exchange monitoring were undertaken to:define the major vegetation communities;quantify and investigate CO2 fluxes with chambers and their analogous biophysical variables; andupscale plot level CO2 measurements to the landscape scale using high spatial resolution remote sensing data.The Expedition Fiord area is recognized as a polar oasis, with high plant species richness existing within an environment of heterogeneous physiography. At the moment, five vegetation communities have been identified (xeric dwarf shrub barren, xeric-mesic dwarf shub barren, mesic dwarf shrub tundra, cassiope heath, and sedge meadow) that varied as a function of species diversity, percent cover, soil moisture, and net ecosystem carbon exchange. Barren vegetation communities having stronger respiration fluxes (i.e., carbon source environments) while more vegetated communities have stronger photosynthesis fluxes (i.e., carbon sink environments). Landcover classification revealed with high accuracy (79.3%) that barren ground and barren vegetation communities cover a much larger area compared to wetter habitats. Upscaling summer season measured carbon fluxes based on the landcover map revealed that Expedition Fiord is a carbon source environment, with an average efflux of +94.6 g CO2/day. Ongoing work focuses on the expansion of carbon flux and subsurface monitoring locations, as well as studies of soil carbon and microbial diversity across the different land cover classifications, which will help to better resolve how soil microorganisms, plant detritus, labile organic carbon, soil moisture, slope, aspect, and bedrock geology influence CO2 fluxes throughout the summer season in this high Arctic setting

Polar endoliths - an anti-correlation of climatic extremes and microbial diversity

We examined the environmental stresses experienced by cyanobacteria living in endolithic gneissic habitats in the Haughton impact structure, Devon Island, Canadian High Arctic (75° N) and compared them with the endolithic habitat at the opposite latitude in the Dry Valleys of Antarctica (76° S). In the Arctic during the summer, there is a period for growth of approximately 2.5 months when temperatures rise above freezing. During this period, freeze–thaw can occur during the diurnal cycle, but freeze–thaw excursions are rare within higher-frequency temperature changes on the scale of minutes, in contrast with the Antarctic Dry Valleys. In the Arctic location rainfall of approximately 3 mm can occur in a single day and provides moisture for endolithic organisms for several days afterwards. This rainfall is an order of magnitude higher than that received in the Dry Valleys over 1 year. In the Dry Valleys, endolithic communities may potentially receive higher levels of ultraviolet radiation than the Arctic location because ozone depletion is more extreme. The less extreme environmental stresses experienced in the Arctic are confirmed by the presence of substantial epilithic growth, in contrast to the Dry Valleys. Despite the more extreme conditions experienced in the Antarctic location, the diversity of organisms within the endolithic habitat, which includes lichen and eukaryotic algal components, is higher than observed at the Arctic location, where genera of cyanobacteria dominate. The lower biodiversity in the Arctic may reflect the higher water flow through the rocks caused by precipitation and the more heterogeneous physical structure of the substrate. The data illustrate an instance in which extreme climate is anti-correlated with microbial biological diversity

Caves in caves: evolution of post-depositional macroholes in stalagmites

In a previous paper (Shtober-Zisu et al., 2012) we described millimeter to centime-sized fluid-free holes within the interiors of stalagmites of widely varying origin. We present here further observations of this phenomenon, using X-ray tomography, macroscopic and microscopic observation of sections of twenty-six stalagmites from various sites in North America and the Caribbean region. We can distinguish three types of cavities in speleothems: primary µm-sized fluid inclusions; mm to cm sized holes, aligned along the stalagmite growth axis which are clearly syngenetic; and µm to cm-sized holes away from the growth axis (“off-axis holes or OAHs”) deeply buried inside their host stalagmites, and cutting primary growth layers. Neither axial nor off axis holes contain fluid today. Off-axis holes appear to have been formed by internal corrosion of the calcite host, possibly enhanced by the action of bacteria which were sustained by permeation of through the body of the stalagmite of water containing dissolved organic species. A modern stalagmite from Israel is shown to contain bacteria associated with active hole formation

Microstructure variability in freshwater microbialites, Pavilion Lake, Canada

Calcite microbialites in Pavilion Lake, British Columbia, exhibit a diverse range in macro-morphology, biomass abundance, porosity, and mineral content. To evaluate the role of microorganisms in their formation, samples collected from a range of depths were examined by scanning electron microscopy (SEM) and synchrotron radiation-based micro-X-ray fluorescence (μ-XRF) spectroscopy to characterize both their outer surfaces as well as internal structures. Observed trends in both surface colonization as well as microbialite framework with increasing lake depth include decreasing microbial abundance on outer surfaces as well as increasing ratios of carbonate:biomass in the microbialites. Microscopic investigations of the interiors show bacteria and algae entrapped within calcite, with this calcite exhibiting micropores and casts similar in size and shape to microorganisms. Based on these observations, it is hypothesized that microbialite development in Pavilion Lake initiates calcite precipitation in phototrophic microbial mats, i.e., combined phototrophy and heterotrophy, followed by heterotrophic oxidation of organic matter leading to eventual carbonate infilling of the microbial-mineral matrix. In addition, an observed shift from cyanobacteria to algae with increasing lake depth suggests variability in contemporary conditions controlling microbialite growth and diagenesis. High photosynthetic growth rates at shallower depths result in significant porosity and friability due to biomass accumulation outpacing carbonate precipitation. At intermediate depths, lower light levels and slower growth rates of phototrophs lead to a greater proportion of the microbialite matrix being in-filled by carbonate. Carbonates precipitate initially within the bacteria-EPS matrix, with abundant uncalcified algae maintaining microbialite porosity. In the deepest waters, the presence of only sparse algal colonization as well as fine-grained, laminated metal-rich sediments covering microbialites suggests that present-day insolation levels are too low to support the development of photosynthetic microbial mats. As a consequence, heterotrophic carbonate precipitation has progressively in-filled these microbialite interiors to create lithified calcite fabrics that exhibit minimal porosity but preserve the casts of microorganisms as biosignatures. While the origin of microbialites in Pavilion Lake remains unknown, current observations provide valuable information in evaluating how environmental conditions influence microbialite growth in a freshwater, lacustrine environment