CIÊNCIA E SENSO COMUM: BOAVENTURA E AS CRÍTICAS A VISÃO BACHELARDIANA

Este trabalho se propõe a analisar as críticas de Santos acerca da relação ciência e senso comum de Gaston Bachelard. Nesse sentido, Santos argumenta que a construção epistemológica bachelardiana defende o rompimento da cultura científica com o senso comum. Dito isso, o senso comum acaba sendo diminuído em detrimento do conhecimento científico e este trazendo uma imagem inadequada tanto do senso comum, quanto da própria ciência. Logo, Santos acredita que estamos caminhando para uma nova relação entre ciência e senso comum, em que uma cultura faz parte da outra e ambas constroem uma nova proposta de conhecimento

Multifocal tuberculosis-associated immune reconstitution inflammatory syndrome – a case report of a complicated scenario

Background Tuberculosis (TB)-associated Immune reconstitution inflammatory syndrome (TB-IRIS) is an aberrant inflammatory response in TB patients with advanced human immunodeficiency virus coinfection, after antiretroviral therapy commencement. Case presentation We present a rare case of a 51-year-old woman living with HIV who developed a series of TB-IRIS events occurring at multiple sites sequentially, highlighting the clinical complexity in diagnosis and management. Conclusion This case illustrates how complicated a clinical scenario of successive TB-IRIS episodes can be, in terms of clinical management

Association between Immunophenotypic Parameters and Molecular Alterations in Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a hematologic malignancy that occurs due to alterations such as genetic mutations, chromosomal translocations, or changes in molecular levels. These alterations can accumulate in stem cells and hematopoietic progenitors, leading to the development of AML, which has a prevalence of 80% of acute leukemias in the adult population. Recurrent cytogenetic abnormalities, in addition to mediating leukemogenesis onset, participate in its evolution and can be used as established diagnostic and prognostic markers. Most of these mutations confer resistance to the traditionally used treatments and, therefore, the aberrant protein products are also considered therapeutic targets. The surface antigens of a cell are characterized through immunophenotyping, which has the ability to identify and differentiate the degrees of maturation and the lineage of the target cell, whether benign or malignant. With this, we seek to establish a relationship according to the molecular aberrations and immunophenotypic alterations that cells with AML present

Differential expression of CXCR3 and CCR6 on CD4+ T-lymphocytes with distinct memory phenotypes characterizes tuberculosis-associated immune reconstitution inflammatory syndrome

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2019-03-20T12:22:30Z No. of bitstreams: 1 Mattos P.S.S Differential expression ...2019.pdf: 2847136 bytes, checksum: c7e111e870e41637cee13cd43c536281 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-03-20T13:02:59Z (GMT) No. of bitstreams: 1 Mattos P.S.S Differential expression ...2019.pdf: 2847136 bytes, checksum: c7e111e870e41637cee13cd43c536281 (MD5)Made available in DSpace on 2019-03-20T13:02:59Z (GMT). No. of bitstreams: 1 Mattos P.S.S Differential expression ...2019.pdf: 2847136 bytes, checksum: c7e111e870e41637cee13cd43c536281 (MD5) Previous issue date: 2019-01-06Intramural Research Program of NIAID/NIH and by the Intramuralto- India grant from the US-India Co-operative research program. This study was also financed in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Finance Code 001). The work of B.B.A. was supported by a grant from NIH (U01AI115940). P.S.S.M. was supported by a PhD fellowship from the Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). K.F.F. received a fellowship from the Programa Nacional de Pós-Doutorado, CAPES. C.V. and D.S. are supported by FAPESBFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil.National Institute for Research in Tuberculosis. Chennai, India.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / University of California. Department of Medicine. Division of Infectious Diseases. San Diego, United States of America.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil.National Institute for Research in Tuberculosis. Chennai, India.National Institute for Research in Tuberculosis. Chennai, India.National Institute for Research in Tuberculosis. Chennai, India.National Institute for Research in Tuberculosis. Chennai, India.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Government Hospital of Thoracic Medicine. Tambaram, Chennai, India.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Immunoregulation. Clinical HIV Pathogenesis Section. Bethesda, Maryland, United States of America.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Immunobiology Section. Bethesda, Maryland, United States of America.National Institute for Research in Tuberculosis. Chennai, India.Government Hospital of Thoracic Medicine. Tambaram, Chennai, India.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil / University of Cape Town. Institute of Infectious Disease and Molecular Medicine. Wellcome Trust Centre for Infectious Disease Research in Africa. Cape Town, Republic of South Africa / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Universidade Salvador. Laureate Universities. Salvador, BA, Brasil / Vanderbilt University School of Medicine. Division of Department of Medicine. Infectious Diseases. Nashville, Tennessee, United States of America.Immune reconstitution inflammatory syndrome (IRIS) occurs in up to 40% of individuals co-infected with pulmonary tuberculosis (PTB) and HIV, primarily upon antiretroviral therapy (ART) initiation. Phenotypic changes in T-cells during TB-IRIS and their relationship with systemic inflammation are not fully understood. In this prospective cohort study, we followed 48 HIV-positive patients with PTB from South India before and after ART initiation, examining T-lymphocyte subsets and inflammatory biomarkers in peripheral blood. Quantification of naïve (CD27+CD45RO-) as well as effector memory CD4+ T cells (CD27-CD45RO+) at weeks 2-6 after ART initiation could distinguish TB-IRIS from non-IRIS individuals. Additional analyses revealed that ART reconstituted different quantities of CD4+ T lymphocyte subsets with preferential expansion of CXCR3+ CCR6- cells in TB-IRIS patients. Moreover, there was an expansion and functional restoration of central memory (CD27+CD45RO+) CXCR3+CCR6- CD4+ lymphocytes and corresponding cytokines, with reduction in CXCR3-CCR6+ cells after ART initiation only in those who developed TB-IRIS. Together, these observations trace a detailed picture of CD4+ T cell subsets tightly associated with IRIS, which may serve as targets for prophylactic and/or therapeutic interventions in the future

