Septic Tank Survey: 2018

Between May 2018 and September 2018, the Institute for Social and Behavioral Science (ISBS) at the University of Central Florida (UCF) surveyed residents of Brevard County, Florida to explore their behaviors and attitudes on a variety of septic tank related topics

Teachers\u27 Perceptions of Safety Regarding School Shootings

As a result of high media attention surrounding school shootings in recent years, it may appear that American public schools are becoming dangerous places (Schildkraut & Elsass, 2016; Elsass, Schildkraut, & Stafford, 2016; Toppo, 2013). Though schools remain to be one of the safest locations for children, various safety measures are discussed and implemented in schools to combat this perceived problem and ensure the safety of school campuses. Discussions of best safety practices spikes directly following a school shooting event from relevant parties, such as school administration, law enforcement agencies, parents, and students, (Crawford & Burns, 2015; Chrusciel, Wolfe, Hansen, Rojek, & Kaminski, 2014) but little existing literature focuses on teachers, more specifically how safe teachers perceive schools to be with regard to gun violence and school shootings. This study seeks to build upon the small body of literature that currently exists on teachers\u27 perceptions of safety and hopes to introduce new opportunities for research in the future. This study is comprised of 212 teachers throughout Central Florida. The results of this research show that while teachers overall feel very safe teaching at their schools, there are areas they believe can be improved upon. Of the safety measures used to prevent and reduce gun violence at schools, program-based safety measures and School Resource Officers make these teachers feel the safest. These perceptions of safety do not vary from one Central Florida county to the next, as most teachers are largely in agreement as to what measures make them feel safe. It was also revealed that most teachers in the sample are also unsupportive of legislation that would allow classroom teachers to carry firearms on school campuses

The Parks in the City of Winter Park 2018: Executive Summary

Between June 2018 and July 2018, 325 Winter Park residents were surveyed about their opinions of the City of Winter Park’s parks, how they used the parks, their specific neighborhood parks, and any changes they would like to see regarding the parks. Surveys were conducted over the telephone by trained ISBS employees. ISBS received a list of Winter Park residents’ phone numbers from the utility company to allow for the survey to be conducted

Senior Resource Alliance Needs Assessment 2018: Final Report

The University of Central Florida’s Institute for Social and Behavioral Science (ISBS) conducted a needs assessment on behalf of the Senior Resource Alliance. The needs assessment consisted of a survey with 400 senior respondents in PSA 7 as well as secondary data analysis. Specifically the secondary data analysis focused on three components: 1. Demographic report on the population age 60+, 65+, and 85+, showing the most recent data at the local, state, and national level; 2. Statistical summaries of target populations and target areas in PSA 7; and 3. Population projections on the baby boomer cohort data. The University of Central Florida’s Institute for Social and Behavioral Sciences (ISBS) conducted a needs assessment on behalf of the Senior Resource Alliance. The needs assessment consisted of a survey with 400 senior respondents in PSA 7 as well as secondary data analysis. Specifically the secondary data analysis focused on three components: 1. Demographic report on the population age 60+, 65+, and 85+, showing the most recent data at the local, state, and national level; 2. Statistical summaries of target populations and target areas in PSA 7; and 3. Population projections on the baby boomer cohort data

Host-pathogen genetic interactions underlie tuberculosis susceptibility in genetically diverse mice [preprint]

The outcome of an encounter with Mycobacterium tuberculosis (Mtb) depends on the pathogen’s ability to adapt to the heterogeneous immune response of the host. Understanding this interplay has proven difficult, largely because experimentally tractable small animal models do not recapitulate the heterogenous disease observed in natural infections. We leveraged the genetically diverse Collaborative Cross (CC) mouse panel in conjunction with a library of Mtb mutants to associate bacterial genetic requirements with host genetics and immunity. We report that CC strains vary dramatically in their susceptibility to infection and represent reproducible models of qualitatively distinct immune states. Global analysis of Mtb mutant fitness across the CC panel revealed that a large fraction of the pathogen’s genome is necessary for adaptation to specific host microenvironments. Both immunological and bacterial traits were associated with genetic variants distributed across the mouse genome, elucidating the complex genetic landscape that underlies host-pathogen interactions in a diverse population

Tuberculosis susceptibility and vaccine protection are independently controlled by host genotype

