Human FoxP3^<b>+</b> CD4 T cells express high levels of IL-2 Rβ.

<p>IL-2Rβ (<b>Fig. 2A, 2B</b>), IL-2Rγ (<b>Fig. 2A, 2C</b>), IL-15Rα (<b>Fig. 2A, 2D</b>) and IL-7Rα (<b>Fig. 2A, 2E</b>) expression was analyzed by flow cytometry in FoxP3⁺ and FoxP3⁻ CD4 T cells before liver grafting and basiliximab injection (Figs. 2B to 2E), and at various times thereafter (2B to 2E). Fig. A shows a representative pre-graft staining profile. Figs. 2B to 2E represent the mean (SEM) MFI ratios (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011784#s3" target="_blank">Methods</a>). The Wilcoxon test was used to compare FoxP3⁺ and FoxP3⁻ CD4 T cells. The Mann Whitney test was used to analyze the time course of expression in a given cell subset. Only significant differences are indicated.</p

No significant effect of a blocking anti-CD25 antibody on the proportion of human FoxP3^<b>+</b> CD4 T cells in vivo.

<p>The proportion of CD25⁺FoxP3⁺ (<b>1A</b>), CD25⁻FoxP3⁺ (<b>1B</b>) and total FoxP3⁺ cells (<b>1C</b>) among CD4 T lymphocytes was determined before liver grafting and basiliximab injection, and for more than 9 months thereafter; data are means (curves) and SEM at the different time points. In <b>1D</b>, the FoxP3⁺/FoxP3⁻ and FoxP3^hi/FoxP3⁻ MFI ratios were used to compare the level of intracellular FoxP3 expression (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011784#s3" target="_blank">Methods</a>). Means and SEM are shown. The Mann Whitney test was used for statistical analysis. The number of patients tested at each time point is indicated. Only statistically significant differences are indicated.</p