Molecular degree of perturbation of plasma inflammatory markers associated with tuberculosis reveals distinct disease profiles between Indian and Chinese populations

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2019-08-01T12:15:41Z No. of bitstreams: 1 Souza, D.O. Molecular degree of perturbation...2019.pdf: 1729409 bytes, checksum: ab65dea396416432df6627a7b3160e01 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-08-01T12:42:19Z (GMT) No. of bitstreams: 1 Souza, D.O. Molecular degree of perturbation...2019.pdf: 1729409 bytes, checksum: ab65dea396416432df6627a7b3160e01 (MD5)Made available in DSpace on 2019-08-01T12:42:19Z (GMT). No. of bitstreams: 1 Souza, D.O. Molecular degree of perturbation...2019.pdf: 1729409 bytes, checksum: ab65dea396416432df6627a7b3160e01 (MD5) Previous issue date: 2019-01-29Fundação Oswaldo Cruz, Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz, Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz, Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil.National Institutes of Health- National Institute for Research in Tuberculosis. International Center for Excellence in Research. India.Fundação Oswaldo Cruz, Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil.Henan Chest Hospital. Zhengzhou, China.Henan Chest Hospital. Zhengzhou, China.Henan Chest Hospital. Zhengzhou, China.Sino-US International Research Center for Tuberculosis, and Henan Public Health Center. Zhengzhou, China.Laboratory of Clinical Immunology and Microbiology. Bethesda, USA.Laboratory of Clinical Immunology and Microbiology. Bethesda, USA.Laboratory of Clinical Immunology and Microbiology. Bethesda, USA.Laboratory of Parasitic Diseases. Bethesda, USA.National Institutes of Health- National Institute for Research in Tuberculosis. International Center for Excellence in Research. India.Laboratory of Clinical Immunology and Microbiology. Bethesda, USA.University of São Paulo. School of Pharmaceutical Sciences. Department of Pathophysiology and Toxicology. São Paulo, SP, Brazil.Fundação Oswaldo Cruz, Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz, Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil / Vanderbilt University School of Medicine. Division of Infectious Diseases. Department of Medicine.Tuberculosis (TB) is a chronic inflammatory disease caused by Mycobacterium tuberculosis infection which causes tremendous morbidity and mortality worldwide. Clinical presentation of TB patients is very diverse and disease heterogeneity is associated with changes in biomarker signatures. Here, we compared at the molecular level the extent of individual inflammatory perturbation of plasma protein and lipid mediators associated with TB in patients in China versus India. We performed a cross-sectional study analyzing the overall degree of inflammatory perturbation in treatment-naïve pulmonary TB patients and uninfected individuals from India (TB: n = 97, healthy: n = 20) and China (TB: n = 100, healthy: n = 11). We employed the molecular degree of perturbation (MDP) adapted to plasma biomarkers to examine the overall changes in inflammation between these countries. M. tuberculosis infection caused a significant degree of molecular perturbation in patients from both countries, with higher perturbation detected in India. Interestingly, there were differences in biomarker perturbation patterns and the overall degree of inflammation. Patients with severe TB exhibited increased MDP values and Indian patients with this condition exhibited even higher degree of perturbation compared to Chinese patients. Network analyses identified IFN-α, IFN-β, IL-1RI and TNF-α as combined biomarkers that account for the overall molecular perturbation in the entire study population. Our results delineate the magnitude of the systemic inflammatory perturbation in pulmonary TB and reveal qualitative changes in inflammatory profiles between two countries with high disease prevalence