A new approach to assess function after sciatic nerve lesion in the mouse—Adaptation of the sciatic static index

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2016-07-04T17:30:40Z No. of bitstreams: 1 Batista AF A new aproach to assess......pdf: 398143 bytes, checksum: 2681ad3389c39178e4f588bd7ae03e9b (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2016-07-04T17:54:15Z (GMT) No. of bitstreams: 1 Batista AF A new aproach to assess......pdf: 398143 bytes, checksum: 2681ad3389c39178e4f588bd7ae03e9b (MD5)Made available in DSpace on 2016-07-04T17:54:15Z (GMT). No. of bitstreams: 1 Batista AF A new aproach to assess......pdf: 398143 bytes, checksum: 2681ad3389c39178e4f588bd7ae03e9b (MD5) Previous issue date: 2007Made available in DSpace on 2016-07-14T19:07:29Z (GMT). No. of bitstreams: 3 Batista AF A new aproach to assess......pdf.txt: 30533 bytes, checksum: f642238d7fa4d2895ade3d42be875500 (MD5) Batista AF A new aproach to assess......pdf: 398143 bytes, checksum: 2681ad3389c39178e4f588bd7ae03e9b (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) Previous issue date: 2007Universidade Federal do Rio de Janeiro. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil / Fundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, BrasilAmong the numerous ways of assessing regeneration after peripheral nerve lesions, the analysis of gait is one of the most important, because it shows the recovery of function, which is the ultimate goal of the repair machinery. The sciatic function index was introduced as a method to assess reinnervation after an experimental sciatic nerve lesion, and was adapted to the mouse model. The sciatic static index (SSI), is more simple and practical to perform, and is not so influenced by gait’s velocity, but this method has not yet been adapted to the mouse model of sciatic lesion. We used 63 male Swiss mice (Mus musculus) to develop a formula to the sciatic static index in mice (SSIm). The animals were divided on three groups (control, transection and crush). They were evaluated at the preoperative and 7th, 14th, 21st, 28th, 35th and 42nd days postoperative by the ink track method (SFI), and by the acquisition of photographs of the plantar aspects of the injured and uninjured hind paws. The parameters evaluated were the 1–5 toe spread (TS), the 2–4 toe spread (ITS) and the distance between the tip of the third toe and the most posterior aspect of the paw (PL), on both methods. After verifying the temporal pattern of function, correlation and reproducibility of the measurements, we performed a multiple regression analysis using SFI values as dependent variable, and the TS, ITS and PL measured with the photo method as independent variables, and found the formula of the SSI for mice (SSIm). The three groups (control, transection and crush) had a characteristic pattern of dysfunction. The parameters measured in the ink and photo method had variable but significant correlations between them (P < 0.000), but photo method of measurement showed a better reproducibility. The correlation between SFI and SSIm showed a high correlation coefficient (r = 0.892, P < 0.000), and demonstrates that SSIm can be used as an alternative method to assess the functional status relative of sciatic nerve activity in mice