Performance, haemato-biochemical indices and antioxidant status of growing rabbits fed on diets supplemented with Mucuna pruriens leaf meal

[EN] The effects of dietary Mucuna pruriens leaf meal (MLM) supplementation on rabbits’ performance, haemato-biochemical indices and antioxidant status outside their thermal neutrality zone (21 to 25°C) were evaluated. One hundred and twenty 35-d old crossbreed (Chinchilla×New Zealand) rabbits weighing 694±5 g were allotted to 4 treatments (30 rabbits/treatment; 3 rabbits/replicate). A basal diet (crude protein: 16.9%, crude fibre: 17.6%, digestible energy: 2671 kcal/kg) was divided into 4 equal portions i.e. diets 1, 2, 3 and 4, supplemented with 0, 4, 8 and 12 g MLM/kg, respectively, and pelleted. The average body weight in rabbits fed on diets 3 and 4 was higher compared to those fed on diet 1 (control) at 91 d of age (+228 and +262 g, respectively; P=0.01). Within 35 to 91 d, the average daily weight gain in rabbits fed on diets 3 and 4 was higher compared to those fed on the control diet (+4.1and +4.8 g/d, respectively; P=0.01). The dressing-out percentage of rabbits fed on diets 3 and 4 increased (P=0.05) compared to those fed the control diet. At 63 d and 91 d of age, the white blood cell level of rabbits fed on diet 4 increased significantly compared to those fed the control diet (+5.05×109 and +5.32×109/L, respectively). At 63 and 91 d of age, the cholesterol level of rabbits fed on diets 3 (–1.0 and –1.16 mmol/L, respectively) and 4 (–1.10 and –1.21 mmol/L, respectively), were significantly lower compared to those fed on the control diet. The aspartate aminotransferase (AST) concentration in rabbits fed on diet 4 was reduced compared to those on control diet at 63 d of age (–33.68 IU/L; P=0.02). At 63 d and 91 d of age, compared to control, the activities of glutathione peroxidase in rabbits fed on diets 3 (+35.77 and +49.09 mg protein, respectively) and 4 (+54.52 and +55.02 mg protein, respectively) increased significantly, while catalase activities in rabbits fed diet 4 (+217.7 and +209.5 mg/g, respectively) also increased significantly. It could be concluded that dietary MLM supplementation enhanced the rabbits’ performance, reduced serum AST and cholesterol and improved the antioxidant status.Oloruntola, OD.; Ayodele, SO.; Adeyeye, SA.; Agbede, JO. (2018). Performance, haemato-biochemical indices and antioxidant status of growing rabbits fed on diets supplemented with Mucuna pruriens leaf meal. World Rabbit Science. 26(4):277-285. doi:10.4995/wrs.2018.10182SWORD277285264Adekonla A.Y., Ayo J.O. 2009. Effect of road transportation on erythrocyte osmotic fragility of pigs administered ascorbic acid during the harmattan season in Zaria, Nigeria. J. Cell Anim. 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Processed cocoa pod husk dietary inclusion: effects on the performance, carcass, haematogram, biochemical indices, antioxidant enzyme and histology of the liver and kidney in broiler chicken

Abstract Background In a 42-day feeding trial, the effects of processed cocoa pod husk (PCHM) inclusion in a broiler chicken diet were assessed. Methods This experiment was conducted between December 2017 and January 2018. Cocoa pod husk was collected and processed by ash treatment and rumen liquor fermentation to form a processed cocoa pod husk (PCHM). Three experimental diets were formulated at both the starter and finisher phases, in which PCHM was included at 0, 4 and 8% and designated as diets 1, 2 and 3, respectively. One hundred and eighty 1-day-old Arbor Acres broiler chicks were randomly distributed to three dietary treatments (10 birds/replicate; 60 birds/treatment) in a completely randomized design. The growth performance, carcass, relative internal organ weights and haemato-biochemical indices were determined. Histological examination of the liver and heart samples was also determined. Results The PCHM inclusion did not affect (P > 0.05) the performance characteristics of the broiler chicks, except for the feed intake that significantly (P  0.05) across the dietary treatments. The serum glutathione peroxidase and catalase concentration were higher (P < 0.05) in birds fed PCHM-inclusive diets compared to those fed the control diet. Similar histological myocardiac cell appearances were observed among the birds across the various dietary treatments. Sections show the myocardium composed of the cardiac muscle with peripherally placed nucleus separated by a defined interstitium that is free of inflammatory cells and collections. In the birds fed diet 2 and 3, histological variations observed were marked vascular congestion and perivascular inflammatory cells infiltrations in the hepatic tissue and marked infiltration of polymorphonuclear cells around the vessels and activation of hepatic macrophage: Kupffer cells. Conclusion Dietary PCHM inclusion up to 8% supports the performance, stability of haemato-biochemical indices and improved antioxidant status of the broiler chickens under heat stress condition. Histological changes were observed in the broiler chicken liver

Effect of pawpaw leaf meal and multi-enzyme supplementation in the diet on performance, digestibility, and oxidative enzyme status of rabbits

Abstract Background This study aimed at determining the effect of pawpaw leaf meal (PLM 26.14% crude protein [CP] and 16.06% crude fiber [CF]) and multi-enzyme (m-E: amylase, beta-glucanase, phytase, cellulase, and xylanase, protease) supplementation in diets on performance, nutrient digestibility, and oxidative status of rabbits. Four experimental diets were formulated and designated as diet 1(0%PLM-m-E), diet 2(0%PLM + m-E), diet 3(10%PLM-m-E), and diet 4(10%PLM + m-E). One hundred and twenty (120), 5-week-old rabbits of cross breed rabbits (Chinchilla x New-Zealand white) of equal sexes; weighing 738.21 ± 33 g were randomly distributed to 4 dietary treatments (30 rabbits/treatment; 3 rabbits/replicate). Results Highest weight gain being recorded in rabbits fed diet 4 is similar to the weight gain recorded for rabbits fed diets 2 and 3 but higher (P < 0.05) than weight gain of rabbits fed diet 1. Best feed conversion ratio (FCR) recorded in rabbits fed diet 4 was comparable to the FCR of rabbits fed diets 2 and 3 but higher (P < 0.05) than FCR of rabbits fed diet 1. Higher CP digestibility recorded in rabbits fed diets 2 and 4 were similar to CP digestibility in rabbits fed diet 3 but higher (P = 0.01) than CP digestibility in rabbits fed with diet 1. The starch digestibility was higher (P < 0.05) in the rabbits fed m-E supplemented diets 2 and 4. Higher (P < 0.05) glutathione peroxidase and superoxide dismutase activities were recorded in rabbits fed with diets 3 and 4. Conclusion PLM inclusion and m-E supplementation improved performance, crude protein, and starch digestibility and antioxidant enzyme status of the rabbits