FA profiles of maternal diet (A) and blood (B) according to maternal diet.

<p>HH is indicated by hatched columns and C diet by white columns. (A) FA concentrations of maternal diet were expressed in mg/100 g of food. In (B), 6 samples per group were used to evaluate SFA, MUFA and PUFA concentrations. (**p<0.01).</p

Relative expression of <i>LXR-α</i> expressed in arbitrary unit (A.U.) in HH and C placentas, according to sex.

<p>N = 5 per group. HH is indicated in hatched columns and C diet by white columns.</p

Relative gene expression in placentas according to the maternal diet.

<p>Ten placentas per group were used. Values are expressed as means ± standard error (SEM).</p

Morphological analysis of placentas on D28 of gestation and distribution of lipid droplets on D6 in blastocysts.

<p>(A-B) Light microscopy morphological analysis of placentas on D28 from C and HH fetuses. Numerous light vesicles (LV) are located in the trophoblastic layer (TL) of HH (B) compared to C placenta (A). A’ and B’ represent the high magnification of the zone indicated by a star. Scale bar: 100 µm. (C-D) Transmission electron microscopy of placentas on D28 of gestation from C and HH fetuses. Light vesicles were identified as lipid droplets (LD) in the trophoblastic layer (TL) of HH (right panel) compared to C placenta (left panel). EC: Endothelial cell; FV: Fetal Vessel; MC: Maternal Compartment. Scale bar: 5 µm. (E-F) Distribution of lipid droplets on D6 in C and HH blastocysts Fluorescent immunodetection of adipophilin (green), Nile red (red), and DNA (blue) from C (E) and HH (F) blastocysts. In HH blastocysts, abnormal accumulation of lipid droplets is observed around the nuclei (F’), colocalized with adipophilin staining (F’’), in contrast to C blastocysts (E-E”). Scale bar: 20 µm.</p

Relative gene expression in livers according to the maternal diet.

<p>Ten livers per group were used. Values are expressed as means ± standard error (SEM).</p

Placental quantification of SFA, MUFA and PUFA from membrane phospholipids and intracellular lipid storage expressed in mg/g of placenta in HH and C according to fetal sex.

<p>Six placentas per group were analyzed. Values are expressed as means ± standard error (SEM). (*p<0.05 and **p<0.01).</p

Effects of maternal diet on liver FA (A), SFA (B), MUFA (C) and PUFA (D) storage, according to sex.

<p>6 samples per group were analyzed. HH is indicated by hatched columns and C diet by white columns. (*p<0.05, **p<0.01 and ***p<0.001).</p

Gene-specific primers and their accession number.

<p>Gene-specific primers and their accession number.</p

Cholesteryl esters (A), triacylglycerol (B), membrane (C) and storage lipids (D) concentrations in C and HH placentas on D28 of gestation, according to fetal sex.

<p>For cholesteryl esters and triacylglycerol (TG) concentrations, 5 samples were used per group, whereas for FA concentrations, 6 samples per group were analyzed. HH is indicated by hatched columns and C diet by white columns. (*p<0.05 and **p<0.01).</p

Effects of maternal diet on total cholesterol (A), FA (B), SFA (C) and MUFA (D) concentrations in fetal plasma on D28 of gestation, according to sex.

<p>For total cholesterol concentrations, 12 males and 11 female fetal samples from HH does (hatched columns) and 14 males and 12 females from C does (white columns) were used. For FA, SFA and MUFA concentrations, 6 samples by group were analyzed. (*p<0.05 and **p<0.01)</p