Ciliary body melanoma.

<p>A – Photograph: iris with visible melanoma at 1 o'clock (arrow). B – UBM of patient after ruthenium-106 plaque brachytherapy: residual tumor mass with height of 2.7 mm (arrow). C+D – Sagittal and axial T2w images: Inhomogeneous tumor invading the ciliary body (arrow). Low-signal-intensity cap-shaped mass representing the melanin-producing portion of the tumor. No scleral invasion.</p

83-year-old patient with choroidal melanoma infiltrating the retrobulbar fat.

<p>A – Sonography: dome-shaped tumor of low reflectivity with a highly reflective surface. No information on extraocular tumor extension. B – H&E stain, 2× magnification: infiltration of the retrobulbar fat as demonstrated by MRM (arrow). C – Sagittal T2w image also demonstrates infiltration of the retrobulbar fat (arrow).</p

Subretinal bleeding with degenerative, atrophic choroid and retina.

<p>A – Sonography: highly reflective and detached cystic retina and choroidea (arrow); subretinal space with low internal reflectivity. B – H&E stain, 2× magnification: Peripapillary subretinal mass (arrow) with degenerative and atrophic lesions of the retina (hash) and choroid (asterisk). C – Sagittal T2w image: Subretinal mass of very low signal intensity with retinal detachment, fluid-fluid level (arrow), pseudophakia (arrowhead).</p

Confocal laser scanning microscopy of the urinary bladder.

<p><i>In vivo</i> confocal laser scanning microscopy of the bladder showing eggs of <i>Schistosoma haematobium</i> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034869#pone-0034869-g001" target="_blank">figure 1a and 1b</a>) with their typical terminal spine (arrow) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034869#pone-0034869-g001" target="_blank">figure 1b</a>).</p

Hemorrhagic choroidal detachment (arrow).

<p>A – Sonography: Kissing bullae with high internal reflectivity. B – H&E stain, 2x: Portions of detached retina and choroid with blood underneath. C – Coronal T2w image: Very low internal signal intensity due to the presence of hemoglobin degradation products, fluid-fluid level in the lateral compartment.</p

Immunocytochemical characterization of TGF-β1 induced fibronectin and α-SMA expression in primary hOFs <i>in vitro</i>.

<p>After PFA fixation, cells were incubated with primary antibodies directed against fibronectin and α-SMA. Nuclei were stained by DAPI included in the mounting medium. PFD decreased fibronectin and α-SMA expression. Experiments were carried out four times with similar results. Bar represents 50 μm.</p

Immunocytochemical characterization of TGF-β1 induced fibronectin and α-SMA expression in primary hTFs <i>in vitro</i>.

<p>After PFA fixation, cells were incubated with primary antibodies directed against fibronectin and α-SMA. Nuclei were stained by DAPI included in the mounting medium. PFD decreased fibronectin and α-SMA expression. Experiments were carried out four times with similar results. Bar represents 50 μm.</p

Relative proliferation of hTFs and hOFs in response to TGF-β1 and PFD <i>in vitro</i>.

<p>Fibroblasts were treated with TGF-β1 [10 ng/ml], PFD [10⁻³ mol/l] or the combination of TGF-β1 [10 ng/ml] and PFD [10⁻³ mol/l] for 48 h under serum-free (A and C) and serum (10% FCS) conditions (B and D) as indicated. NC (proliferation rate of untreated cells) was set to 100%. Data are presented as mean ± SD. The results represent the means of three independent experiments. Level of significances: *p≤0.05; **p≤0.01; ***p≤0.001; ****p≤0.0001.</p

Human fibroblast subpopulations <i>in vitro</i>.

<p>Primary fibroblast subpopulations from Tenon’s capsule (hTFs) and fibroblasts from orbital fat (hOFs) were cultured for 1, 3 and 7 days, respectively. div = days <i>in vitro</i>. Bar represents 25 μm.</p

RT-PCR analysis of stimulated (TGF-β1) and suppressed (PFD) primary hOFs.

<p>Cultures of hOFs were treated with TGF-β1 [10 ng/ml], PFD [10⁻³ mol/l] or the combination of both, TGF-β1 and PFD for 48 h under serum-free culture conditions. NC (expression level of respective gene in untreated cells, dotted line) was set to 1. Data are presented as mean ± SD. The results represent the means of four independent experiments. Level of significances: *p≤0.05; **p≤0.01; ***p≤0.001. Overall, no statistically significant differences between TGF-β1 and TGF-β1+PFD groups could be observed.</p