The outcome of Mycobacterium tuberculosis infection and the immunological response to the bacillus Calmette-Guerin (BCG) vaccine are highly variable in humans. Deciphering the relative importance of host genetics, environment, and vaccine preparation for the efficacy of BCG has proven difficult in natural populations. We developed a model system that captures the breadth of immunological responses observed in outbred individual mice, which can be used to understand the contribution of host genetics to vaccine efficacy. This system employs a panel of highly diverse inbred mouse strains, consisting of the founders and recombinant progeny of the "Collaborative Cross" project. Unlike natural populations, the structure of this panel allows the serial evaluation of genetically identical individuals and the quantification of genotype-specific effects of interventions such as vaccination. When analyzed in the aggregate, our panel resembled natural populations in several important respects: the animals displayed a broad range of susceptibility to M. tuberculosis, differed in their immunological responses to infection, and were not durably protected by BCG vaccination. However, when analyzed at the genotype level, we found that these phenotypic differences were heritable. M. tuberculosis susceptibility varied between lines, from extreme sensitivity to progressive M. tuberculosis clearance. Similarly, only a minority of the genotypes was protected by vaccination. The efficacy of BCG was genetically separable from susceptibility to M. tuberculosis, and the lack of efficacy in the aggregate analysis was driven by nonresponsive lines that mounted a qualitatively distinct response to infection. These observations support an important role for host genetic diversity in determining BCG efficacy and provide a new resource to rationally develop more broadly efficacious vaccines. IMPORTANCE Tuberculosis (TB) remains an urgent global health crisis, and the efficacy of the currently used TB vaccine, M. bovis BCG, is highly variable. The design of more broadly efficacious vaccines depends on understanding the factors that limit the protection imparted by BCG. While these complex factors are difficult to disentangle in natural populations, we used a model population of mice to understand the role of host genetic composition in BCG efficacy. We found that the ability of BCG to protect mice with different genotypes was remarkably variable. The efficacy of BCG did not depend on the intrinsic susceptibility of the animal but, instead, correlated with qualitative differences in the immune responses to the pathogen. These studies suggest that host genetic polymorphism is a critical determinant of vaccine efficacy and provide a model system to develop interventions that will be useful in genetically diverse populations.This work, including the efforts of Hardy Kornfeld, was funded by HHS | National Institutes of Health (NIH) (HL081149). This work, including the efforts of Sam Behar, was funded by HHS | National Institutes of Health (NIH) (AI123286-01). This work, including the efforts of Clare Margaret Smith and Christopher Sassetti, was funded by Howard Hughes Medical Institute (HHMI)

Exploring the potential for dual vaccination against Hepatitis B virus and Helicobacter pylori using a recombinant virus like particle

HBsAg VLPs are highly immunogenic due to their particulate nature and repetitive sub-unit structure. We describe a novel delivery system which is also the first report of recombinant VLPs able to stimulate protective immune responses to a bacterial pathogen

Effects of abstinence-only sex education on UTC college students

The purpose of this research is to assess the effectiveness of abstinence-only education in providing participants with medically accurate knowledge and to assess participants’ perspectives on the impact of the sex education they received

Increases in Doublecortin Immunoreactivity in the Dentate Gyrus following Extinction of Heroin-Seeking Behavior

Adult-generated neurons in the dentate gyrus (DG) of the hippocampus play a role in various forms of learning and memory. However, adult born neurons in the DG, while still at an immature stage, exhibit unique electrophysiological properties and are also functionally implicated in learning and memory processes. We investigated the effects of extinction of drug-seeking behavior on the formation of immature neurons in the DG as assessed by quantification of doublecortin (DCX) immunoreactivity. Rats were allowed to self-administer heroin (0.03 mg/kg/infusion) for 12 days and then subjected either to 10 days of extinction training or forced abstinence. We also examined extinction responding patterns following heroin self-administration in glial fibrillary acidic protein thymidine kinase (GFAP-tk) transgenic mice, which have been previously demonstrated to show reduced formation of immature and mature neurons in the DG following treatment with ganciclovir (GCV). We found that extinction training increased DCX immunoreactivity in the dorsal DG as compared with animals undergoing forced abstinence, and that GCV-treated GFAP-tk mice displayed impaired extinction learning as compared to saline-treated mice. Our results suggest that extinction of drug-seeking behavior increases the formation of immature neurons in the DG and that these neurons may play a functional role in extinction learning

HIV gp120 impairs nucleus accumbens neuroimmune function and dopamine D3 receptor-mediated inhibition of cocaine seeking in male rats

Cocaine Use Disorders (CUDs) are associated with an increased risk of human immunodeficiency virus (HIV) infection. Cocaine and the HIV envelope protein gp120 each induce distinct deficits to mesocorticolimbic circuit function and motivated behavior; however, little is known regarding how they interact to dysregulate these functions or how such interactions impact pharmacotherapeutic efficacy. We have previously shown that the selective, weak partial agonist of the dopamine D3 receptor (D3R), MC-25-41, attenuates cocaine-seeking behavior in male rats. Here, we sought to characterize changes in striatal neuroimmune function in gp120-exposed rats across abstinence from operant access to cocaine (0.75 mg/kg, i.v.) or sucrose (45 mg/pellet), and to examine the impact of gp120 exposure on MC-25-41-reduced cocaine seeking. After establishing a history of cocaine or sucrose self-administration, rats received intracerebroventricular gp120 infusions daily the first 5 days of abstinence and were sacrificed either on day 6 or after 21 days of forced abstinence and a cue-induced cocaine seeking test. We demonstrated that MC-25-41 treatment attenuated cue-induced cocaine seeking among control rats but not gp120-exposed rats. Moreover, postmortem analysis of nucleus accumbens (NAc) core neuroimmune function indicated cocaine abstinence- and gp120-induced impairments, and the expression of several immune factors within the NAc core significantly correlated with cocaine-seeking behavior. We conclude that cocaine abstinence dysregulates striatal neuroimmune function and interacts with gp120 to inhibit the effectiveness of a D3R partial agonist in reducing cocaine seeking. These findings highlight the need to consider comorbidities, such as immune status, when evaluating the efficacy of novel pharmacotherapeutics