Evaluation of Morphogenesis and Yield of Three \u3ci\u3ePennisetum purpureum\u3c/i\u3e Varieties in South-West, Nigeria

This experiment, conducted at the Federal University of Agriculture, Abeokuta, Nigeria evaluated the morphogenesis of three varieties of Pennisetum purpureum used in this study, which were the local, purple, and FI variety at different stages of growth. The plants were established through vegetative plant parts (stems) sourced from a previously established plot and planted using 1 m × 1 m spacing on plots measuring 4 m × 5 m. The plants were fertilized with split doses of poultry manure at 4 t/ha (equivalent to 120 kg N/ha). The experiment was laid out as a Randomized Complete Block Design. Data on the morphogenic traits and dry matter yield were collected at bi-weekly from the 4th week for a period of twelve weeks. Results showed that the morphogenic plant traits differed significantly (p\u3c 0.05) between the varieties, except the tiller appearance rate (p\u3e 0.05) which was not significantly (p\u3e 0.05) different when the varieties were compared. The purple variety had the least plant elongation and leaf appearance rate values, however, for both traits, the F1 hybrid and local variety had similar trait values. The F1 variety had the least (p\u3c 0.05) leaf elongation rate value and the highest leaf (p\u3c 0.05) expansion rate value. On the other hand, the local and purple variety had comparably higher leaf elongation rate and leaf expansion rate values. The purple variety had higher (p\u3c 0.05) dry matter yield at the 6th and 8th week, but at the 10th and 12th week, the local variety produced the highest dry matter yield. In conclusion, the varieties had distinct morphogenic traits. Also, while the purple variety produced higher dry matter yield at the early stages of growth, dry matter production was higher for the local variety at more advanced stages of growth (10 and 12 weeks)

A population-based estimation of maternal mortality in Lagos State, Nigeria using the indirect sisterhood method.

BACKGROUND: Pregnancy and delivery deaths represent a risk to women, particularly those living in low- and middle-income countries (LMICs). This population-based survey was conducted to provide estimates of the maternal mortality ratio (MMR) in Lagos Nigeria. METHODS: A community-based, cross-sectional study was conducted in mapped Wards and Enumeration Areas (EA) of all Local Government Areas (LGAs) in Lagos, among 9,986 women of reproductive age (15-49 years) from April to August 2022 using a 2-stage cluster sampling technique. A semi-structured, pre-tested questionnaire adapted from nationally representative surveys was administered using REDCap by trained field assistants for data collection on socio-demographics, reproductive health, fertility, and maternal mortality. Data were analysed using SPSS and MMR was estimated using the indirect sisterhood method. Ethical approval was obtained from the Lagos State University Teaching Hospital Health Research and Ethics Committee. RESULTS: Most of the respondents (28.7%) were aged 25-29 years. Out of 546 deceased sisters reported, 120 (22%) died from maternal causes. Sisters of the deceased aged 20-24 reported almost half of the deaths (46.7%) as due to maternal causes, while those aged 45-49 reported the highest number of deceased sisters who died from other causes (90.2%). The total fertility rate (TFR) was calculated as 3.807, the Lifetime Risk (LTR) of maternal death was 0.0196 or 1-in-51, and the MMR was 430 per 100,000 [95% CI: 360-510]. CONCLUSION: Our findings show that the maternal mortality rate for Lagos remains unacceptable and has not changed significantly over time in actual terms. There is need to develop and intensify community-based intervention strategies, programs for private hospitals, monitor MMR trends, identify and contextually address barriers at all levels of maternal care

Emergence and spread of two SARS-CoV-2 variants of interest in Nigeria.

Identifying the dissemination patterns and impacts of a virus of economic or health importance during a pandemic is crucial, as it informs the public on policies for containment in order to reduce the spread of the virus. In this study, we integrated genomic and travel data to investigate the emergence and spread of the SARS-CoV-2 B.1.1.318 and B.1.525 (Eta) variants of interest in Nigeria and the wider Africa region. By integrating travel data and phylogeographic reconstructions, we find that these two variants that arose during the second wave in Nigeria emerged from within Africa, with the B.1.525 from Nigeria, and then spread to other parts of the world. Data from this study show how regional connectivity of Nigeria drove the spread of these variants of interest to surrounding countries and those connected by air-traffic. Our findings demonstrate the power of genomic analysis when combined with mobility and epidemiological data to identify the drivers of transmission, as bidirectional transmission within and between African nations are grossly underestimated as seen in our import risk index estimates

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Size tunable synthesis of monodispersed hexadecylamine-capped CdSe nanostructures

We herein report the synthesis of high quality hexadecylamine (HDA)-capped CdSe nanostructures via a facile and effective non-organometallic method. By varying the growth temperature we monitored the size, optical properties and morphology of the as-synthesised CdSe nanoparticles. The nanoparticles were characterised using UV–vis absorption and photoluminescence (PL) spectroscopy, Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), transmission electron spectroscopy (TEM), high resolution electron transmission microscopy (HRTEM) and energy dispersive spectroscopy (EDS). The nanoparticles obtained were of high quality and monodispersed with high absorption and emission features. The emission and absorption maxima of the as-synthesised materials were red-shifted as the growth temperature increased indicating increase in particle size. The XRD analysis showed phase transition from cubic to hexagonal while the TEM images consist of both spherical and elongated particles as the temperature increased from 110 1C to 200 1C. The FTIR spectra confirmed the capping of the as-synthesised material by HDA while the EDS confirmed the presence of the corresponding elements.This work was supported by National Research Foundation (NRF), South Africa

FREE RANGE AND DEEP LITTER HOUSING SYSTEMS: EFFECT ON PERFORMANCE AND BLOOD PROFILE OF TWO STRAINS OF COCKEREL CHICKENS

This study was conducted to determine the performance and blood profile of one hundred and fifty cockerel chickens each of Harco Black and Novogen strains raised on deep litter and free range production systems. Each production system was allotted 150 chicks in three replications of 25 chicks per strain. The birds on deep litter production system were fed ad libitum while each of the birds on free range was fed 50 % of its daily feed requirement. The birds were weighed weekly. Blood plasma and serum were collected at the 4th and 12th weeks for laboratory analyses. Data generated were subjected to analysis of variance in a 2×2 factorial arrangement. Novogen strain consumed less feed (