Preferential Signaling and Induction of Allergy-promoting Lymphokines Upon Weak Stimulation of the High Affinity IgE Receptor on Mast Cells

Mast cell degranulation and de novo cytokine production is a consequence of antigen-aggregation of the immunoglobulin E (IgE)-occupied high affinity receptor for IgE (FcɛRI). Herein, we report that lymphokines that promote allergic inflammation, like MCP-1, were potently induced at low antigen (Ag) concentrations or at low receptor occupancy with IgE whereas some that down-regulate this response, like interleukin (IL)-10, required high receptor occupancy. Weak stimulation of mast cells caused minimal degranulation whereas a half-maximal secretory response was observed for chemokines and, with the exception of TNF-α, a weaker cytokine secretory response was observed. The medium from weakly stimulated mast cells elicited a monocyte/macrophage chemotactic response similar to that observed at high receptor occupancy. Weak stimulation also favored the phosphorylation of Gab2 and p38MAPK, while LAT and ERK2 phosphorylation was induced by a stronger stimulus. Gab2-deficient mast cells were severely impaired in chemokine mRNA induction whereas LAT-deficient mast cells showed a more pronounced defect in cytokines. These findings demonstrate that perturbation of small numbers of IgE receptors on mast cells favors certain signals that contribute to a lymphokine response that can mediate allergic inflammation

Composição morfológica e resposta produtiva de acessos do gênero Manihot.

Neste estudo, objetivou-se avaliar a composição morfológica e a resposta produtiva de acessos de Manihot sp

RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote.

Heterotrimeric G protein alpha subunits, RGS proteins, and GoLoco motif proteins have been recently implicated in the control of mitotic spindle dynamics in C. elegans and D. melanogaster. Here we show that regulator of G protein signaling-14 (RGS14) is expressed by the mouse embryonic genome immediately prior to the first mitosis, where it colocalizes with the anastral mitotic apparatus of the mouse zygote. Loss of Rgs14 expression in the mouse zygote results in cytofragmentation and failure to progress to the 2-cell stage. RGS14 is found in all tissues and segregates to the nucleus in interphase and to the mitotic spindle and centrioles during mitosis. Alteration of RGS14 levels in exponentially proliferating cells leads to cell growth arrest. Our results indicate that RGS14 is one of the earliest essential product of the mammalian embryonic genome yet described and has a general role in mitosis

Genetic diversity of Macroptilium accessions considering the increase in air temperature.

Climate changes can influence the genetic diversity of forage plants, which may contribute to theimprovement and development of new species. Therefore, this research aimed to evaluate the influence of temperature increase on the genetic diversity of Macroptilium accessions based on morphoagronomic descriptors. The experiment was carried out in a growth chamber in a 2×16 factorial arrangement (temperature regimes x Macroptilium accessions), with the temperatures consisting of T1 (20?26?33 °C) and T2 (24.8?30.8?37.8 °C) and 16 accessions. Eleven morphoagronomic descriptors allowed estimating the diversity among accessions. The measurements of genetic dissimilarity enabled us to observe the genetic distance between the studied materials, standing out the accessions T1.M3 and T2.S4 as the most divergent (446.01). The morphoagronomic descriptors percentage of leaves and stem diameter were the most efficient for estimating the diversity between access. Genetic variability points to the adaptation of Macroptilium accessions in the climate change scenario. The accessions more divergent can be explored in genetic breeding programs for the species aiming at the expansion of genetic variability as an adaptation mechanism to heat stress

Resistência a Didymella bryoniae em genótipos de melão.

O objetivo do 14 presente trabalho foi avaliar e obter descendentes com resistência a D. bryoniae em 15 genótipos de melão do programa de melhoramento da Embrapa

Triagem Inteligente: O Uso da Internet das Coisas na Classificação dos Riscos nas Emergências

Este artigo tem como objetivo apresentar a automação do processo de triagem de pacientes utilizando o atuador Arduino. O processo de triagem determina o nível de risco do paciente e sua execução pode tornar-se desnecessariamente complexa, sendo que, aproximadamente, 80% dos pacientes em serviços de emergência não necessitam de atendimento urgente. Portanto, a automatização deste processo pode provar-se bastante benéfica: os dados do paciente são analisados em tempo real, provendo um resultado seguro e em alta velocidade, garantindo prioridade a pacientes em risco e reduzindo o tempo de admissão dele

Triagem Inteligente: O Uso da Internet das Coisas na Classificação dos Riscos nas Emergências

Este artigo tem como objetivo apresentar a automação do processo de triagem de pacientes utilizando o atuador Arduino. O processo de triagem determina o nível de risco do paciente e sua execução pode tornar-se desnecessariamente complexa, sendo que, aproximadamente, 80% dos pacientes em serviços de emergência não necessitam de atendimento urgente. Portanto, a automatização deste processo pode provar-se bastante benéfica: os dados do paciente são analisados em tempo real, provendo um resultado seguro e em alta velocidade, garantindo prioridade a pacientes em risco e reduzindo o tempo de admissão dele

Fiber Optical Cable and Connector System (FOCCoS) for PFS/Subaru

FOCCoS, Fiber Optical Cable and Connector System, has the main function of capturing the direct light from the focal plane of Subaru Telescope using optical fibers, each one with a microlens in its tip, and conducting this light through a route containing connectors to a set of four spectrographs. The optical fiber cable is divided in 3 different segments called Cable A, Cable B and Cable C. Multi-fibers connectors assure precise connection among all optical fibers of the segments, providing flexibility for instrument changes. To assure strong and accurate connection, these sets are arranged inside two types of assemblies: the Tower Connector, for connection between Cable C and Cable B; and the Gang Connector, for connection between Cable B and Cable A. Throughput tests were made to evaluate the efficiency of the connections. A lifetime test connection is in progress. Cable C is installed inside the PFI, Prime Focus Instrument, where each fiber tip with a microlens is bonded to the end of the shaft of a 2-stage piezo-electric rotatory motor positioner; this assembly allows each fiber to be placed anywhere within its patrol region, which is 9.5mm diameter.. Each positioner uses a fiber arm to support the ferrule, the microlens, and the optical fiber. 2400 of these assemblies are arranged on a motor bench plate in a hexagonal-closed-packed disposition.Comment: 11 pages, 20 figure